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1. Media preparation
2. Explant selection
3. Establishment of explant in media
4. Callus development
5. Plantlet development
6. Hardening or acclimatization
7. Open field planting
Lecture 12: Nucellus and endosperm culture
Course Code : HRT 552
Course Title : BIOTECHNOLOGY OF
HORTICULTURAL CROPs
2) Explant:-
Totipotent somatic cell are used.
Immature inflorescence and Scutellar tissue of
immature seeds are used for the research. Ex:-
Triticum aestivum .
Epidermis, Procambial tissue are also produced
somatic embryo.
THANKS 

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Nucellus and endosperm culture

  • 1. 1. Media preparation 2. Explant selection 3. Establishment of explant in media 4. Callus development 5. Plantlet development 6. Hardening or acclimatization 7. Open field planting Lecture 12: Nucellus and endosperm culture Course Code : HRT 552 Course Title : BIOTECHNOLOGY OF HORTICULTURAL CROPs
  • 2. 2) Explant:- Totipotent somatic cell are used. Immature inflorescence and Scutellar tissue of immature seeds are used for the research. Ex:- Triticum aestivum . Epidermis, Procambial tissue are also produced somatic embryo.
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