Biodiesel: Renewable fuel produced from biological oils. Microalgae are known to accumulate 20-50% triglycerides (DW).  Transesterification Oil + Alcohol --> Fatty acid alkyl ester (FAME) Catalyst (Acid/ Base)

1. BIODIESEL PRODUCTION BY
SIMULTANEOUS EXTRACTION AND
CONVERSION OF TOTAL LIPIDS FROM
MICROALGAE, CYANOBACTERIA, AND
WILD MIXED-CULTURES
PAPER REVIEW
NAME : VIJENDREN KRISHNAN
SUPERVISOR : PROF. DR. YOSHIMITSU UEMURA
CO-SUPERVISOR : DR. NURLIDIA MANSOR
DATE : 22 SEPT 2014

3. INTRODUCTION
Biodiesel: Renewable fuel produced from biological
oils.
Microalgae are known to accumulate 20-50%
triglycerides (DW).
 Transesterification
Oil + Alcohol Fatty acid alkyl ester (FAME)
Catalyst (Acid/ Base)

4. EXPERIMENTAL STRAINS
Type Strains Media Source
Marine microalgae Chaetoceros gracilis Artificial seawater UTEX*
Marine microalgae Phaedactylum tricornutum Artificial seawater UTEX*
Marine microalgae Tetraselmis suecica Artificial seawater UTEX*
Freshwater
microalgae
Neochloris oleoabundans Bristol Media UTEX*
Freshwater
microalgae
Chlorella sorokiniana Bristol Media UTEX*
Cyanobacteria Synechocystis sp pcc 6803 BG -11 Hu et al., 2000
Cyanobacteria Synechocystis elongatus BG-11 Hu et al., 2000
* Culture collection of algae from the University of Texas, Austin.

5. CONVENTIONAL OIL
EXTRACTION
Lipid extracted using organic
solvent
Solvents removed by
distillation
TG transesterified

6. DIRECT TRANSESTERIFICATION
100mg lyophilized biomass
Reacted with MeOH + H2SO4
Reaction stopped by adding chloroform
Phase separation by adding water 5mL ,
centrifuged
Residual biomass recovered with 2mL
chloroform

7. ANALYSES
TOTAL LIPID ANALYSIS
TAG CONTENT DETERMINATION BY GAS
CHROMATOGRAPHY
FAME CONTENT ANALYSIS BY HAS
CHROMATOGRAPHY

8. RESULTS AND DISCUSSION

9. RESULTS AND DISCUSSION

10. RESULTS AND DISCUSSION

15. CONCLUSION
 Direct transesterification is applicable to
diatoms, green algae, cyanobacteria, and a
wild mixed culture
 High biodiesel yield.

16. THE END