By: Crystal Hamelburg and Tomasz Perlinski
Organic carrots should exhibit a higher quantity of
microbial growth than their conventional counterparts,
even after the ...
• There were two main objectives of this study:
• The first objective involved testing the efficacy
of three different san...
Media Preparation
• 40 g of Tryptic Soy Agar was weighed out on a
balance and slowly added to 1.0 L of distilled
water, wh...
Sample Preparation
Carrots were washed with warm
tap water and dawn dish soap
A borer, forceps, and scissors
were autoclav...
Cutting Board Preparation
A used cutting board was obtained from Texas
Roadhouse, cut into four roughly equal
boards, and ...
Disinfectants
Control: Sterile
Distilled Water
1% Bleach Solution 50% Diluted Eco-San Lime Essential Oil
(1 mL Lime + 2 mL...
Pre and Post experiment procedures and testing
• Four separate sterile cotton swabs were used to test each cutting board p...
Application of Escherichia coli and Disinfectants
A McFarland standard was used to prepare the E.Coli cells
0.5 mL of E.co...
Incorporation of carrot core samples and
consequent plating on TSA plates
Giant Organic
Shoprite
Organic
Weis Organic
Gian...
Colony Quantification
Control Bleach Eco-San Lime Essential Oil
Gram Stains
Gram stains were performed on the E.coli
Bacteria after they were given a 24 hour incubation
period.
The TSA p...
Figure 5: Sample gram stains from colony forming carrot samples.
Photographs of gram stains, showing E. coli (gram negativ...
Table 1: Colony Quantification of Experiment I. The number of colonies
that formed from the carrot swabs after the 24 hour...
Sanitizer Effectiveness
0
5 5
2
0
1
2
3
4
5
Control Bleach EcoSan Lime
ScaleofEffectiveness
Figure 1: Sanitizer Effectiven...
Pre and Post Experiment Testing
1
0 0 0
Control Bleach EcoSan Lime
Figure 2: Cutting Board Cleanliness Before the start of...
Organic vs. Conventionally Grown Carrot Cores
Figure 4: Microbial Growth Comparison between
Organic and Conventional Carro...
Eco-San was found
to be the most
effective.
Bleach was also
found to be the
most effective.
Lime essential oil
was found t...
Overall, it was observed that organic carrots exhibited more microbial growth.
Conventional carrots are often associated w...
Typically, the use of the external portion of the carrots were utilized for
experimental procedures in the literature that...
A comparison between organic and conventionally grown carrots prepared on cutting boards sanitized in various ways
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A comparison between organic and conventionally grown carrots prepared on cutting boards sanitized in various ways

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Hypothesis: Organic carrots should exhibit a higher quantity of microbial growth than their conventional counterparts, even after the application of various sanitizers.

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A comparison between organic and conventionally grown carrots prepared on cutting boards sanitized in various ways

  1. 1. By: Crystal Hamelburg and Tomasz Perlinski
  2. 2. Organic carrots should exhibit a higher quantity of microbial growth than their conventional counterparts, even after the application of various sanitizers.
  3. 3. • There were two main objectives of this study: • The first objective involved testing the efficacy of three different sanitizers used to clean cutting boards purposely contaminated with Escherichia coli. • The second objective was to compare the quantity of E.Coli contamination present on organic and conventionally grown carrots prepared on the cutting boards after the application of the disinfectants.
  4. 4. Media Preparation • 40 g of Tryptic Soy Agar was weighed out on a balance and slowly added to 1.0 L of distilled water, while the flask was stirred with a stir bar (directions obtained directly from the bottle) • The media was placed into the autoclave on a Liquid 9 setting for 45 minutes at 121°C • 56 plates were poured for the two trials conducted
  5. 5. Sample Preparation Carrots were washed with warm tap water and dawn dish soap A borer, forceps, and scissors were autoclaved and used to obtain the carrot cores Carrot cores were obtained under a sterile hood and placed into sterile weigh boats
  6. 6. Cutting Board Preparation A used cutting board was obtained from Texas Roadhouse, cut into four roughly equal boards, and sterilized under UV light for 2 hours. Each board was divided into 6 sections and labeled the “control, bleach, Eco-San, and lime.”
  7. 7. Disinfectants Control: Sterile Distilled Water 1% Bleach Solution 50% Diluted Eco-San Lime Essential Oil (1 mL Lime + 2 mL dH2O)(10 mL dH2O+10 mL Eco San)
  8. 8. Pre and Post experiment procedures and testing • Four separate sterile cotton swabs were used to test each cutting board prior to the application of any E.coli to ensure that they were free from contamination that would skew the results. • Four Additional samples were collected after the application of each disinfectant.
  9. 9. Application of Escherichia coli and Disinfectants A McFarland standard was used to prepare the E.Coli cells 0.5 mL of E.coli was transferred using a sterile pipette to each section of the four cutting boards The bacteria was spread all over each section of the cutting board using a sterile cotton swab The bacteria were given 10 minutes to dry before 0.5 mL of each respective sanitizer was applied to each respective section
  10. 10. Incorporation of carrot core samples and consequent plating on TSA plates Giant Organic Shoprite Organic Weis Organic Giant Conventional Shoprite Conventional Weis Conventional • Carrot cores were spread around about an inch forward and then back again with forceps. • The Bunsen burner was used to sterilize the forceps after each carrot core application.
  11. 11. Colony Quantification Control Bleach Eco-San Lime Essential Oil
  12. 12. Gram Stains Gram stains were performed on the E.coli Bacteria after they were given a 24 hour incubation period. The TSA plates that showed growth were tested using the standard gram stain procedure. This allowed the quantification of any sort of contamination.
  13. 13. Figure 5: Sample gram stains from colony forming carrot samples. Photographs of gram stains, showing E. coli (gram negative) colonies that formed from carrot core swabs. A – Sample gram stain of carrot swab used on control (dH2O) cutting board; B - Bleach treated cutting board, C – Eco-San treated cutting board, and D – Lime treated cutting board. Gram Stains
  14. 14. Table 1: Colony Quantification of Experiment I. The number of colonies that formed from the carrot swabs after the 24 hour incubation period (standard conditions). *Using a streak plate method and counted using naked eye. Table 2: Colony Quantification of Experiment II. The number of colonies that formed from the carrot swabs after the 24 hour incubation period (standard conditions). *Using a streak plate method and counted using naked eye. Colony Counts Experiment I Carrots Control (dH2O) Bleach Eco-San Lime Giant Organic TNTC 0 0 5 Giant Conventional TNTC 0 0 TNTC Shoprite Organic 16 0 0 TNTC Shoprite Conventional TNTC 0 0 TNTC Weis Organic 0 0 0 TNTC Weis Conventional 2 0 0 TNTC Experiment II Carrots Control (dH2O) Bleach Eco-San Lime Giant Organic TNTC 2 0 0 Giant Conventional TNTC 0 0 0 Shoprite Organic 83 6 0 0 Shoprite Conventional TNTC 0 0 0 Weis Organic 28 0 0 42 Weis Conventional TNTC 0 1 0
  15. 15. Sanitizer Effectiveness 0 5 5 2 0 1 2 3 4 5 Control Bleach EcoSan Lime ScaleofEffectiveness Figure 1: Sanitizer Effectiveness Scale. The effectiveness of sanitizers used compared to the control, using a 1-5 scale. (0 = Not effective, 5 = Very effective) *Effectiveness refers to the number of colonies formed, 0-20 CFU’s is considered very effective. The most effective sanitizers were Bleach and Eco- San. The lime’s effectiveness varied between experiments, but was not very effective in either case.
  16. 16. Pre and Post Experiment Testing 1 0 0 0 Control Bleach EcoSan Lime Figure 2: Cutting Board Cleanliness Before the start of the experiment. A sample of each cutting board was plated on TSA to evaluate the effectiveness of cutting board sterilization under the UV light in a laminar flow biological hood for approximately 2 hours. Any colony growth after a 24 incubation period was noted. +330 4 0 11 Control Bleach EcoSan Lime Figure 3: Cutting Board Cleanliness 10 minutes after applying Sanitizers. A sample of each cutting board was plated on TSA to evaluate the effectiveness of the sanitizers before applying the carrot samples. Any colony growth after a 24 incubation period was noted.
  17. 17. Organic vs. Conventionally Grown Carrot Cores Figure 4: Microbial Growth Comparison between Organic and Conventional Carrots. The number of each carrot category that had colony formation was summed together to determine the total number of organic carrot growth to conventional carrot growth out of a possible 24 samples. For example, every time there was growth on a TSA plate it was counted as “1”.
  18. 18. Eco-San was found to be the most effective. Bleach was also found to be the most effective. Lime essential oil was found to be the least effective. Effectiveness of Sanitizers The results of this experiment have shown that plastic cutting boards can be effectively cleaned with the use of various sanitizers that may help to prevent cross contamination.
  19. 19. Overall, it was observed that organic carrots exhibited more microbial growth. Conventional carrots are often associated with the application of chemical fertilizers, genetic modification, hormones, pesticides, and residues in food. Microbial growth comparison between organic and conventional produce
  20. 20. Typically, the use of the external portion of the carrots were utilized for experimental procedures in the literature that was explored. However, our experiment used aseptic carrot cores, but was still consistent with theories that stated organic microbial growth would be higher than conventional carrot growth, despite the differences in methods. Literature Comparison

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