This document discusses several methods for developing monoclonal antibodies: hybridoma technology which fuses B cells and myeloma cells to produce hybridomas; surface display technology which uses genetic engineering to display antibody fragments on surfaces like phage, bacteria or yeast for screening; and transgenic technology which uses transgenic mice to produce monoclonal antibodies. It also describes Creative Biolabs' services for monoclonal antibody development including hybridoma generation and screening as well as in vitro surface display technologies.

1. Development of Monoclonal Antibodies
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com

2. Development of Monoclonal Antibodies
Monoclonal
Antibodies
Hybridoma
Technology
Surface
Display
Direct
Screening
B-cell
Immortalization
Transgenic
Technology

3. Hybridoma Technology
Cell culture Myeloma cells
Immunize mouse B cells
Hybridomas
Screening

4. Surface Display Technology
B cells
VL+VH genes
Surface display library Screening

5. Transgenic Technology
Target IgG genes
Myeloma cells
Ag immunization
B cells
Hybridomas
Screening

6. B-cell Immortalization
EBV
CpG 2006
Memory B cells Immortalized B cells
Hybridomas

7. Direct Screening
Sera
Isolated B cells
from positive
individual
Screening Screening

8. Creative Biolabs’ One-stop Hybridoma Services
Antigen Preparation Immunization
Hybridoma Generation
and Screening
Antibody Production
and Purification

9. Creative Biolabs’ In Vitro Surface Display Services
Phage Display Bacteria Display Ribosome Display
Yeast Display
Mammalian Cell
Display

10. Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com
Development of Monoclonal Antibodies