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Endogenous and Exogenous Fluorescence Skin
Cancer Diagnostics for Clinical Applications
Abstract—A short review will be presented of the recent clinical achievements in the field of
skin autofluorescence and exogenous fluorescence tumor detection. Photosensitizers used for

2. exogenous photodetection of cutaneous lesionswill be discussed fromthe point of view of their
photodynamic diagnostic properties and their advantages and drawbacks associated with clinical
applications. A survey on various results of detection and differentiation of the fluorescent data
observed from malignant cutaneous lesions will be summarized and an optimization of the skin
cancer detection techniques based on fluorescence diagnostics will be analyzed and discussed. A
short presentation will be given of own experimental results and clinical experience acquired in
the past decade in the autofluorescence diagnostics of different benign, dysplastic, and malignant
skin neoplasia. The origins of the fluorescence spectra, their peculiarities, the feasibility of
clinical tumor detection, and differentiation needs will also be discussed.
Existing method:

3. combined usage of exogenous fluorescent compounds—for diagnostics and therapy—is
appropriate when one has preliminary information about the cutaneous lesion treated—a
malignancy proved by a previous clinical dermatological examination.
Proposed method

4. The most frequently used separation algorithm proposed by different research groups is related
to differences in the fluorescent intensity of the lesions and the surrounding normal skin
Merits
Complexity is high.
Demerits
Complexity is low.
Results

5. EEM of normal skin AF.
Normal skin AF spectra at different excitation wavelengths (from 270 to 500 nm) and
identification of the major endogenous fluorescence compounds observed, using knowledge
about their excitation and emission maxima