slides on reductive biosynthesis

1. Reductive Biosynthesis
It is defined as;
lAnabolic pathway that require hydride ion to
reduce carbon atom in metabolic intermediates.
lIt is synthesis of large molecules from smaller
ones that are used in formation of cellular
components.
1.E.g. Proteins from amino acids
2.DNA from nucleic acid
3.Lipids ,vitamins, glycogen etc.

2. Reducing agents
• Hydride ion is given by reducing agents that
are different from laboratories ones.
• They are NADH,FADH2,FMNH2,NADPH.
• They are found in redox pair ant get
deoxidized in other reactions.
• In reductive biosynthesis, only NADPH is
discussed because other ones are used in
catabolic processes.

3. NADPH
• Used in HMP,PPP.
• Fatty acid synthesis.
• Gluconeogenesis.
• Protein synthesis.

4. Fats synthesis
• Cholesterol is taken by diet and produced in
liver's cytosol, 800 mg per day.
• Palmitic acid is primary one.
• At each step elongation of 2 carbon atom is
done.

5. Pathway integration
• Other pathways are integrated which are
a.Protein degradation
b.Carbohydrate metabolism

6. Synthesis
• Acetyl CoA and CO2 reacts to form
malonylCoA i.e. Intermediate.