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Nano-coating , Nano-treatments
novel approaches to
Extend the post harvest life
of Horticultural produce
Aisha Kolhar
UHS15PGM620
Dept of Post Harvest Tech
Seminar I
on
7
Properties of Nano Particles
Case Studies
Conclusion
Mechanism of Nano Particles
History of nanotechnology
Introduction to nanotechnology
Nanotechnology ?...
“Nanotechnology is the art and
science of manipulating matter at
nanoscale”
The design, characterization, production and
application of structure, device and system by
controlling shape and size at nanoscale
British standard institution, 2005
8
‘Nano’- Greek word means ‘Dwarf’
 1 nm = one billionth (10ˉ⁹) of metre
 Size range between 0.1 and 100 nm
9
10
“There’s Plenty of Room at the Bottom”
- at American Physical Society meeting at the California
Institute of Technology on Dec-29, 1959.
“Adaptability to manipulate, control, assemble, produce
and manufacture things at atomic precision” 11
Father of nano technology
Nobel Laureate-1965
Richard Feynman, Physicist
Norio Taniguchi,
Professor - coined the term
“Nanotechnology” (1974)
“Nano-technology’’ - Processing, separation, consolidation and
deformation of materials by one atom or by one molecule.
12
~ 2000 Years
Ago
Sulfide nanocrystals used by Greeks and Romans to dye hairs
~ 1000 Years
Ago
Gold nanoparticles of different sizes used to produce different colors in
stained glass windows
1959 “There is plenty of room at the bottom” by R. Feynman
1974 Taniguchi uses the term nanotechnology for the first time
1981 IBM develops Scanning Tunneling Microscope
1985 “Buckyball” - Scientists at Rice University and University of Sussex discover
C60
1986 • “Engines of Creation” - First book on nanotechnology by K. Eric Drexler.
• Atomic Force Microscope invented by Binnig, Quate and Gerbe
1989 IBM logo made with individual atoms
1991 Carbon nanotube discovered by S. Iijima
1999 “Nanomedicine” – 1st nanomedicine book by R. Freitas
2000 “National Nanotechnology Initiative” launched
(British Standards Institution, 2005)
Time Line of Nanotechnology
13
14
Properties of nano particles
15
 Nano-scale materials show unusual physical, chemical and biological
properties. (Li et al., 2001)
 Nanoparticles have large surface to volume ratio (Kumar et al., 2010)
Magical changes takes place at Nano level
Physical Surface area, conductivity, charge capacity
Chemical Reactivity
Mechanical strength
Things behave differently in nano-world
 Carbon in the form of graphite (i.e. pencil lead)
is soft, at the nano-scale, can be stronger than
steel and is six times lighter
 Nano-scale copper is a highly elastic
metal at room temperature, stretching
to 50 times its original length without breaking
 Shiny orange yellow Gold changes its colour to
brownish black on reducing the size
16
(Royal Society and Royal Academy of Engineering, 2004)
METHODS OF NANOPARTICLE PRODUCTION
17
(Royal Society and Royal Academy of Engineering, 2004)
TOP DOWN APPROACH
18
(Royal Society and Royal Academy of Engineering, 2004)
19
BOTTOM UPAPPORAOCH
Dendrimers
Quantumdots
Nanosensors
FullerenesCarbon Nanotubes
Nano Chips
C60 Cadmium selinade
20
Tools of Nanotechnology
21
Why we need Nanotechnology ???
To enhance the efficiency of available post harvest technologies
To reduce the economics of additives.
Ethylene biosynthesis inhibitors – AVG,AOA : Expensive and
Phytotoxic
Many chemicals which we are using may be soon banned due to
their environmental impact
22
23
24
Case study 1
25
26
Fig.1 Changes in vase water uptake over time of cut gerbera cv. Ruikou
flowers following NS pulse treatments
27
Fig.2 Changes in relative fresh weight (B) over time of cut gerbera cv. Ruikou
flowers following NS pulse treatments
28
Fig. 3 Changes over time for control (CK) and 5mg NS/L pulse treatments
in numbers of vase solution and stem-end bacteria for cut gerbera cv.
Ruikou flowers (n = 3).
29
Case study 2
30
31
Fig. 4 Effects of chitosan or/and nano-silica treatments on fruit browning index (a) in
loquat fruit
32
Fig. 5 Effects of chitosan or/and nano-silica treatments on fruit weight loss in
loquat fruit
33
Fig.6 Effects of chitosan or/and nano-silica treatments on contents of TSS in
loquat fruit
34
Fig. 7 Effects of chitosan or/and nano-silica treatments on contents of TA in
loquat fruit
35
Fig. 8 Effects of chitosan or/and nano-silica treatments on activities of PAL in
loquat fruit
36
Figure
Fig. 9 Effects of chitosan or/and nano-silica treatments on activities of
PPO enzyme in loquat fruit
37
Case study 3
38
Table 2 Composition, mean droplet diameter (Z-average) and
polydispersity index (PDI) of different nanoemulsions produced
for the encapsulation of essential
39
Fig. 12 Total microbial load of rucola samples
40
Fig. 13 Total microbial load of rucola samples treated with a bioactive
coating, consisting of nanoemulsified lemon EO with modified chitosan, in
comparison with the untreated samples(control) as function of the storage
time.
Time (Days)
41
Table 3 Evolution of global colour difference
ΔE*ab and of peak force in compression (N)
over time of rucola
samples
42
Case study 4
43
Fig. 15 Total soluble solids (TSS) of mangoes during 45 d of storage. Each
data point is the average of three determinations and the error bars show
the standard deviation.
17.63 ± 0.4 Brix
11.5 ± 0.3 B
15.8 ± 0.3 Brix
11.5 ± 0.05 B
13.3 ± 0.1 Brix
44
Fig 16 Mass loss of mangoes during 45 d of storage. Each data point is the
average of three determinations and the error bars show the standard
deviation
3.26 ± 0.25%
1.8±0.16%
45
Fig. 17 Titratable acidity (TA) of mangoes during 45 d of storage. Each data
point is the average of three determinations and the error bars show the standard
deviation.
0.77 ± 0.3 (%)
0.80 ± 0.01 (%)
0.12 ± 0.01 (%)
0.40 ± 0.01 (%),
Health issues
Nanoparticles could be inhaled, swallowed, absorbed
through skin
they trigger inflammation and weaken the immune system.
And interfere with regulatory mechanisms of enzymes
and proteins
Environmental issues
Nanoparticles could accumulate in soil, water and plants
New risk assessment methods are needed
National and international agencies are beginning to
study the risk
 Results will lead to new regulations
46
Potential Risks of Nanotechnology
47
The efficiency and the economic benefits of applying various
techniques in combination with nanotechnology needs to be
evaluated in the different Horticultural produce.
Focused research is required in use of nanoparticles to improve
the quality and the post harvest life of Horticultural produce.
Future prospects
48
49
Nano

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Nano-coating ,Nano-treatments novel approaches to Extend the post harvest life of Horticultural produce

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  • 6. Nano-coating , Nano-treatments novel approaches to Extend the post harvest life of Horticultural produce Aisha Kolhar UHS15PGM620 Dept of Post Harvest Tech Seminar I on
  • 7. 7 Properties of Nano Particles Case Studies Conclusion Mechanism of Nano Particles History of nanotechnology Introduction to nanotechnology
  • 8. Nanotechnology ?... “Nanotechnology is the art and science of manipulating matter at nanoscale” The design, characterization, production and application of structure, device and system by controlling shape and size at nanoscale British standard institution, 2005 8
  • 9. ‘Nano’- Greek word means ‘Dwarf’  1 nm = one billionth (10ˉ⁹) of metre  Size range between 0.1 and 100 nm 9
  • 10. 10
  • 11. “There’s Plenty of Room at the Bottom” - at American Physical Society meeting at the California Institute of Technology on Dec-29, 1959. “Adaptability to manipulate, control, assemble, produce and manufacture things at atomic precision” 11 Father of nano technology Nobel Laureate-1965 Richard Feynman, Physicist
  • 12. Norio Taniguchi, Professor - coined the term “Nanotechnology” (1974) “Nano-technology’’ - Processing, separation, consolidation and deformation of materials by one atom or by one molecule. 12
  • 13. ~ 2000 Years Ago Sulfide nanocrystals used by Greeks and Romans to dye hairs ~ 1000 Years Ago Gold nanoparticles of different sizes used to produce different colors in stained glass windows 1959 “There is plenty of room at the bottom” by R. Feynman 1974 Taniguchi uses the term nanotechnology for the first time 1981 IBM develops Scanning Tunneling Microscope 1985 “Buckyball” - Scientists at Rice University and University of Sussex discover C60 1986 • “Engines of Creation” - First book on nanotechnology by K. Eric Drexler. • Atomic Force Microscope invented by Binnig, Quate and Gerbe 1989 IBM logo made with individual atoms 1991 Carbon nanotube discovered by S. Iijima 1999 “Nanomedicine” – 1st nanomedicine book by R. Freitas 2000 “National Nanotechnology Initiative” launched (British Standards Institution, 2005) Time Line of Nanotechnology 13
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  • 15. Properties of nano particles 15  Nano-scale materials show unusual physical, chemical and biological properties. (Li et al., 2001)  Nanoparticles have large surface to volume ratio (Kumar et al., 2010) Magical changes takes place at Nano level Physical Surface area, conductivity, charge capacity Chemical Reactivity Mechanical strength
  • 16. Things behave differently in nano-world  Carbon in the form of graphite (i.e. pencil lead) is soft, at the nano-scale, can be stronger than steel and is six times lighter  Nano-scale copper is a highly elastic metal at room temperature, stretching to 50 times its original length without breaking  Shiny orange yellow Gold changes its colour to brownish black on reducing the size 16
  • 17. (Royal Society and Royal Academy of Engineering, 2004) METHODS OF NANOPARTICLE PRODUCTION 17
  • 18. (Royal Society and Royal Academy of Engineering, 2004) TOP DOWN APPROACH 18
  • 19. (Royal Society and Royal Academy of Engineering, 2004) 19 BOTTOM UPAPPORAOCH
  • 21. 21 Why we need Nanotechnology ??? To enhance the efficiency of available post harvest technologies To reduce the economics of additives. Ethylene biosynthesis inhibitors – AVG,AOA : Expensive and Phytotoxic Many chemicals which we are using may be soon banned due to their environmental impact
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  • 26. 26 Fig.1 Changes in vase water uptake over time of cut gerbera cv. Ruikou flowers following NS pulse treatments
  • 27. 27 Fig.2 Changes in relative fresh weight (B) over time of cut gerbera cv. Ruikou flowers following NS pulse treatments
  • 28. 28 Fig. 3 Changes over time for control (CK) and 5mg NS/L pulse treatments in numbers of vase solution and stem-end bacteria for cut gerbera cv. Ruikou flowers (n = 3).
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  • 31. 31 Fig. 4 Effects of chitosan or/and nano-silica treatments on fruit browning index (a) in loquat fruit
  • 32. 32 Fig. 5 Effects of chitosan or/and nano-silica treatments on fruit weight loss in loquat fruit
  • 33. 33 Fig.6 Effects of chitosan or/and nano-silica treatments on contents of TSS in loquat fruit
  • 34. 34 Fig. 7 Effects of chitosan or/and nano-silica treatments on contents of TA in loquat fruit
  • 35. 35 Fig. 8 Effects of chitosan or/and nano-silica treatments on activities of PAL in loquat fruit
  • 36. 36 Figure Fig. 9 Effects of chitosan or/and nano-silica treatments on activities of PPO enzyme in loquat fruit
  • 38. 38 Table 2 Composition, mean droplet diameter (Z-average) and polydispersity index (PDI) of different nanoemulsions produced for the encapsulation of essential
  • 39. 39 Fig. 12 Total microbial load of rucola samples
  • 40. 40 Fig. 13 Total microbial load of rucola samples treated with a bioactive coating, consisting of nanoemulsified lemon EO with modified chitosan, in comparison with the untreated samples(control) as function of the storage time. Time (Days)
  • 41. 41 Table 3 Evolution of global colour difference ΔE*ab and of peak force in compression (N) over time of rucola samples
  • 43. 43 Fig. 15 Total soluble solids (TSS) of mangoes during 45 d of storage. Each data point is the average of three determinations and the error bars show the standard deviation. 17.63 ± 0.4 Brix 11.5 ± 0.3 B 15.8 ± 0.3 Brix 11.5 ± 0.05 B 13.3 ± 0.1 Brix
  • 44. 44 Fig 16 Mass loss of mangoes during 45 d of storage. Each data point is the average of three determinations and the error bars show the standard deviation 3.26 ± 0.25% 1.8±0.16%
  • 45. 45 Fig. 17 Titratable acidity (TA) of mangoes during 45 d of storage. Each data point is the average of three determinations and the error bars show the standard deviation. 0.77 ± 0.3 (%) 0.80 ± 0.01 (%) 0.12 ± 0.01 (%) 0.40 ± 0.01 (%),
  • 46. Health issues Nanoparticles could be inhaled, swallowed, absorbed through skin they trigger inflammation and weaken the immune system. And interfere with regulatory mechanisms of enzymes and proteins Environmental issues Nanoparticles could accumulate in soil, water and plants New risk assessment methods are needed National and international agencies are beginning to study the risk  Results will lead to new regulations 46 Potential Risks of Nanotechnology
  • 47. 47 The efficiency and the economic benefits of applying various techniques in combination with nanotechnology needs to be evaluated in the different Horticultural produce. Focused research is required in use of nanoparticles to improve the quality and the post harvest life of Horticultural produce. Future prospects
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Editor's Notes

  1. Nanoscience and technology are today at the same stage as information technology was in 1960s and biotechnology was in the1980s.