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Microscopía de Rayos-X
      Tomografía 3D (BESSY)
Microscopio de rayos X
Rayos - X Blandos
                 1 - 10 nm
              (Alta resolución)


               100 - 1000 eV
             (Buena penetración)
Magnificación
Óptico   Rayos-X   SEM
¿De dónde salen los rayos X?
Microscopio de
Transmisión de rayos-X
        (TXM)
         BESSY
Tomografía 3D de células
   utilizando TXM
           BESSY
¿Por qué rayos X blandos?

                  2,34 - 4,38 nm
                   280 - 530 eV




                  CONTRASTE
                 AGUA-PROTEÍNA
Configuración del
  experimento
Análisis de los datos
Propagación lineal de los Rayos-X




      1 px = 1 proyección
LAC
Resultado
Drosophila Melanogaster
Chlamydomonas reinhardtii
Referencias
http://www.xradia.com/Technology/xraymicroscopy.html

http://xray1.physics.sunysb.edu/research/intro.php

http://en.wikipedia.org

D.Weiss, G. Schneider, B. Niemann, P. Guttmann, D. Rudolph and G. Schmahl, Ultramicroscopy 84
(2000), 185-197

G. Schneider, E. Anderson, S.Vogt, S. Knöchel, D. Weiss, M. Legros, and C. Larabell, “Computed
tomography of cryogenic cells” Surface Review and Letters 9 (2002), 177-183

G. Schneider, P. Guttmann, S. Heim, S. Rehbein, D. Eichert, B. Niemann: X-Ray Microscopy at
BESSY: From Nano-Tomography fo Fs-Imaging, SYNCHROTRON RADIATION
INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation
instrumentation (Eds.: J.-Y Choi, S. Rah), AIP Conference Proceedings 879 (2007), 1291-1294
¿Alguna pregunta...?

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