This topic revels about IVF term embryo sexing for bsc biotechnology students

1. Made by :
Vipin Kr. Shukla
HIMT College Gr. Noida

2.  Pre Selection of Sex.
 Earlier approaches superstition based.
 Modern techniques make it possible.
 Either X-Y Sperm spotting or embryo sexing.

3.  Sex determined in Pre-Implantation embryos.
 Approach either Invasive or Non-Invasive.
 Splitted of seed embryos.
 First succesfully embryos sexing done by
Gardner (1968) in Rabbits by cytological
method (Barr-Body observation).

4.  Non-Invasive Methods:
Immunological assay of H-Y Antigen.
Quantification of X-Linked enzyme.
Differential growth of male and female embryo.

6.  Invasive methods:
 Cytogenetics analysis:
 Observing Barr Bodies
 Chromosome analysis
 Y- specific DNA probes
 Y-specific DNA Primer and PCR

7.  H-Y Detection used for Murine, Bovine,
Porcine and embryo.
 Detection as early as 8 cell stage
 Can have two approaches Cytotoxic or
Immuno-Fluorscent.
 In pigs detected only after removal of zona
pellucida.
 Accuracy 84%, Cattle 85%, 81% Pigs.

12.  Altering the male and female sex ratio in the
farm animals.
 Increase in milk and meat production.
 Control of incidence of free martinism.
 Getting equality bulls for sexing.
 Conversion of rare breeds of farm animals.