GBSN - Biochemistry (Unit 2) Basic concept of organic chemistry
Nucellus and endosperm culture
1. 1. Media preparation
2. Explant selection
3. Establishment of explant in media
4. Callus development
5. Plantlet development
6. Hardening or acclimatization
7. Open field planting
Lecture 12: Nucellus and endosperm culture
Course Code : HRT 552
Course Title : BIOTECHNOLOGY OF
HORTICULTURAL CROPs
2. 2) Explant:-
Totipotent somatic cell are used.
Immature inflorescence and Scutellar tissue of
immature seeds are used for the research. Ex:-
Triticum aestivum .
Epidermis, Procambial tissue are also produced
somatic embryo.