bryopreservation

1. CRYOPRESERVATION

2. Cryopreservation is a process where cells,
whole tissues, or any other substances
susceptible to damage caused by chemical
reactivity or time are preserved by cooling to
sub-zero temperatures usually below -150°C..

3.  At low enough temperatures, any enzymatic or
chemical activity which might cause damage to the
material in question is effectively stopped.
 Cryopreservation methods seek to reach low
temperatures without causing additional damage
caused by the formation of ice during freezing.
 Traditional cryopreservation has relied on coating the
material to be frozen with a class of molecules termed
cryoprotectants

4. • Semen in semen cryopreservation
• Blood
Special cells for transfusion Stem cells. It is optimal in
high concentration of synthetic serum, stepwise
equilibration and slow cooling

5.  Umbilical cord blood Further information: Cord
 Cryopreservation
Tissue samples like tumors and histological cross sections
 Eggs (oocytes) in oocyte cryopreservation
 Embryos at cleavage stage (that are 2, 4 or 8 cells) or at blastocyst stage,
in embryo cryopreservation
 Ovarian tissue in ovarian tissue cryopreservation
 Plant seeds or shoots may be cryopreserved for conservation purposes
 blood

6. Natural cryopreservation
Water-bears (Tardigrada), microscopic
multicellular organisms, can survive freezing
by replacing most of their internal water
with the sugar trehalose, preventing it from
crystallization that otherwise damages cell
membranes.

7. Is an example for natural cryopreservation in which
frog can servive during heavy winter upto 65% body
water get freeze

8. ARTIFICIAL CRYOPRESERVATION
Here the living matter can preserved
in a artificial manner under
subzero temp.
It requires suffisticated labs

9. The branches of physics and engineering that involve
the study of very low temperatures, how to produce
them, and how materials behave at those
temperatures.

10. Lq Helium 222K
Lq Hydrogen 397K
Lq Neon 102K
Lq Nitrogen 348K
Lq Carbon di oxide 210k
Lq argon 260K

11. Helium -
Neon -245°C
nitrogen -
Carbon dioxide -20°C
argon

12. Nitrogen -210•c
Carbon di oxide -78.5•C
Helium- impossible to solidify
Neon -149°𝐶