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A POWERPOINT PRESENTATION ON
GENERAL FEATURES OF PROKARYOTES
PRESENTED BY
AQUIB RAZA
M.Sc. I SEMESTER
DEPARTMENT OF MICROBIOLOGY
KNIPSS (SULTANPUR)
INTRODUCTION
•All living organisms on earth are composed of one or the other
of two types of cells; prokaryotic cells and eukaryotic cells based
on differences in cellular organization and biochemistry.
•Prokaryotic cells (pro or primitive) do not have a membrane-
bound nucleus. Prokaryotes includes Bacteria and Archaea. All
bacteria and blue green algae are prokaryotes.
•Most prokaryotes are small, single-celled organisms that have a
relatively simple structure.
GENERAL FEATURES
• They lack nuclear membrane.
• Mitochondria, golgi bodies, chloroplast and
lysosomes are absent.
• The genetic material is present on a single
chromosome.
• The cell wall is made up of carbohydrates and
amino acids.
• They divide asexually by binary fission.
• The sexual mode of reproduction involves
conjugation.
PROKARYOTIC CELL
SHAPE
• Depending on their shape, bacteria are classified into several
varieties:
• Cocci: Cocci (s., coccus) are spherical, Eg., Streptococci etc.
• Bacilli: Bacilli (from baculus meaning rod) are related straight,
rod shaped. Eg., Mycobacterium tuberculosis etc.
• Spirilla: They are rigid, spiral shaped cells that usually tufts of
flagella at one or both ends. Example: Helicobacter pylori etc.
• Spirochetes: They are flexible, spiral-shaped bacteria that
have a unique, internal flagellar arrangement. Eg., Treponema
pallidium etc.
• Vibrios: They are curved or comma-shaped rods and derive
from their characteristic motility. Eg., Vibrio cholerae etc.
PROKARYOTIC
CELL ORGANIZATION
• Plasma membrane
• Cell wall
• Inclusion bodies
• Gas vacuole
• Ribosomes
• Nucleoid
• Plasmids
• Capsules and slime
layers
• Fimbriae and pili
• Flagella
PLASMA MEMBRANE
• Every prokaryote has a cell membrane, also known as
the plasma membrane. It is a double layer of lipid,
(Phospholipid) that separates the cell from the
outside environment.
• Functions:
• Retaining the cytoplasm.
• Control permeability.
• Transport electrons and protons for cellular
metabolism.
• Contain enzymes to synthesise and transport cell wall
substance.
FLUID MOSAIC MODEL OF PLASMA MEMBRANE
• The cell wall of prokaryotes is composed of
peptidoglycan.
• Functions :
• Maintain the cell’s shape.
• Protect the cell interior.
• Prevent the cell from bursting when cell takes
up water.
CELL WALL
GRAM POSITIVE AND GRAM NEGATIVE
CELL WALL
• Gram-positive bacteria lack an outer
membrane but are surrounded by layers of
peptidoglycan many times thicker than is
found in the Gram-negatives. Eg.,
Mycobacterium tuberculosis
• Gram-negative bacteria are surrounded by a
thin peptidoglycan cell wall, which itself is
surrounded by an outer membrane containing
lipopolysaccharide. Eg., E.coli
GRAM POSITIVE AND GRAM NEGATIVE CELL WALL
CAPSULE
• Many prokaryotes have a sticky outermost
layer called the capsule, which is usually
made of polysaccharides.
• The capsule helps pathogenic bacteria to
resist phagocytosis . Eg., Streptococcus
pneumoniae.
• They prevent the cell from drying out.
• They aid in attachment to solid surfaces.
PROKARYOTIC CYTOSKELETONS
INCLUSION BODIES AND GAS VACUOLE
1. Inclusion bodies are non-living granules of
organic and inorganic materials. These bodies
are used for storage.
2. Gas vacuole, a structure that provides
buoyancy to some aquatic prokaryotes.
• Gas vacuoles, are present in many
photosynthetic bacteria and a few other
aquatic prokaryotes such as halobacterium (a
salt loving archaeon).
INCLUSION BODY AND GAS VACUOLE
RIBOSOMES
• Ribosomes are very complex structures made of
both protein and ribonucleic acid (RNA). They are
the site of protein synthesis.
• Bacterial and archaeal ribosomes are called 70S
ribosome and are constructed of a 50S and a 30S
subunit.
PROKARYOTIC RIBOSOME
(30S SUBUUNIT AND 50S SUBUNIT)
NUCLEOID AND PLASMIDS
• The nucleoid is an irregularly shaped region that
contains the cell’s chromosome and numerous
proteins.
• Nucleoid is also known as nuclear region, the
nuclear body, and chromatin body.
• Plasmids are small, double-stranded DNA
molecules that can exist independently of the
chromosome. Both circular and linear plasmids
have been documented, but most known
plasmids are circular.
NUCLEOID AND PLASMID
EXTERNAL STRUCTURES
FIMBRIAE AND FLAGELLA
• Many bacteria and archaea have structures that
extend beyond the cell envelope. These structure can
function in protection, attachment to the surfaces, etc.
• FIMBRIAE- Many bacteria and archaea have short,
fine, harelike appendages known as Fimbriae.
• FLAGELLA- They are hairlike structure that acts
primarily as an organelle of locomotion in the cells of
many living organisms. Most motile prokaryotes move
by use of flagella.
FIMBRIAE AND FLAGELLA
𝓣𝓱𝓪𝓷𝓴 𝔂𝓸𝓾

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GENERAL FEATURES OF PROKARYOTES

  • 1. A POWERPOINT PRESENTATION ON GENERAL FEATURES OF PROKARYOTES PRESENTED BY AQUIB RAZA M.Sc. I SEMESTER DEPARTMENT OF MICROBIOLOGY KNIPSS (SULTANPUR)
  • 2. INTRODUCTION •All living organisms on earth are composed of one or the other of two types of cells; prokaryotic cells and eukaryotic cells based on differences in cellular organization and biochemistry. •Prokaryotic cells (pro or primitive) do not have a membrane- bound nucleus. Prokaryotes includes Bacteria and Archaea. All bacteria and blue green algae are prokaryotes. •Most prokaryotes are small, single-celled organisms that have a relatively simple structure.
  • 3. GENERAL FEATURES • They lack nuclear membrane. • Mitochondria, golgi bodies, chloroplast and lysosomes are absent. • The genetic material is present on a single chromosome. • The cell wall is made up of carbohydrates and amino acids. • They divide asexually by binary fission. • The sexual mode of reproduction involves conjugation.
  • 5. SHAPE • Depending on their shape, bacteria are classified into several varieties: • Cocci: Cocci (s., coccus) are spherical, Eg., Streptococci etc. • Bacilli: Bacilli (from baculus meaning rod) are related straight, rod shaped. Eg., Mycobacterium tuberculosis etc. • Spirilla: They are rigid, spiral shaped cells that usually tufts of flagella at one or both ends. Example: Helicobacter pylori etc. • Spirochetes: They are flexible, spiral-shaped bacteria that have a unique, internal flagellar arrangement. Eg., Treponema pallidium etc. • Vibrios: They are curved or comma-shaped rods and derive from their characteristic motility. Eg., Vibrio cholerae etc.
  • 6.
  • 7. PROKARYOTIC CELL ORGANIZATION • Plasma membrane • Cell wall • Inclusion bodies • Gas vacuole • Ribosomes • Nucleoid • Plasmids • Capsules and slime layers • Fimbriae and pili • Flagella
  • 8. PLASMA MEMBRANE • Every prokaryote has a cell membrane, also known as the plasma membrane. It is a double layer of lipid, (Phospholipid) that separates the cell from the outside environment. • Functions: • Retaining the cytoplasm. • Control permeability. • Transport electrons and protons for cellular metabolism. • Contain enzymes to synthesise and transport cell wall substance.
  • 9. FLUID MOSAIC MODEL OF PLASMA MEMBRANE
  • 10. • The cell wall of prokaryotes is composed of peptidoglycan. • Functions : • Maintain the cell’s shape. • Protect the cell interior. • Prevent the cell from bursting when cell takes up water. CELL WALL
  • 11. GRAM POSITIVE AND GRAM NEGATIVE CELL WALL • Gram-positive bacteria lack an outer membrane but are surrounded by layers of peptidoglycan many times thicker than is found in the Gram-negatives. Eg., Mycobacterium tuberculosis • Gram-negative bacteria are surrounded by a thin peptidoglycan cell wall, which itself is surrounded by an outer membrane containing lipopolysaccharide. Eg., E.coli
  • 12. GRAM POSITIVE AND GRAM NEGATIVE CELL WALL
  • 13. CAPSULE • Many prokaryotes have a sticky outermost layer called the capsule, which is usually made of polysaccharides. • The capsule helps pathogenic bacteria to resist phagocytosis . Eg., Streptococcus pneumoniae. • They prevent the cell from drying out. • They aid in attachment to solid surfaces.
  • 14. PROKARYOTIC CYTOSKELETONS INCLUSION BODIES AND GAS VACUOLE 1. Inclusion bodies are non-living granules of organic and inorganic materials. These bodies are used for storage. 2. Gas vacuole, a structure that provides buoyancy to some aquatic prokaryotes. • Gas vacuoles, are present in many photosynthetic bacteria and a few other aquatic prokaryotes such as halobacterium (a salt loving archaeon).
  • 15. INCLUSION BODY AND GAS VACUOLE
  • 16. RIBOSOMES • Ribosomes are very complex structures made of both protein and ribonucleic acid (RNA). They are the site of protein synthesis. • Bacterial and archaeal ribosomes are called 70S ribosome and are constructed of a 50S and a 30S subunit.
  • 18. NUCLEOID AND PLASMIDS • The nucleoid is an irregularly shaped region that contains the cell’s chromosome and numerous proteins. • Nucleoid is also known as nuclear region, the nuclear body, and chromatin body. • Plasmids are small, double-stranded DNA molecules that can exist independently of the chromosome. Both circular and linear plasmids have been documented, but most known plasmids are circular.
  • 20. EXTERNAL STRUCTURES FIMBRIAE AND FLAGELLA • Many bacteria and archaea have structures that extend beyond the cell envelope. These structure can function in protection, attachment to the surfaces, etc. • FIMBRIAE- Many bacteria and archaea have short, fine, harelike appendages known as Fimbriae. • FLAGELLA- They are hairlike structure that acts primarily as an organelle of locomotion in the cells of many living organisms. Most motile prokaryotes move by use of flagella.