8. PACKED CELL VOLUME
(or)HAEMATOCRIT
DEFINED AS THE VOLUME OF PACKED RED CELLS IN A GIVEN
SAMPLE OF BLOOD
EXPRESSED AS A PERCENTAGE OF THE TOTAL VOLUME OF THE
BLOOD SAMPLE
PACKING THE RED CELLS IS ACHIEVED BY CENTRIFUGING A
COLUMN OF BLOOD IN A GLASS TUBE
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/2017
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9. PACKED CELL VOLUME
(or)HAEMATOCRIT
IN ORDER TO STANDARDIZE THE DEGREE OF PACKING DURING
CENTRIFUGATION,
IT IS NECESSARY TO HAVE
(1) OPTIMUM SPEED OF THE CENTRIFUGE
(11) OPTIMUM RADIUS OF THE CENTRIFUGE
(111)OPTIMUM DURATION OF CENTRIFUGING
3/OCTOBER
/2017
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10. PACKED CELL VOLUME
(or)HAEMATOCRIT
DETERMINATION OF PCV
SIMPLE
VERY ACCURATE
METHOD OF ESTIMATING THE RED BLOOD CELL MASS IN THE
BLOOD OF TELLING WHETHER THE PATIENT IS NORMAL
ANAEMIC OR INCREASED RBC MASS(POLYCYTHEMIA)
3/OCTOBER
/2017
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17. PACKED CELL VOLUME
(or)HAEMATOCRIT
METHODS OF ESTIMATION OF PCV
1.MACRO METHOD USING WINTROBE’S METHOD
2.MICROMETHOD USING CAPILLARY TUBE
3.AUTOMATED ANALYZER
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/2017
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18. PACKED CELL VOLUME
(or)HAEMATOCRIT
ANTICOAGULANTS USED :EDTA SAMPLE ,HEPARIN OR
DOUBLE OXALATE (THE SPECIMEN NEED NOT BE A FASTING
SAMPLE)
PRINCIPLE: WHEN ANTICOAGULATED BLOOD IS CENTRIFUGED
IN A HAEMATOCRIT TUBE AT HIGH SPEED,THE ERYTHROCYTES
SEDIMENT AT THE BOTTOM.THE RED CELL COLUMN IS CALLED
AS PACKED CELL VOLUME(OR) HEMATOCRIT (CELL VOLUME%)
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19. REQUIREMENT
SPECIMEN
WINTROBE TUBE
PASTEUR PIPETTE (OR ) A SYRINGE WITH NEEDLE
CENTRIFUGE
WINTROBE TUBE
SPECIALLY THICK WALLED GLASS TUBE
GLASS TUBE 11 cm LONG
INTERNAL DIAMETER 3 mm
FLAT INNER BASE
CALIBRATED
HOLDS 1ml OF BLOOD
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20. PACKED CELL VOLUME
(or)HAEMATOCRIT
PROCEDURE
MIX THE BLOOD SAMPLE CAREFULLY
LABEL A WINTROBE TUBE
FILL THE TUBE USING PASTEUR PIPETTE OR A SYRINGE UP TO
THE 100 MARK (USE A NEEDLE OF WIDE BORE TO PREVENT
MECHANICAL BREAK DOWN OF RED BLOOD CELLS)
AVOID TRAPPING OF AIR BUBBLES
PLACE THE TUBE IN A CENTRIFUGE CUP(USE ANOTHER EMPTY
WINTROBE TUBE FILLED WITH ANOTHER SPECIMEN)
CENTRIFUGE FOR 30 MINUTES AT 3000 RPM
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21. • OBSERVATION
AFTER THE BLOOD IS CENTRIFUGED ,THREE DEFINITE LAYERS
CAN BE EASILY SEEN
UPPER MOST LAYERS – IS PLASMA,PALE YELLOW IN COLOUR
A THIN LAYER OF WBC AND PALTELETS WHICH IS JUST ABOVE
THE RBC,WHICH IS GREYISH IN COLOUR CALLED THE ‘BUFFY
COAT’(BUFFY LAYER -NORMALLY IT IS 0.5 TO 1mm.EACH 0.1ml =
1000 CELLS per cu mm (µl) approximately)
LOWER MOST LAYER OF RED CELLS ARE CLOSELY PACKED
TOGETHER
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/2017
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22. COLOR AND OPACITY OF PLASMA EXPECTED CLINICAL CONDITIONS
YELLOW JAUNDICE
MILKY LIPEMIA
CLOUDY MULTIPLE MYELOMA
REDDISH HEMOLYSIS
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23. OTHER USE OF WINTROBE
TUBE
• USED FOR ESR ESTIMATION
• THE WESTERGREN METHOD IS PREFERRED AS IT IS MORE
ACCURATE
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31. MICROHAEMATOCRIT METHOD
SPECIMENS
• EDTA OR OXALATED SPECIMEN(USE PLAIN CAPILLARY TUBES)
• CAPILLARY BLOOD(USE HEPARINIZED BLOOD)
PRINCIPLE
• BLOOD IS CENTRIFUGED IN A SEALED CAPILLARY TUBE AND PCV IS
DETERMINED BY, A SPECIAL HEMATOCRIT READER
REQUIREMENTS
• HEMATOCRIT CENTRIFUGE
• HEMATOCRIT READER
• CAPILLARY HEMATOCRIT TUBES
• SOFT WAX OR MODELLING CLAY
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32. PROCEDURE
• DRAW THE SPECIMEN INTO AN APPROPRITATE CAPILLARY
TUBE
• FILL IN THE TUBE TO ABOUT 2/3 LENGTH
• SEAL BOTH THE ENDS OF THE TUBE WITH SOFT WAX OR
MODELLING CLAY
• IT IS PLUGGED TO A DEPTH OF ABOUT 1 CENTIMETER
• PLACE THE TUBE WITH ANOTHER SIMILAR TUBE IN THE
RADIAL GROOVES OF THE CENTRIFUGE HEAD EXACTLY
OPPOSITE TO EACH OTHER
• CLOSE THE CENTRIFUGE COVER AND CENTRIFUGE THE TUBES
AT HIGH SPEED 15,000 RPM FOR 5 MINUTES
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33. • REMOVE THE CAPILLARY TUBE
OBSERVATION
• IT WILL SHOW THREE LAYERS
• 1.CLEAR PLASMA AT THE TOP
• 2.WHITISH- BUFFYCOAT AT THE MIDDLE
• 3.COLUMN OF RED AT THE BOTTOM
READ PCV AS FOLLOWS
• HOLD THE TUBE AGAINST THE HEMATOCRIT SCALE SO THAT THE BOTTOM
OF THE COLUMN OF RED CELLS IS ALIGNED WITH HORIZONTAL ZERO LINE
THE HEIGHT OF CLAY
• MOVE THE TUBE ACROSS THE SCALE UNTIL THE LINE MARKED 1.0 PASSES
THROUGH THE TOP OF THE PLASMA COLUMN
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34. • THE LINE THAT PASSES THROUGH THE TOP OR THE COLUMN
OF RED CELLS GIVES THE VALUE OF PCV
SOURCE OF ERROR
• HEMOYSZED SPECIME WILL YIELD FALSE LOW VALUES
• INADEQUATE MIXING OF BLOOD
• INCOMPETENESS OF PACKING MAY LEAD TO ERRONEOUS
RESULTS
• ERROR UNDER OPTIMUM CONDITIONS IS ONLY ± 1%
DISADVATAGES
• IT REQUIRES SPECIAL CENTRIFUGE
• DISPOSABLE CAPILLARY TUBES
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35. ADVANTAGES
AMOUNT OF BLOOD REQUIRED IS VERY SMALL
EASILY BE OBTAINED FROM A FINGER PRICK
EDTA blood
TIME REQUIRED FOR CENTIFUGATION IS MUCH LESS
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36. CLINICAL CONDITIONS
FALL IN HEMATOCRIT
• ANEMIA
• HYDREMIA
• HEMORRHAGE
• CIRRHOSIS
• RENAL DISEASES
• NORMAL PREGNANCY
3/OCTOBER
/2017
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38. PREVIOUS YEAR QUESTION
PAPER
PCV ( 2MARKS) APRIL 2017
PACKED CELL VOLUME (AUGUST 2010/SEPTEMBER 2014 ) 5
MARKS
WINTROBE,S TUBE (FEB/MARCH 2011) 3 MARKS
BUFFY COAT (AUGUST 2010) 3 MARKS
WHAT IS PCV? NAME THE INSTRUMENT ,ANTICOAGULANT
AND NORMAL VALUES? (SEP/OCT 2008)
PCV TUBE(WINTROB’S TUBE) 3 MARKS (AUGUST 2013)
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39. PACKED CELL VOLUME
NORMAL RANGE
MEN : 42-52 %
WOMEN :36-48%
LATE PREGNANCY : 23-37%
3/OCTOBER
/2017
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