Using Probiotics to eradicate Corona Virus

1. Bio Tech GTX Probiotics level the playing field of bad
bacteria and the spread of the Covid virus
new proven test results?
“Important Warning: © Copyright in and to the Bio Tech GTX
product vests in and is owned by Green Worx Cleaning Solutions
(Pty) Limited. Any infringement of such copyright will be regarded
in a very serious light and immediate legal action will follow to
protect the rights of Green Worx Cleaning Solutions (Pty) Limited.”

2.  Green Worx Cleaning Solutions introduces a proven new age surface cleaning
hygiene sanitising probiotic bio-enzyme technology.
 That will keep all surfaces clean through improved hygiene methods – an
important step in avoiding getting sick and spreading germs and viruses.
 Bio Tech GTX Probiotic Surface Cleaner & Sanitiser is 99.9 % effective
against CORONA VIRUS, H1N1, common flu virus and SARS within 30
seconds, MRSA, E coli, and several other pathogens.

3. Some facts that are not clearly
understood and used incorrectly
• Sanitise - to make something completely clean
• Sanitiser - a substance used to make something clean and hygienic.
• Disinfect - clean (something), especially with a chemical, in order to destroy bacteria.
• Disinfectant - a chemical liquid that destroys bacteria
• Disinfectants are chemical agents applied to non-living objects in order to destroy bacteria,
viruses, fungi, mould or mildews living on the objects.
• Biocide - a substance that destroys living things, especially a pesticide, fungicide, or herbicide
The main difference between sanitizers and disinfectants is that sanitizers reduce the number of
germs on a surface whereas disinfectants chemically kill most of them.
The name hand "sanitizer" is a misnomer since its main germ-fighting ingredient is soap
Alcohol is a disinfectant of extreme short duration
First clean and then disinfect your surfaces – you cannot disinfect dirt

4. The SARS-CoV-2, the coronavirus
Bio Tech Probiotic
Surface Cleaner and Sanitiser

5. Bio Tech Probiotic Surface Cleaner &
Sanitising Spray
The enzyme protease breaks
down protein – thus breaking
down the envelope protein, the Membrane
protein, the Nucleocapsid protein
The enzyme lipase breaks
down the lipid membrane
Bio Tech GTX Bacillus Subtilis produces two important enzymes

6. How does Bio Tech GTX work
Mode of action: Enzymes
• Bio Tech GTX contains Bacillus Subtilis a probiotic which release significant
amounts of free enzymes, mostly proteases lipases and amylases
• Bacillus spores can operate as mini enzyme factories, delivering these
types of enzymes directly to the soiling source and without the issues of
sensitisation or inhalation
• Bacillus will continue to work until the available food source has been
used up and will then revert to the spore state and sit in the matrix until
more food comes along
• Bio Tech GTX Bacillus Probiotics based formulae can give long term
cleanliness and activity between product applications which no chemical
can deliver

7. Bio Tech GT Probiotic Surface
Sanitising Spray
Bio Enzymes are special proteins
that can break large molecules
into small molecules. Different
types of enzymes can break
down different nutrients: ...
protease enzymes break down
proteins into amino acids.
Lipase enzymes break down
lipids (fats and oils) into fatty
acids and glycerol.

8. Mode of action: Secondary metabolites
• We know that Bacillus strains / Probiotics are some of
the most active in terms of producing extracellular
metabolites that influence the growth of other
organisms
• We also know that Bacillus stains can produce surfactin, one of
the most potent emulsifying agents ever identified
• This has a function of emulsifying non aqueous substrates like
fats, oils and grease converting them to the ideal form for
biodegradation
• Targeted surfactin production in situ by Bacillus spores may
reduce the need for chemical surfactants within a formula.
Concentrated surfactin may also have significant membrane
disruption effects and may be a further mechanism for anti-
microbial effects

9. Mode of action: Competitive exclusion
• The simplest mechanism for their effect is simply “competitive exclusion”
• There is only a limited amount of food (or other limiting growth requirement) within any
environment
• Those organisms that reach the buffet first and grab the most food will squeeze out the
slower reacting organisms and starve them of food or other environmental factors and
eventually dominate the environment
• This is probably the simplest mechanism to explain to a layperson and is also welcomed by
regulators as there is no direct anti- microbial affect and no mediating metabolites that
need to be considered

10. Creating a ‘healthy microbiome’
How can Bio Tech GTX Probiotics maintain a healthy surface?
• Immediately after cleaning/spraying with Bio Tech GTX Probiotic Cleaner, a layer of good bacteria
is deposited on the treated surfaces
• The good bacteria immediately occupy all the available space, and compete for food and
water, preventing newly arriving pathogens from multiplying.

11. Of prime importance in cleaning and infection
control is the “Protection Time Factor”
 Studies show that most surface cleaning is inconsistent. Therefore, the
protection from disinfecting soaps and sanitizers of only minutes, compared
to the many hours of Bio Tech GTX Probiotic protection offering significant
advantages in cleaning and sanitizing control.
 Disinfectants stop working as soon as they have dried or evaporated from any
surface. Bio Tech GTX Probiotics keep working, cleaning and protecting
surfaces, for up to seven days after each application.
 However, since surfaces and skin are a dynamic environment that is
constantly being touched and are touching many different surfaces,
repetitively being contaminated, the Bio Tech GTX Probiotic Bio Enzymes
should be used frequently on all surfaces.

12. Green Worx new product introduction
Bio Tech GTX Probiotic Surface Cleaner & Sanitiser from Green Worx Cleaning Solutions
introduces new age sanitising, cleaning hygiene probiotic bio-enzyme technology.
Keeping surfaces clean through improved hygiene is one of the most important steps we can take to avoid
getting sick and spreading germs (harmful bacteria & viruses) to others. Many diseases and conditions are
spread by not cleaning surfaces and washing hands with soap and clean running water.
Bio Tech GTX Surface Cleaner & Sanitiser is unique in its formulation
Bacteria Counts : 10 times concentrate CFU 6 X 108 /ml
: Ready to Use CFU 6 X 107 /ml
Bacteria Type : Six strains of Bacillus consortium producing the following enzymes:
Protease – breaks down proteins (e.g. meat, excreted/secreted proteins) into amino acids
Lipase – breaks down fats/grease into fatty acids & glycerol. If not broken down, fats can go rancid & lead to
off-odours and blocked drains/fat grease traps.
Amylase – starch acts as a glue for dirt – amylases catalyse the break-down of starch into sugars which are
then further used as a food source by the bacillus
Cellulase – breaks down cellulosic material
Urease - catalyzes the hydrolysis of urea into break-down products.
Esterase - splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis. Esters
have characteristic odours most of which are pleasant/fruity, however can also include onion/garlic and
worse odours
Xylanase – help in breaking down plant cell walls.
What this means – the bacillus use the multitude of enzymes produced to break down the components
of malodour and staining to provide microbial cleaning at the smallest level of dirt/contamination.

13. Bio Tech GTX Probiotic Surface Sanitising
Spray
It is well know that Bacillus stains can produce surfactin, one of the most potent emulsifying agents ever
identified.
• This has a function of emulsifying non aqueous substrates like fats, oils and grease converting them to the
ideal form for biodegradation.
• Targeted surfactin production in situ by Bacillus spores may reduce the need for chemical surfactants
within a formula.
• Concentrated surfactin may also have significant membrane disruption effects and may be a further
mechanism for anti- microbial effects.
• The simplest mechanism for their effect is simply “competitive exclusion”. There is only a limited amount
of food (or other limiting growth requirement) within any environment. Those organisms that reach the
buffet first and grab the most food will squeeze out the slower reacting organisms and starve them of food
or other environmental factors and eventually dominate the environment.
• This is probably the simplest mechanism to explain to a layperson and is also welcomed by regulators
as there is no direct anti- microbial affect and no mediating metabolites that need to be considered

14. Bio Tech GTX Research and testing
results
All test results where obtained by applying:
 International Test Methods
 Laboratories with reputable International Accreditations – ISO 14001 and ISO
9001

15. Anti-microbial activity of Bio Tech GTX
Contact Time: 24hours
Organism challenged with Control sample Test sample % Kill (1)
S. aureus 2.0E+09 1.0E+02 99.9%
E. coli 2.3E+08 5.0E+03 99.9%
P. aeruginosa 3.0E+07 1.0E+02 99.9%
P. vulgaris 1.1E+08 1.0E+02 99.9%
E. aerogens 6.0E+08 4.2E+04 99.9%
E. faecalis 1.4E+08 1.1E+04 99.9%
A. faecalis 7.0E+08 1.0E+03 99.9%
C. albicans 2.1E+06 1.0E+01 99.9%
S. epidermidis 2.8E+06 5.0E+03 99.8%
M. luteus 9.0E+07 1.1E+04 99.9%
In addition to the superior cleaning ability of it has been proven that Bio Tech GTX is a superb anti – microbial that has a positive
kill of 99% on the following microorganisms. In a study Bio Tech GTX 1 at 1:4 dilution during a 24hours contact time showed:
Surviving microorganisms (cfu) and % Kill at 1:4 dilution and 24hours contact time 2
Study was conducted by Mirochem Specialised lab services – full study available on request

18. Sample
Identification Test Culture
No. of
colonies
recovered at
‘0’ hr [B]
No. of colonies
recovered at ’30
mins [A]
Reduction of
Microorganisms
[R]
1. Bio Tech GTX Probiotic
Floor cleaner
Methicillin-resistant
Staphylococcus aureus
(MRSA)
1.05 X 10^5 3.4 X 10^2 99.67%
Klebsiella pneumoniae 1.09 X 10^5 4.5 X 10^2 99.58%
Escherichia coli 1.15 X 10^5 4.9 X 10^2 99.57%
Pseudomonas
aeruginosa
1.11 X 10^5 5.4 X 10^2 99.51%
Antibacterial Activity Results for 30 mins contact time

19. Longevity of Bio Enzyme / Probiotics
In a recent comparison of a conventional standard hard surface chemical cleaning product vs. Bio Tech GTX a probiotic
cleaning / sanitizing product was tested in a controlled laboratory environment and in an uncontrolled “real world”
setting.
• The monitoring of adenosine tri-phosphate (ATP) as an indication of cleanliness and an ATP determination assay was
used to compare the products.
• The cleaning products were monitored over a scheduled time period to determine their long lasting efficacy in
reducing the ATP levels.
• The scope of this study was to determine the longevity of cleaning and decreasing levels of contamination.
• Bio Tech GTX10 at a dilution of 1:10 reduced ATP levels in the laboratory test and maintained these low levels for 168
hours (7 days). It was also effective in reducing and maintaining low ATP levels in 10 out of 10 of the various surface
areas tested for the same period of time.
• ATP was monitored over a time period, namely 1 hour, 3 hours, 5 hours, and 24 hours and 168 hours
The level of contamination was measured across different types of floor surfaces: Tearoom: Rough ceramic tile, Bathroom: Smooth ceramic tile, Kitchen:
Smooth ceramic tile, Hallway: Rough ceramic tile, Stairwell: Vinyl
Areas were divided in to high and low traffic.

20. Bio Tech GTX Probiotic Surface Cleaner &
Sanitiser: Longevity efficacy test vs conventional
chemical cleaning product

21. Bio Tech GTX Probiotic Surface Cleaner
& Sanitiser: Longevity efficacy test
% Contaminant Variance between Bio Tech GTX 10 (1:10) vs Product A
Hrs 1 Hrs 3 Hrs 5 Hrs 24 Hrs 72 Hrs 120 Hrs 168
14,0 2,5 -60,6 -22,7 -1,9 -25,2 -43,4
In Conclusion - from this study it can be seen that over
the various time periods Bio Tech GTX 10 FF diluted 1:10
displayed more superior cleaning efficacies than the
competitor for longer periods of time thus reducing the
cost of having to clean more often and reducing the time
and effort for cleaning staff to clean more often. Bio
Tech GTX 10 FF (1:10 dilution) remained effective on the
surfaces for 168 hours or 7 days.
Studies suggest that SARS-CoV-2 may remain
infectious on surfaces for hours to days
depending on the type of surface.

22. Bio Tech GTX Probiotic Surface Cleaner &
Sanitiser is -
✔ non-toxic
✔ non-irritating
✔ non-gaseous
✔ non-flammable
✔ non-pathogenic
✔ non-hazardous

23. Bio Tech GTX Probiotic Surface Cleaner & Sanitizer
Spray
is accredited by

24. Should you be using Bio Tech GTX Probiotics
or conventional chemical disinfectants? –
it makes you think?

25. Thank you for your attention
Need more information please contact Green Worx CS
Phone - +27 011 708 6626
Email – info@green-worxcs.co.za
Visit our website – www.green-worxcs.co.za