Curcumin:Extraction of Curcumin Paper &Thin layer Chromatography, Antibacterial Activity of Curcumin
Plan of work
Introduction
Extraction of curcumin
Chromatography
Conclusion
2. COURSE STRUCTURE AND CONTENT FOR
PRACTICE SCHOOL (BP706PS)
B.PHARM FINAL YEAR (SEM VII)
DOMAIN-13
QUALITY ASPECTS OF HERBAL DRUG AND IT’S
FORMULATION
CURCUMIN: EXTRACTION OF CURCUMIN,
PAPER & THIN LAYER
CHROMATOGRAPHY, ANTIBACTERIAL
ACTIVITY OF CURCUMIN”
3. A REVIEW ARTICLE BY:
KURDHANE PARASRAM ASARAM
FINAL YEAR B.PHARM
(1952811823040)
GUIDED BY:PROF. UNMESH BHAMARE
5. CONTENT
1.Literature Survey
2.Aim &Objectives
3.Plan of work
4.Abstract
5.Introduction
6.Chemical constituents
7.Extraction of curcumin
8.Chromatography: Separation of Curcumin from curcuminoids using paper.
9.Paper Chromatography
10.Antibacterial Activity
11.Conclusion
12.Reference
6. LITERATURE SURVEY
• Prasad V Kadam, Kavita N Yadav, Chandrashekhar L Bhingare, Manohar J Patil.
Standardization and quantification of curcumin From Curcuma longa extract using UV
visible Spectroscopy and HPLC.
• Aleksandra Zielińska, Henrique Alves, and Eliana B. Souto.
Properties, Extraction Methods, and Delivery Systems for Curcumin as a Natural
Source of Beneficial Health Effects.
7. • Artur Adamczak, Marcin Ożarowski, and Tomasz M. Karpiński.
Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity.
Curcumin, a principal bioactive substance of turmeric (Curcuma longa L.), is
reported as a strong antioxidant, anti-inflammatory, antibacterial, antifungal,
and antiviral.
8. AIM & OBJECTIVES
• To extract curcumin from turmeric.
• To identify presence of curcumin by using Paper & Thin layer
chromatography.
• To check Antibacterial activity of curcumin by using microbiological
techniques.
9. PLAN OF WORK
• Making Overview of Curcumin.
• Collection of data regarding Curcumin extraction, it’s chemistry,
properties and its Uses.
• Extraction of curcumin from the dried root of the rhizome Curcuma
Longa l. using the soxhlet extraction method.
• Separation of curcumin with the help of chromatographic techniques
like Thin layer chromatography and paper chromatography and
calculation of Rf value.
10. ABSTRACT
• Curcumin is the main active component in Curcuma longa
rhizome (Curcuma longa L.), that is being recognized and used
worldwide in many different forms for its multifunctional
properties.
• In this study, extraction of curcumin, the bioactive compound of
turmeric, through Soxhlet extraction methods was investigated.
• The presence of curcumin in the extracted samples was
confirmed by Thin Layer chromatography & Paper
chromatography.
• The RF value of curcumin was found to be using Paper
chromatography and Thin layer Chromatography respectively.
11. INTRODUCTION
• Turmeric, an ancient coloring spice of Asia, as the main source of curcumin is
traditionally used for many purposes.
• Turmeric is derived from the rhizomes of Curcuma longa, which is a member of the
ginger family: Zingiberaceae
• Curcumin origin :Curcuma Longa
• Common Name : Turmeric
• Marathi Name : Halad
• Part of uses. :Dried Rhizomes
12. • The most active constituent.of Turmeric is Curcumin.
• The curcuminoids are polyphenols and are responsible for the yellow
color of turmeric.
• Curcumin, having nearly a two centuries old scientific history.The
curcumin
was firstly isolated from the turmeric in the year 1815 by the Vogel and
Pelletier. In the year
1910 Melabedzka reported structure of curcumin. Turmeric has also
been used for centuries
in Ayurvedic medicine
14. STRUCTURE OF CURCUMIN
The IUPAC name of curcumin is (1E,6E)-1,7-bis(4 hydroxy-3-methoxyphenyl)-1,6-
heptadiene3,5-dione.
The chemical formula of curcumin is C21H20O6 having molecular weight of
368.38.
The structure of curcumin states that it has aromatic ring systems containing
O-methoxy phenolic groups linked with a seven carbon linker consisting of an
alpha, beta unsaturated beta-diketone moiety.
15. EXTRACTION OF CURCUMIN
Curcumin is extracted from the dried roots of the rhizome Curcuma Longa.
Curcumin can be extracted from the following Methods :
Conventional extraction using Soxhlet. Soxhlet extractor is a
piece of laboratory apparatus invented in 1879 by Franz von Soxhlet. It was
originally designed for the extraction of a liquid from a solid materal .Typically
Soxhlet extraction is used when a desired compound as a limited solubility in a
solvent and the impurity is insoluble in that solvent.it allows for unmonitored
unmanaged operation while efficiently recycling a small amount of solvent to
dissolve a larger amount of material.
16. CHROMATOGRAPHY:
SEPARATION OF CURCUMIN FROM CURCUMINOIDS USING PAPER.
Chromatography means color-writing and the more specific definition is, it is a physical
process of separation at which a mixture of compounds can be separated and isolated,
purified into different molecules that depend on different distribution rates depending on
1.Solubility
2. Affinity (if polar or non-polar molecules)
3. Interaction with fixed material (the stationary phase, which we will define later), the
components in the mixture are dispersed between two phases, the stationary phase, and
the mobile phase, that moves at various speeds in a specified direction.
17. Any chromatographic separation technique must contain the three main parts
• 1. Sample
• 2. Mobile phase
• 3. Stationary phase.
• stationary phase: It is the solid substance at which the mixture of the components
will be separated and isolated, its nature is a solid or a liquid only.
• Mobile phase: It is a solid or liquid substance that carries a mixture composed of
a sample to be purified, isolated, separated at the surface of the stationary phase.
18. PAPER CHROMATOGRAPHY
• The technique of paper chromatography was first discovered by Synge and Martin in
1943. Among all, chromatographic techniques, paper chromatography is a type of
analytical tool which is used for separation of colored components. The principle
involved may be separation and partition of components based on their affinity towards
stationary phase.
19. ANTIBACTERIALACTIVITY OF CURCUMIN
• Curcumin is the main active component in Curcuma longa rhizome that is
being recognized and used worldwide in many different forms for its
multifunctional properties.
• A range of studies have reported the antimicrobial activity of curcumin,
including antibacterial, antiviral, and antifungal activities.
• Here, we discuss the antibacterial activity and the action mode of curcumin.
21. CONCLUSION:
• Extraction of curcumin from turmeric using Soxhlet method.
Ethanol plays a major role in Extraction process to obtain
maximumyield.The chemistry of curcumin is simple and it
has intense yellow color that changes to deep red in basic pH
solution. Curcumin have some problems like bioavailability
and solubility and these problems could be overcome with
formulations with nanoparticles which shows promising
potential to overcome this problems. Separation of
curcuminoids with TLC shows that identification of
curcuminoids in very low levels of curcuminoids in any
sample matrices is possible.
22. Paper chromatography can be continuously used for identification and separation of
various pharmaceutical components because of its low cost. Curcumin is a good
candidate for the future antibacterial drug design and it can be used along with other
potential antibacterial molecules to enhance its antibacterial efficiency. The curcumin has
good antibacterial property against different kinds of bacterial species.
23. BIBLIOGRAPHY:
• Mohamed Ahmed Sayed “A review of chromatography: Principles, Classification,
Applications” Oct-2021 Page no.2-7.
• “Indian Pharmacopoeia 2018” Page no.- 204-208 & 3799-3800.
• Mukta Gupta, Bhupinder Kapoor, Reena Gupta. “Paper Chromatography: A
Review”
• Muhammad Akram, Arslan Afzal, Ejaz Mohiuddin, Muhammad Asif. “Curcuma
longa and curcumin: a review article” Jan-2010, Page no.1-4.
• Ashok Kumar Popuri, Bangaraiah Pagala. “Extraction of curcumin from turmeric
roots” Vol-2, May-2013 page no.290-295.
24. • Surbhi Rathore, Mohammad Mukim, Pratishta Sharma, Siwani Devi, Jagdish Chandra
Nagar, Mohammad Khalid. “Curcumin: A review for health Benefits”. Vol 7, Jan 2020.
Page no. 273-275.
• Anamika Bagchi, “Extraction of Curcumin” Vol-1 Sep-Oct 2012, page no.1-3.
• Ashok Kumar Popuri, Bangaraiah Pagala. “Extraction of curcumin from turmeric roots”
Vol-2, May-2013 page no.290-295.
• Soheil Zorofchian Moghadamtousi, Habsah Abdul Kadir, Pouya Hassandarvish, Hassan
Tajik, Sazaly Abubakar, and Keivan Zandi, “A Review on Antibacterial, Antiviral, and
Antifungal Activity of Curcumin” April-2014. Page no.1-5.
• Kavirayani Indira Priyadarsini, “The chemistry of curcumin: From extraction to
Therapeutic agent” Dec 2014, Page no.20091-20093.
