Polymerase Chain Reaction RAPD (Random Amplified Polymorphic DNA) DNA Amplification Fingerprinting Arbitrary-Primed PCR SCAR (Sequenced Characterised Amplified Region Marker) AFLP Procedure SSR (Simple Sequence Repeats) Inter-Simple Sequence Repeats Cleaved Amplified Polymorphic Sequences Single Strand Conformation Polymorphism Sequence Related Amplified Polymorphism Target Region Amplification Polymorphism Transposable Element-Based Markers Start codon targeted polymorphism CAAT Box Derived Polymorphism Conserved DNA-Derived Polymorphism  Intron-Targeting Polymorphism

1. PRESNTED BY
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•

2. 940C
2 min
400C - 600C
1 min
700C
2 min
Progressive addition of
nucleotides to the free 3’-OH
groups of the primers.

3. fi
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Leaf samples collected
from different plants

4. Isolation of
DNA
Keep the
tubes in PCR
Thermocycler
DNA strands
separated
Annealing of
primer (36°C)
05 06
Complemenary
strand synthesis
07 08
Band detected
by Ethidium
bromide staining
Primer annealed
to template DNA
strands
Amplified
products
separated by gel
electrophoresis
01 02 03 04
Denature
the
DNA
DNA
Synthesis
35
to
45
cycles

5. • fi fi fi
4–6 nt as primer.
• fi
• uses less stringent conditions for annealing and primer extension
•
• Short extension times fi
•

6. • 18–32 nt fi
• fi
mismatches
•
• fi

7. RAPD
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AP- PCR
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8. fi

9. • fi
•
•
fi
•
•

10. 
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fi fi fl

11.  fl
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12. 
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13. Advantages
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Disadvantages
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Applications
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Primer elements:
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