2. Who We Are?
An industrial biotech company situated in the CSIR Mining Innovation Centre,
with over 6 years already dedicated to R&D in conjunction with the Council for
Science and Industrial Research (CSIR) to develop sustainable biocides.
Based on the development of DECONT-X, a mild organic biocidal active ingredient,
BiODX products have gained popularity due to its non-corrosive properties and nontoxicity surrounding microbial decontamination.
Industrial Formulations
Food and Beverage
Health
Agricultural
Copyright BiODX 2013 All Rights Reserved

3. The Technology
• BiODX’s research was based upon a principal which assumed that triggering
an action on sensory parameters of citrus would yield high dosages of
biological yield from the target gene.
• BiODX’s innovative processes use the natural enzyme reaction occurring from
the time of picking the fruit, where degradation of the antimicrobial component
starts as the fruit converts all resources (sugars, bioflavonoids, antimicrobials
etc.) in order to stay alive.
• Innovation is also seen in the formation of two active compounds that do not
exist in the raw fruit; these two compounds are formed by virtue of the digestion
process that converts citric acid and vitamins.
• Formulating preservative into the juice is a stoichiometric reaction. With
reference to the formation of vitamin C, a citrus oxidation-reduction system has
electron donating and electron accepting properties.
Copyright BiODX 2013 All Rights Reserved

4. Active Citrus Extract
GC (gas chromatography) analysis identified active peak in citrus extract.
Bioflavonoids which is a main component of citrus has previously been reported as
having a growth inhibitory effect on bacteria such as colibacillus, Staphylococcus
aureus or methicillin-resistant Staphylococcus aureus.
Copyright BiODX 2013 All Rights Reserved

5. Stabilised Citrus Extract
GC (gas chromatography) analysis of DECONT-X showing activity compounds and
newly formed sub compounds post digestion.
The reaction between the citrus extract and QAC is a stoichiometric reaction
causing an ion exchange between the two compounds allowing the big molecule
(citrus extract) to digest certain sugars and other elements into newly formed
compounds.
Copyright BiODX 2013 All Rights Reserved

6. Regulatory Compliance
Originating in response to pollution laws in the USA, organic chemists use all the tools
and training of traditional chemistry to create non-hazardous chemicals and processes
that are "benign by design".
SABS SPECIFICATION
NUMBER
DESCRIPTION
FDA & EPA (United States of
America) Regulation
SANS 636
Disinfectants based on Quaternary
Ammonium Compounds
400ppm
SANS 1615
Detergent-Disinfectants based on
Glutaraldehyde
400ppm
SANS 1828
Cleaning Chemicals for the Food
Industry
400ppm
SANS 1853
Disinfectants and Detergent
Disinfectants for use in the Food
Industry
400ppm
SABS MARK
Quality Management System
ISO Accredited
KOSHER
Certification
Eco-Friendly Disinfectant
HALAAL
Pending
Copyright BiODX 2013 All Rights Reserved

7. Product Comparative
Safety Matrix
Organic chemistry describes a way of carrying out industrial activities from start (design)
to finish (production), it is driven by overwhelming consumer demands, regulatory and
economic factors.
Ingredient
Toxicity
Environmental
Concerns
Handling
Sensory
Effect
Corrosion
Natural
Alcohols
N
N
Y
N
N
N
Aldehydes
Y
N
N
N
N
N
Paracetic Acid
Y
N
Y
Y
Y
N
Phenols
Y
Y
N
Y
N
N
Hypochlorite
Y
Y
Y
N
Y
N
Hydrogen
Peroxide
N
N
Y
N
Y
N
DECONT-X
N
Genopole
N
N
N
N
Copyright BiODX 2013 All Rights Reserved

8. BiODX Products VS.
Conventional Chemicals
Bring innovation to your customers to have differentiation from competition.
Conventional
60% Natural
80% Natural
Chlorine,
Heavy Metals,
Solvents,
Paracetic Acid,
Caustic Soda,
Aldehydes
Pollution, effluent fines, corrosive,
toxic waste, kills enzymes,
damage to the environment
Earth Friendly, biodegradable,
non-corrosive, non-toxic,
harmless to the environment
Copyright BiODX 2013 All Rights Reserved

9. About the Product
Properties of DECONT-X:
• All South African
• Earth friendly and fully biodegradable.
• Bactericidal, fungicidal and virucidal.
• Pleasant characteristic fragrance due to citrus content.
• Has characteristic detergent properties.
• Unaffected by ammonia & nitrates.
• Not reactive with organic matter (no THM).
• Fast kill kinetics on both sessile and planktonic bacteria.
Copyright BiODX 2013 All Rights Reserved

10. Safety Characteristics
•
Non-irritant at normal use levels
•
Non-carcinogenic
SS
•
Non-mutagenic
•
Non-embryotoxic
•
Non-teratogenic
•
Contains no alcohol, formaldehydes or other harmful chemicals.
•
Chloride Free - does not generate any induced chemical corrosion regardless
the residence or contact time.
Copyright BiODX 2013 All Rights Reserved

11. Compatibility, Solubility
& Stability
•
DECONT-X is miscible with water and polar organic solvents.
•
Stable in acid and alkaline pH environments from pH 2 up to pH 11.
•
Compatible with a wide range of additives and auxiliary products including
surfactants, flocculants, dispersants, corrosion inhibitors and other biocides.
•
In common with other non-oxidising biocides, strong reducing and oxidising
agents should be avoided.
•
A potent antimicrobial with broad spectrum activity against Gram negative and
Gram positive bacteria, SRB’s, APB’s, anaerobes, yeasts and fungi.
•
Possible anion replacement for stabilization under acid condition.
Copyright BiODX 2013 All Rights Reserved

12. Product Performance
The “intelligence” of DECONT-X kills 99.9 % of pathogenic micro-organisms &
MIC’s (microbials influencing corrosion).
•
Escherichia coli sp.
•
Pseudomonas sp.
•
Staphylococcus sp. + Streptococcus sp.
•
Listeria sp.
•
Effective against legionella at as low as 30 ppm.
•
Black, green & brown moulds (Stachybotrys)
•
SRB’s, ARB’s & Anaerobes (MIC’s)
•
Remarkably effective against Pseudomonas, Stachybotrys and MIC’s.
Copyright BiODX 2013 All Rights Reserved

13. Performance
on Hard Surfaces
Microbial Count
The following results show the microbial control obtained at the beginning,
during and completion of a 3 month trial.
Copyright BiODX 2013 All Rights Reserved

14. DECONT-X Benchmarking
VS. Conventional Chemicals
Amount of Bacteria cfu/mL
The number of internal failures related to MIC and chemical corrosion issues
degrease by 80% with the use of DECONT-X.
Copyright BiODX 2013 All Rights Reserved

15. Comparative MIC Agent
Corrosion Rates
DECONT-X allows long residence time without generating any induced chemical
corrosion and offers a technical alternate to reduce dependency on Glut and THPS.
Autoclave Corrosion Results on 1018 Steel
MPY
Copyright BiODX 2013 All Rights Reserved

16. Markets for DECONT-X
•
Oil / Petroleum (fuel additive)
•
Fracking
•
Pulp / Paper Mill Industry
•
Water Treatment / Cooling Towers
•
Biofilm Control
•
Disinfectant Formulation
•
Preservation / Coatings / Paint
•
Printing Chemicals / Ink
BiODX have positioned DECONT-X selectively in these markets where a
sustainable biocide will have a competitive advantage due to end-user concerns
related to the environment, product performance and toxicity.
Copyright BiODX 2013 All Rights Reserved

17. Toxicity &
Corrosion Matrix
Conventional chemicals as demonstrated pose harmful risks to the labour force
promoting absenteeism and corrosive properties leads to equipment damage
resulting in huge financial losses.
Conventional
Chemicals
Max
Toxicity /
Corrosion
Sustainable
Chemicals
Paracetic Acid
Phenols
Hypochlorite
Hydrogen Peroxide
Aldehydes
Health /
Financial
Losses
Min
Natural
Natural
Copyright BiODX 2013 All Rights Reserved

18. Product Advantages
One of many comparative examples of sustainable VS. conventional chemicals
as applicable to cooling towers and there affect on water chemistry.
DECONT-X
CHLORINE
Unaffected by ammonia & nitrates
Affected by ammonia and nitrates
Non-toxic, no health & safety concerns
Major health & safety concerns
pH Neutral
Perceived as a protein/food - no resistance
Affects water pH
Bacterial strains can resist it
Non-corrosive
Highly corrosive
Outstandingly effective against biofilm
No efficacy against biofilm
No reaction to organic matter
Produces trihalomethanes / carcinogens
Stand alone product
Require extra chemicals
Stable at 5°c to 112°c
Unstable
At 15-25 ppm (in water) is cheaper than chlorine
Higher costs
Copyright BiODX 2013 All Rights Reserved

19. Total Bacteria Control in
Cooling Tower Water
Microbial Load (cfu/cm2)
The water sample was taken directly from a cooling tower that had undergone
standard treatment of a two ton shock treatment of chlorine added every two weeks.
The control did not get treated with any additional biocide during the 72 hours.
- Control
- DECONT-X
Time in Hours
Copyright BiODX 2013 All Rights Reserved

20. Bacterial Resistance
Bacteria learn to acquire immunity to conventional chemicals, which means:
• Stronger chemical concentrations
• Increased chemical costs
• Increased water / rinse costs
• Increased electricity / heating costs
• Increased process costs
• Increased labour / absenteeism costs
Bacteria do not identify DECONT-X as a poison and therefore do not imitate
development of resistance.
Copyright BiODX 2013 All Rights Reserved

21. The Knock-On Effect slide 1 of 2
Savings to a companies bottom-line are accrued by using sustainable chemicals, this
is the responsibility of the CEO as it influences many knock-on effects.
Product Cost
The Knock-on Effect
Conventional
Chemicals
Sustainable
Products
Water
Electricity
Effluent
Corrosion
Absenteeism
Sustainable Product VS. Conventional Chemicals
Copyright BiODX 2013 All Rights Reserved

22. The Hidden Costs to the
Knock-On Effect
slide 2 of 2
Conventional chemicals require heat, rinsing, water chemistry and affect the pH,
this mixture of chemicals is harmful to the environment, human health and is
highly corrosive thus affecting the companies bottom-line!
Cost
Conventional
DECONT-X
Product Cost
€ 3.00
€ 7.50
Electricity
€ 0.40
€ 0.00
Water
€ 0.20
€ 0.00
Effluent
€ 1.00
€ 0.00
Stabilising Chemicals
€ 2.50
€ 0.00
Corrosion
€ 0.50
€ 0.00
Absenteeism
€ 2.00
€ 0.00
Total
€ 9.60
€ 7.50
Copyright BiODX 2013 All Rights Reserved

23. Are you Green or
Green-washed?
Become an authority!
Marketing green & overcoming green-washing.
Copyright BiODX 2013 All Rights Reserved

24. Drop the Sentiment!
Time for green sentiment to get out of the boardroom!
Bunnies go home!
Tree huggers out!
Green business is about making profits greener – with Industrial Ecology
Copyright BiODX 2013 All Rights Reserved

25. What are we really
selling?
An economy based on the intelligence of natural systems!
Copyright BiODX 2013 All Rights Reserved

26. Contact Us
Tel: 011 358-0052
info@biodx.co.za
www.biodx.co.za
CSIR – Mining Innovation Centre
Building 2
Cnr. Carlow & Rustenburg Rd
Melville, Johannesburg
South Africa
Graham Poultney
Tel: 011 358-0052
Cell: 082 447 3467
graham@biodx.co.za
www.biodx.co.za
Copyright BiODX 2013 All Rights Reserved