The document summarizes Kenya's efforts to institutionalize a One Health (OH) approach through the establishment of the Zoonotic Disease Unit (ZDU) in 2012. The ZDU is a structural office located between the ministries of human and animal health. It has 2 epidemiologists and support from other experts as needed. The ZDU aims to strengthen surveillance and control of zoonotic diseases, establish OH structures and partnerships, and conduct applied research. Some successes include joint surveillance and responses to zoonotic disease outbreaks. Challenges include limited resources, coordination between sectors, and operationalizing OH at local levels. Lessons learned include identifying region-specific OH drivers and taking a sustainable systems approach to implementation.