Applications of chromatography

1. Applications of
Chromatography
Biological Techniques
BS ZOOLOGY 6th Semester
Presented to : Dr. Tamseela Mumtaz
Presentd by : Aitlas Sana Khan
Areeba Rafaqat

2. Applications of Chromatography in
pharmaceutical industries
• The technique of chromatography is extensively employed in the
pharmaceutical industry in order to analyze and identify the presence of
any trace amounts of chemicals and elements in a given sample.
• Another vital application of chromatography in this sector is in the
separation of certain chemical compositions based on their molecular
masses (and sometimes on the basis of the elements that constitute
them).
• The technique of chromatography also plays a crucial role in the
development of new drugs. For example, the presence of impurities and
other unknown compounds can be detected in the drug sample with the
help of chromatography. Furthermore, the purity of the drug sample can
also be analyzed with the help of this technique .

3. Applications of Chromatography in the Food
Industry
• In the food industry, the technique of chromatography plays a vital
role in the determination of the shelf life of food substances by
helping in the analysis of the point at which food spoils.
• Furthermore, the presence of chemical additives in food can also be
determined with the help of this technique.
• The nutritional value of the food sample can also be determined by
employing chromatographic techniques.
• Measurement of quality of soft drinks and water.
• Sugar analysis in fruit juices.

4. Applications of Chromatography in the
Chemical Industries
• Chromatography plays a vital role in the chemical industry for the
testing of water samples for purity.
• The testing of air samples for their purity is also accomplished by
chromatographic techniques in the chemical industry.
• The presence of toxic contaminants in oils and pesticides (the most
notable of which being polychlorinated biphenyls, often abbreviated
to PCBs) can be determined with the help of specialized
chromatographic techniques such as GC and HPLC.
• It can also be noted that chromatography also has many
applications in the life sciences.

5. Applications of Chromatography in the Field of
Molecular Biology
• In the field of molecular biology, the study of proteomics and
metabolomics often involve the use of various hyphenated
chromatographic techniques (the most notable of which being EC-
LC-MS).
• Nucleic acid research is also known to make extensive use of such
chromatographic techniques.
• A specific type of chromatography known as HPLC is widely used in
protein separation applications. This type of chromatography is also
useful in enzyme purification, plasma fractionation, and insulin
purification.

6. Applications in Clinical Tests
• Urine analysis, antibiotics analysis in blood.
• Analysis of bilirubin, biliverdin in hepatic disorders.
• Detection of endogenous neuropeptides in extracellular fluid of brain etc.
Environmental Applications
• Detection of phenolic compounds in drinking water.
• Bio-monitoring of pollutants.

7. Application in hospital
• Detect blood or alcohol level in a patient blood stream.
Law Enforcement
• To compare a sample found at a crime scene to samples from suspects.
Environmental Agency
• Determine the level of pollutants in the water supply

8. Applications…
• It is also used for the analysis of drugs, hormones and vitamins.
• Helpful for the qualitative and quantitative analysis of complex
mixtures .
• The technique is also used for determination of molecular weight of
proteins.
• It is used in forensic science for detecting the presence of drugs.
• It is used in pharmacy for detecting the chiral compounds
(Enantiomers and optical isomers).

9. THANK
YOU