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M I C
1.
2. 1- Ali Moubarak Ali
2- Islam Hasan Abdelsalam
3- Ahmed Gamal Abdelmola
4- Mena Tarek
5- Yasser Mohi
Under Supervision of :
DR : Ahmed Osama ELgendy.
3. What is the definition of MIC ?
Importance of using MIC
Materials used for determination of MIC
Methods used for determination of MIC
Results obtained from Experiment
Iterpretation of results
Conclusion of the study
Items of our project
References
4.
5. Different species of microorganisms have varying
degrees of susceptibility to antimicrobial agents may
be { sensitive - intermediate - resistant } . Further, the
pathogenic microbes may develop drug resistance to a
particular type of antimicrobial agent on prolonged use.
the antimicrobial sensitivity tests are very useful to
determine the level of antimicrobial activity of a
particular chemical compound on certain pathogenic
microorganisms using agar diffusion and dilution
method.
Anti-microbial activity
6. Minimum Inhibitory Concentration (MIC)
It is the lowest concentration of the
antimicrobial agent that inhibits the growth of
the test organism but not necessarily kills it.
Methods for determination of MIC
1. Serial dilution method :
a- Broth dilution method. b- Agar dilution method.
1. -----------------------------------------
2. Agar diffusion method :
a-Cup plate technique. b- Gradient plate technique.
c- Ditch plate technique.
12. a) Subculture for 9 indicator microorganisms in
Brain Heart Infusion broth (B.H.I.) and incubated
for 24 hr. at 37 °C. These M.O were Salmonella ,
Bacillus , Pseudomonas , E.coli , Candida , M.Pheli ,
Sarcina , Staph. Aureus and Enterococcus.
b) All samples were dissolved in DMSO to give a
final stock concentration of 10 mg/ml.
13. c) MIC determination :
i. Preparation of 1 L Muller Hinton Agar media.
ii. Using a pre-marked 10 ml sterile test tubes, we were
able to transfer the media to petri dish plates.
iii. Diff conc. of each sample in ascending manner 12.5
, 25 , 50 , 100 , 200 µg/ml in 10 ml agar were prepared
and poured in petri dishes.
iv. Using a multi-inoculator we were able to add 9 M.O
at once then incubated for 24 hr. at 37 °C .
14.
15. Results were recorded in terms of MIC , which is the
lowest concentration of antimicrobial agents causing almost
complete inhibition of growth or giving no visible growth.
Growth of all 9 M.O at conc of 200 µg/ml so conc of 200 µg/ml
not able to inhibit growth of all M.O MIC of all > 200
9
1
16. 8
1 2
3
4
8
1 2
8
1
2
- In 8 * no growth from a conc of
200 µg/ml to a conc of 25 µg/ml
but there is a growth at a conc of
12.5 µg/ml so MIC = 25 .
- In 1 , 3 , 4 * there is a microbial growth
in a conc of 200 µg/ml so MIC > 200 .
- In 2 * no growth at 200 µg/ml
& there is a growth at 25 µg/ml
so MIC ≤ 200 & > 25 .
{ may be 200 or 100 or 50 }
18. • Each M.O was screened for its growth starting from
a conc of 200 µg/ml to a conc of 12.5 µg/ml plate
at which :
12.52550100200
+ ve+ ve- ve- ve- ve
A conc of (µg/ml)
Growth
Arising of first growth is an indicator of Sub_MIC .
Last plate showing no growth is an indicator of MIC .
Interpretation of results
Sub_MIC = 25 MIC = 50
19. The agar dilution method was most fitted over
Broth macro & micro dilution methods because :
This method has many advantages as :
Evaluate the agent against many microorganisms at
once .
Turbid agent could be evaluated by this method .
It is easy to detect resistant cells by this method .
It is more suitable for evaluation of anti-fungal agents .
20. Sader HS, Flamm RK, Jones RN (2013). Antimicrobial
activity of daptomycin tested against Gram-positive
pathogens collected in Europe, Latin America, and selected
countries in the Asia-Pacific Region. Diagn Microbiol Infect
Dis 75: 417-
