Shikimic acid pathway is "metabolic pathway. " ,means metabolites are organic compounds synthesized by plant using enzyme mediated chemical reactions called metabolic pathway . Now the metabolites are two types primary and secondary metabolites. The shikimic acid pathway is biosynthesis of secondary metabolites of phenylalanine, tyrosine and tryptophan(amino acid).

1. Shikimic Acid Pathway
Rina suresh patil
T.Y B pharmacy ,Roll No.Dsp 06
Principle: Dr.Amol .D. Landge sir.
Guided By : prof.M. K . Kachhava sir.

2. ■ what is the Shikimic acid. pathway?
■ Explain the Shikimic acid pathway and its
there significance?
Learning Outcomes

3. Shikimic acid pathway
• The shikimic acid pathway is a metabolic
pathway. for the bio synthesis of aromatic
amino acids.
Phenylalanine,tyrosine and tryptophan.
• The shikimic acid is a key intermediate from .
carbohydrates for the bio synthesis of C6 – C3
units (phenyl propane derivatives).

5. • The seven enzyme involved in the shikimate
pathway are DAHP synthesis, 3 –
dehydroquinate synthesis, 3- dehydroquinate
dehydratase, shikimate dehydrogenase ,
shikimate kinase , EPSP synthesis, and
chorismate synthesis.
• the pathway starts with two substrates ,
phosphoenol pyruvate and Erythrose -4-
phosphate and ends with chorismate.
• A substrate for the three aromatic amino acids.

6. Reflection Spot
What is basic metabolic pathway ? and
it’s their example ?

8. • Phosphoenolpyruvate and erythrose -4-phosphate react to
from 2-keto-3-deoxy-phosphoglucoheptonic acid, in a reaction
catalyzed by the enzyme DAHP synthesis.
Shikimic acid pathway
• 2-keto-deoxy-7-phosphoglucoheptonic acid is then transformed to
3-dehydroquinate (DHQ) is a reaction catalyzed by DHQ synthese.

9. • DHQ is dehydrated to 3-dehydroshikimic acid by the enzyme -
dehydroquinate dehydratase,which is reduced to shikimic acid by the
enzyme shikimate dehydrogenase , which uses nicotinamide adenine
dinucleotide phosphate (NADPH) as a contractor.
• the next enzyme involved is shikimic kinase, an enzyme that catalyzes
the ATP dependent phosphorylation of shikimate to form shikimate 3–
phosphate. Shikimate 3 -phosphate is then coupled with
phosphoenolpyruvate to give 5-enolpyruvylshikimate-3- phosphate via
the enzyme 5-enolpyruvylshikimate-3- phosphate synthese (EPSP).

10. • Then 5-enolpyruvylshikimate-3-phosphate is transformed
into chorismate by a chorismate synthase.
• Prephenic acid is then synthesized by a claisen
rearrangement of chorismate by chorismate mutate.

11. • Prephenate is Oxidatively decarboxylated with
retention of the hydroxyl group by prephenate
dehydrogenase to give p-hydroxyphenylpyruvate ,
which is transaminated using glutamate as the
nitrogen source to give tyrosine and alpha-
ketoglutarate.
• Now this prephenic acid give phenypyruvic acid on the
aromatization by enzyme prephenate dehydrogenase
which further on transamination give phenylalanine .
• Tyrosine and phenylalanine both are act as a
precursor for the bio synthesis of phenylpropanoids.
• Example: flavonoids and lignin. Etc.

12. • Shikimic acid is the starting point in the bio synthesis of many
phenolic compounds.
• Through shikimic acid pathway phenylalanine and tyrosine are
obtained which act as a precursor for the bio synthesis of
phenypropanoids .
• The phenylpropanoids are then used to produce flavonoids,
coumarins, lignin and tannins.
• Tryptophan produce from shikimic acid is a precursor of
Indole,Indole derivatives example for reserpine (Rauwolfia),
vincristine and vinblastin ( Vinka ) .
Significance

13. • Some many alkoinds are examples Quinine
(cinchona)and papaverine (Opium ).
• And other aromatic metabolites are examples for
vanilline and arbutine.
• As a intermediate in gallic acid synthesis pathways .

14. Got any questions ...
Questions

15. References
• Dr.M.G.kalaskar ,Dr.D.B.sanghai and S.B.Ghokhale , “A
textbook of pharmacognosy and phytochemistry –II ”,Nirali
prakashan ,fourth edition, January 2022,page no. 1.4 to 1.7.
• “ Plants Secondary Metabolism ” , by David. S.Seigler .page
no .94
• https://www.chips.ac.in/pages/downloads/PPTs/Pcognosy/shikimika
cidpathway.pdf

16. Thank You!!