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SEMINAR
ON
GP 510: Breeding for Biotic and Abiotic Stress
Resistance
in
Department of Genetics and Plant Breeding
Date: 8th February 2017
Significant deviation from optimal conditions for
life that adversely affect growth, development
and productivity.
 Abiotic stress is the major limitation to crop production
worldwide.
 Depending on crop Biotic and Abiotic stresses reduce
average productivity by 65% to 87%.
 For instance, Bray et al. (2000) estimates that 51–82% of
the potential yield of annual crops is lost due to abiotic
stress.
 Stress act as catalyst in producing free
radical reactions where ROS (reactive
oxygen species) produced.
 ROS - O2
- superoxide radicals;
OH hydroxyl radical;
RO alkoxy radicals and
H2O2 hydrogen peroxide
Stress
Plant
cell
ROS
OXIDATIVE
STRESS
DEAT
H
Abiotic Biotic
Abiotic
Stress
Factors
Temperature
LightMineral
1. High temperatures
2. Low temperatures
1. High light intensity
2. Low light intensity
1.Acidity
2.Salinity
3.Alkalinity
1. Flood
2. Drought
Toxicity
Deficiency
Character of Abiotic stress
Location specific.
Unpredictable
Additive nature
Loss depends on stage of occurrence
and species.
High light: excess excitation of
photosynthetic centres,
accumulation of various ROS.
High temperature:
denaturation of protein and
lipid peroxidation.
Water deficit: draught stress,
ROS production,
Salinity: ion toxicity, mineral
stress, osmotic stress.
Cold stress: alters
membrane activity, metabolic
activity, injure cells and
tissues.
High temperature (>350C)
 Denature proteins and also
heavy water loses.
Low temperature (12 to 130C,
while -0 to -50C is lethal)
 Tropical plants more sensitive
 Formation of ice crystals.
Disruption of cell membranes
 Intercellular ice formation will
lead to drought situation
Reasons-Low percolation rate, Heavy soil, Heavy
downpour.
 Decreased O2 availability in root zone.
 Lowered ATP production
Major abiotic stress, Induced by
many environmental
conditions:
 No rainfall
 High salt concentration (reverse
osmosis)
 Low/High temperature
 Transient loss of turgor/soil
porosity/structure and texture
 Flow of water is
reversed- Reverse
osmosis
 Accumulation of
potentially toxic,
carbonate and
chloride ions.
Temperature
Nutrien
t
Moisture
Escape
Avoidance
Tolerance
Draught survival
Salt tolerance mechanism
Ion Exclussion
Ion excretion
Cell level
compartmentation
Plant level
compartmentation
Transpirational
cooling
Leaf rolling
Osmo
protectants
Early
maturation
Expression of
stress protein
Changing leaf
orientation
High
temperature
stress
survivability
Crop Variety Salient feature
Tomato
Pusa Sheetal Fruit setting at 80C night temperature
Pusa Sadabahar
Thermo-insensitive (grown at 8-300C night
temperature), fruit set takes place almost
round the year, both hot and cold set
Arka Ashish, Tolerant to Cracking
Cucumber
Poinsette Gynoecious cucumber
Pusa Barkha
Tolerant to high humidity and temperatures
(kharif variety)
Sponge gourd Pusa Sneha Tolerant to high Temperature
Cabbage
Pusa Ageti Tropical cabbage (Forms head15-300C)
September Resistant to cracking
Cauliflower
Pusa Early Synthetic Riceyness resistant
Pusa Snowball Self blanching
Cowpea
Arka Garima Heat and Drought tolerant
Pusa Dofasli Photo insensitive
 In conclusion, to meet the global food
requirement under emerging stress
conditions,
› Stress physiology with respect to different
crops should be studied under specific
regions.
› Improved breeding methods incorporated for
the development of sustainable varieties.
Abiotic stress classification and factors

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Abiotic stress classification and factors

  • 1. SEMINAR ON GP 510: Breeding for Biotic and Abiotic Stress Resistance in Department of Genetics and Plant Breeding Date: 8th February 2017
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  • 3. Significant deviation from optimal conditions for life that adversely affect growth, development and productivity.
  • 4.  Abiotic stress is the major limitation to crop production worldwide.  Depending on crop Biotic and Abiotic stresses reduce average productivity by 65% to 87%.  For instance, Bray et al. (2000) estimates that 51–82% of the potential yield of annual crops is lost due to abiotic stress.
  • 5.  Stress act as catalyst in producing free radical reactions where ROS (reactive oxygen species) produced.  ROS - O2 - superoxide radicals; OH hydroxyl radical; RO alkoxy radicals and H2O2 hydrogen peroxide Stress Plant cell ROS OXIDATIVE STRESS DEAT H
  • 7. Abiotic Stress Factors Temperature LightMineral 1. High temperatures 2. Low temperatures 1. High light intensity 2. Low light intensity 1.Acidity 2.Salinity 3.Alkalinity 1. Flood 2. Drought Toxicity Deficiency
  • 8. Character of Abiotic stress Location specific. Unpredictable Additive nature Loss depends on stage of occurrence and species.
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  • 10. High light: excess excitation of photosynthetic centres, accumulation of various ROS. High temperature: denaturation of protein and lipid peroxidation. Water deficit: draught stress, ROS production, Salinity: ion toxicity, mineral stress, osmotic stress. Cold stress: alters membrane activity, metabolic activity, injure cells and tissues.
  • 11. High temperature (>350C)  Denature proteins and also heavy water loses. Low temperature (12 to 130C, while -0 to -50C is lethal)  Tropical plants more sensitive  Formation of ice crystals. Disruption of cell membranes  Intercellular ice formation will lead to drought situation
  • 12. Reasons-Low percolation rate, Heavy soil, Heavy downpour.  Decreased O2 availability in root zone.  Lowered ATP production
  • 13. Major abiotic stress, Induced by many environmental conditions:  No rainfall  High salt concentration (reverse osmosis)  Low/High temperature  Transient loss of turgor/soil porosity/structure and texture
  • 14.  Flow of water is reversed- Reverse osmosis  Accumulation of potentially toxic, carbonate and chloride ions.
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  • 21. Salt tolerance mechanism Ion Exclussion Ion excretion Cell level compartmentation Plant level compartmentation
  • 22. Transpirational cooling Leaf rolling Osmo protectants Early maturation Expression of stress protein Changing leaf orientation High temperature stress survivability
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  • 24. Crop Variety Salient feature Tomato Pusa Sheetal Fruit setting at 80C night temperature Pusa Sadabahar Thermo-insensitive (grown at 8-300C night temperature), fruit set takes place almost round the year, both hot and cold set Arka Ashish, Tolerant to Cracking Cucumber Poinsette Gynoecious cucumber Pusa Barkha Tolerant to high humidity and temperatures (kharif variety) Sponge gourd Pusa Sneha Tolerant to high Temperature Cabbage Pusa Ageti Tropical cabbage (Forms head15-300C) September Resistant to cracking Cauliflower Pusa Early Synthetic Riceyness resistant Pusa Snowball Self blanching Cowpea Arka Garima Heat and Drought tolerant Pusa Dofasli Photo insensitive
  • 25.  In conclusion, to meet the global food requirement under emerging stress conditions, › Stress physiology with respect to different crops should be studied under specific regions. › Improved breeding methods incorporated for the development of sustainable varieties.