Lab safty rule for managing statigies

1. Biohazard and Biorisk assessment, with
biological/chemical/animal work risk assessment
• presented by: IQRA AKRAM (2022-ag-2062)
IMAMA NASIR (2022-ag-1179)
TAYYABA WALI (2022-ag-1981)
SARA REHMAN(2022-ag-1993)
SANA WAHEED(2022-ag-1987)
RIDA FATIMA(2017-ag-8424)
SAMAN TAHIRA(2021-ag-1780)
ARFA ZULFIQAR(2021-ag-1802)

2. Introduction to Biohazard and Biorisk assessment
Biohazard
• A biohazard refers to any biological substance that poses a threat
to human health or the environment.These substances can
include microorganisms (such as bacteria, viruses, fungi, and
parasites), toxins produced by living organisms, and biological
materials that may contain these harmful agents.
• Biorisk Assessment
• Biorisk assessment is a systematic process that evaluates and
manages potential risks associated with biological agents and
materials.it includes hazard identification,risk assessment and
implementation of measures to control and mitigate risks.
• Purpose of Biorisk assessment is to ensure safe handling of
biological materials and effective response to biohazard incidens

3. Significance of risk assessment in scientific work
• Risk assessment is crucial in scientific work as it
systematically identifies and evaluates potential
hazards, enabling researchers to mitigate risks
and ensure the safety of both the experimenters
and the environment.
• Additionally, it enhances the reliability of
experimental results by minimizing unforeseen
complications, thereby contributing to the overall
integrity and credibility of scientific research.

4. Biological work concideration
• Biological material that have a known
risk to people and environment:
• Microorganisms(bacteria,viruses and
protists)
• human blood tissues and body
fluids(human blood,serum,tissues and
faecal specimen). this can have cell lines
of human or primate origin that are
known to have infectious viral agents.

5. Necessity of Risk Assessment
• Biological hazard risk assessment is used to alert
the staff to the hazards of working with infectious
agent and to the need for developing proficieny in
the use of selected safe practices and
containment equipment.
• Successful control of hazards in lab also protect
persons not directly associated with the lab, such
as other occupants of the same building, and the
public.

6. Chemical Work Considerations
• Common Hazards
1. Chemical spills and leaks
2. Toxic fumes and gases
3. Fire and explosions
4. Exposure to hazardous
substances

7. Necessity of Risk Assessment
• Steps in Risk Assessment
1. Identify hazards
2. Assess the risks
3. Implement control measures
4. Monitor and review
5. Legal and Ethical Obligations
6. Emphasize the legal requirements and ethical
responsibility of employers to conduct risk
assessments.
• Benefits
a. Improved safety culture
b. Reduced accidents and injuries
c. Compliance with regulations

8. Animal work consideration:
Animal work risk assessment:
Risk assessment is a crucial aspect of working with animals in a laboratory
setting to ensure the safety of both the researchers and the animals. Here are some
key considerations for conducting a risk assessment:
Physical Risks: Identify potential physical risks associated with handling animals,
such as bites, scratches, or injuries from larger animals. Implement appropriate
safety measures, such as using personal protective equipment (PPE) like gloves and
safety glasses.
Zoonotic Diseases: Assess the risk of zoonotic diseases (diseases that can be
transmitted between animals and humans). Follow recommended vaccination
protocols for relevant diseases.
Implement good hygiene practices, including handwashing, to reduce the risk
of disease transmission.

9. Necessity of Risk Assessment

10. Steps involved in risk assessment

11. Stakeholder involvement

12. Environmental Safety Procedures
• The environment health and safety center has implemented a comprehensive for the
management of hazardous materials
• Microbiological, Chemical laboratories and procedures involving microorganism and
chemicals pose special safety problems so the following policy and procedures must be
followed rigorously.
• Risk
• Warning signs
• Separate areas
• Protective clothing
• Safety Cabinent
• Decontamination
• Levels of containment

13. Cont...
• Risk: Pathogens and chemical should be handled carefully based on the biorisk they pose to staff,
general public and animal outside the laboratory.
• Warning signs: A dangerous hazard must be indicated by standard biological warning signs giving the
type and degree of risk.
• Separate areas: separate areas should be set aside for the preparation of media, holding of the
material and sterilization. Animal rooms must be segregated from laboratory and should separated
infected from non infected animals.
• Protection clothing
• Safety Cabinent: Laminar flow and
• Biosafety cabinent
• Decontamination: Sterilization, Disinfection
• Waste disposal
• Level of contaiment

14. Personnel Training and Continuous Education

15. Adapting to Biosafety Challenges through
Continuous Education

17. communication strategies and coordination in
emergency

18. Cont...