The segments of plants that have medicinal value and mending properties are called as bioactive compounds and they experience a progression of the procedure called as Extraction, Isolation, and Characterization. Column Chromatography strategy for plant removes is one such method for confining and distinguishing the bioactive compounds utilizing a stationary stage, for example, Silica gel or Alumina. The Development of logical strategies needs to prompt a subjective and in addition quantitative examination of Isolation of bioactive compounds from Plants which are herbs with a muddled arrangement of blends framed through different pathways.

1. Isolation of
bioactive
compounds from
medicinal plants
using
chromatography
technique
column-chromatography.com

2. • Chromatography has been a fundamental technique used for chemical
separation. Specifically, column chromatography, typically taught in
introductory organic chemistry laboratories. This situation runs
contrary to the principles of responsible chemical and waste
management emphasized by the green chemistry movement.
• Chromatographic techniques have significant role in natural products
chemistry as well as contribute dramatically in the discovery of novel
and innovative compounds of pharmaceutical and biomedical
importance.
• This study focused on step-by-step visual demonstration of
fractionation and isolation of biologically active plant secondary
metabolites using column-chromatographic techniques.
column-chromatography.com

3. • Column chromatography is devised on the basis of differential
absorbance of substances on solid adsorbent i.e. silica gel or
alumina oxide to an extent that depends on the substance polarity
and other chemical properties and structural properties.
a. Silica Gel b. Alumina Oxide
column-chromatography.com

4. Isolation of bioactive
compounds using column-
chromatographic involves:
a) Preparation of sample;
b) Packing of column;
c) Pouring of sample into the
column;
d) Elution of fractions; and
e) Analysis of each fractions
using thin layer
chromatography.
However, depending on nature
of research, compounds can be
further purified using high
performance liquid
chromatography (HPLC), and
nuclear magnetic resonance
(NMR) spectral analyses.
column-chromatography.com

5. • Plants are healthy and natural resource of life. In particular,
medicinal plants are of great importance with endless therapeutic
properties useful for curing various diseases with an advantage of
being natural.
column-chromatography.com

6. • In past, the plant or microbial extracts in crude or partially-purified
forms were the only sources of medication available for the
treatment of human and animal diseases. This gave an idea that the
effect of a drug in human body is due to an interaction of drug with
biological molecules. This opened new doors in pharmacology, as
pure, isolated chemicals, instead of extracts, as the standard for the
treatment of diseases. At present, there are innumerable number of
such bioactive compounds isolated form crude extracts and their
chemical structure were elucidated.
column-chromatography.com

7. Moreover, plants have always
been a source of a wide array
of secondary metabolites with
potential pharmacological
properties. Polyphenol
(flavonoids) compounds occur
ubiquitously in foods of plant
origin have many beneficial
health effects due to their
potential anti-oxidant, anti-
inflammatory and cancer-
preventive activities.
column-chromatography.com

8. column-chromatography.com

9. • Therefore, the objective of this study was to provide step-by-
step visual demonstration of fractionation and isolation of
biologically active plant secondary metabolites using column-
chromatographic techniques. Isolation and purification of plant
secondary metabolites using column-chromatographic techniques.
column-chromatography.com

10. For More information
Contact Us:
SORBEAD INDIA
304-307, Sorbead India, Prayosha Complex,
Chhani Jakat Naka, Vadodara – 390024, Gujarat,
India
Visit: www.column-chromatography.com
Sales Inquiries: sales@sorbeadindia.com
Technical Inquiries: rajesh@sorbeadindia.com
column-chromatography.com