Slides that would help a Science background students who are interested in animal studies. This slides are all about blood collection methods from laboratory animals with images and videos. Images and videos would help students for better understanding.
Recombinant DNA technology (Immunological screening)
BLOOD SAMPLE COLLECTION
1. Welcome to Journal
Club Presentation
Presented by,
Dnyanshree V. Tijare
Guide: Dr. Manoj Aswar
M. Pharm second year (Dept. of Pharmacology)
Rajarshi Shahu College of Pharmacy, Buldhana
3. Continue…
Blood sample collection in small
laboratory animals
Author : Parasuraman S. Ravindran R. Kesavan R
DOI no. : 10.4103/0976-500X.72350
Journal name : Journal of Pharmacology & Pharmacotherapeutics
Publication year : July-December 2010
4. Content
General principles of blood collection method
Common anaesthetic agents required for lab animals
General methods for blood collection
Discussion & References
5. General principles of blood
collection method
1. Protocol approved by the Institute animal ethics committee.
2. It should be least painful and stressful, Blood sample may be collected
under anesthesia or without anesthesia.
3. Adequate training (any species)
4. In general, blood sample is withdrawn from venous, arterial blood vessels or
heart chambers.
5. Frequency (Once in two weeks is ideal for nonrodents).
6. The estimated blood volume in adult animals is 55 to 70 ml/kg body weight.
24. Procedure
This procedure is recommended for terminal stage of study
Give V/ Y shape cut in rat abdomen, and remove intestine gently
Anesthetized the animal
The liver is pushed forward, and identified the inferior/ posterior vena cava
Insert 21 to 25G needle and collect the blood
25. Fluid Replacement
Lactated Ringer’s Solution (LRS) is the recommended
balanced crystalloid solution for fluid replacement.
Alternatively, 0.9% sterile isotonic saline may be used. For
adult mice, 1.0 ml of warmed LRS or isotonic saline can be
given by IP or SC administration. For adult rats, administer
5 -10 ml warmed LRS or 0.9% saline (½ of the total volume
via IP and ½ via SC routes).
26. Estimated total blood volume and
safe bleeding volume of selected
species (once every two week)
No. Animal Total blood volume
(ml/kg)
Max. bleeding (ml/kg)
(10% of BW)
1. Mice 79 7.9
2. Rat 64 6.4
3. Guinea pig 75 7.5
4. Mini pig 65 6.5
5. Hamster 78 7.8
6. Frog 95 9.5
7. Rabbit 56 5.6
8. Chicken 60 6.0
9. Cat 55 5.5
27. Recommended site for blood
collection
No. Animals Recommended sites and conditions
1. Mice "submandibular" or "facial", saphenous vein,
retro-orbital (anesthetized), cardiac
(anesthetized, terminal)
2. Rat Tail vein, saphenous vein, "submandibular" or
"facial", cardiac (anesthetized, terminal),
sublingual, jugular vein
3. Guinea pig, Hamster Saphenous, cardiac (anesthetized, terminal)
4. Rabbit Marginal ear vein, cardiac (anesthetized,
terminal)
5. Chicken Brachial/wing vein, jugular
28. References
• Hoff J, Rlagt LV. Methods of blood collection in the mouse. Lab
animals 2000;29:47-53.
• Blood sampling online. 2009; [12 screens]. Available
from:http://www.nc3rs.org.uk/bloodsamplingmicrosite/page.asp?id=3
13[Last cited on 2010 Fe24].
• Guidelines for Survival Bleeding of Mice and Rats. NIH-ARAC
Guidelines [online]. 2005 Jan 12. Available from
http://oacu.od.nih.gov/ARAC/Bleeding.pdf [accessed on 2010 Mar
09].
• McGuill MW, Rowan AN. Biological Effects of Blood Loss:
Implications for Sampling Volumes and Techniques. ILAR J 1989. p.
31
29. Continue…
• Procedure for rabbit blood collection [online]. Available from:http://
www.research.uky.edu/ori/univet/resources/sop/Procedure_rabbit_bl
ood_ collection.pdf [Last cited on 2010 Feb 23].
• CPCSEA guidelines for laboratory animal facility. Indian J
Pharmacol 2003;35:257-74.
• Vogel HG, editor. Drug discovery and evaluation: Pharmacological
assays. 2nd ed. Berlin: Springer; 2002.
• Anaesthesia and Analgesia in Laboratory Animals at UCSF [online].
Available from :http://www.iacuc.ucsf.edu/Index.asp[Last cited on
2010 Feb 24]. and Availabl
from:http://www.iacuc.ucsf.edu/Proc/awRatFrm.asp. [Last cited on
2010 Feb 24].