2. Introductionof biosafety and security
• Laboratory Biosafety: containment principles, technologies,
and practices implemented to prevent unintentional exposure
to pathogens and toxins, or their unintentional release
3. Introductionof biosafety and security
• Laboratory Biosecurity: protection, control, and
accountability for valuable biological materials within
laboratories, in order to prevent their unauthorized access,
loss, theft, misuse, diversion, or intentional release
4. Introductionof biosafety and security
• Biologic Terrorism: Use of biologic agents or toxins (e.g.,
pathogenic organisms that affect humans, animals, or plants)
for terrorist purposes.
• Biohazard: A biological agent or a condition that constitutes a
threat to humans, especially in biological work, research, or
experimentation. The potential danger, risk, or harm from
exposure to such an agent or condition.
Viruses, bacteria, fungi, and parasites and their toxins.
Blood and body fluids, as well as tissues from humans and
animals.
Transformed cell lines and certain types of nucleic acids.
A potential hazard to humans, animals or the environment
caused by a biological organism, or by material produced by
such an organism
5. Introductionof biosafety and security
• Risk: A measure of the potential loss of a specific biologic agent of
concern, based on the probability of occurrence of an adversary
event, effectiveness of protection, and consequence of loss.
• Risk Assessment: An analysis of the probability and the
consequences of loss, theft and potential misuse of pathogens and
toxins.
• Biomedical Waste: Discarded biological material from teaching,
clinical and research laboratories, and operations. Biomedical waste
includes but is not limited to animal waste, biological laboratory
waste, human anatomical waste, human blood and body fluid waste
and sharps.
6. Biological safety objectives
1. Protective workers from disease resulting from
dangerous biological agents.
2. Maintain a safe working environment .
3. Prevent the spread of biological contaminates
outside site the work .
4. The control of biological contaminants through
management and risk assessment in the
workplace
7. RULES OF BIOSAFETY
• 1.) supervisors must properly train their employees before
permitting them to conduct biohazardous work.
• 2.) know and understand the biology and infectious
potential of biohazardous agents you handle.
• 3.) handle all biological material (tissues and body fluids)
recognized to be capable of potentially harboring
biohazardous organisms or agents as if the infectious
agent is present.
• 4.) always use good standards of microbiological practice
in handling biohazardous agents or materials or any
microorganism.
8. RULES OF BIOSAFETY
• 5.) use the biosafety level containment and practices specified
for the infectious agent by the centers for disease control and
prevention, atlanta, georgia.
• 6.) do not work with a microorganism or agent if the required
level of physical containment designated for the biohazardous
material being used is not available .
• 7.) each worker handling biohazardous material is responsible
for following safety rules to prevent injury to self and others.
• 8.) vigilance and monitoring of biosafety practices are essential
in any biosafety program.
• 9.) assume that accidents will occur and plan for safely
managing those events when handling biohazardous materials
and agents.
9. RULES OF BIOSAFETY
• 10.) report all accidents or incidents to your supervisor
immediately.
• 11.) use disinfectants or sterilants with proven efficacy against
the specific biohazardous agent(s) you are using.
• 12.) never permit biohazardous materials to leave the
laboratory unsterilized unless being transported to another
laboratory for additional work and packaged according to
appropriate regulations.
