What is antibody humanization? How to humanize the antibody? The definition and humanization process is illustrated by Creative Biolabs in this slide. If you have any questions, welcome to contact us at info@creative-biolabs.com. We will be more than happy to serve you!

1. Antibody Humanization Services
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com

2. Human Anti-Mouse Antibody (HAMA) Response
Mouse Hybridoma Mouse mAbs Human
What is antibody humanization?

3. How to humanize the antibody?
Grifting Methods for Antibody Humanization

4. Construction
of Fv 3D
Model
Identification of
Backmutations
Design of
Humanized
Sequences
Construction
of Humanized
Sequences
Test of
Humanized
Sequences
Choice of
Acceptor
Sequences
Analysis of
Donor
Sequences
Murine mAb
Human AbHumanized mAb
Design Cycle of Grafting-Based Methods for Antibody Humanization
How to humanize the antibody?

5. VL gene
Chimeric L gene
CL gene
VH gene
CH gene
Chimeric H gene
Light chain
expression vector
Heavy chain
expression vector
Complete Ab
expression vector
Expression
in E. coli
Murine mAb
Human Ab
Partially
humanized mAb
Workflow of Grafting-Based Methods for Antibody Humanization
How to humanize the antibody?

6. Light chain of
murine mAb
Phage display library of
human Ab heavy chain
Human heavy chain
with highest-affinity
to the antigen
Phage display library of
human Ab light chain
Workflow of Chain-Shuffling Method for Antibody Humanization
Fully humanized
mAb
How to humanize the antibody?

7. Antibody Humanization Services Provided by Creative Biolabs
Antibody Humanization
Non-human Primate (NHP)
Antibody Humanization
Bovine Ultralong CDR3 Antibody
Humanization
Humanized Antibody
Germinalization
Magic™ Human Antibody
Production Using Transgenic Mice
De Novo Human/Humanized
Antibody Sequencing Service

8. Creative Biolabs’ De Novo Antibody Sequencing Services
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com
Antibody Humanization Services Provided by Creative Biolabs