Presentation16 integrating soft skills eversole_cire

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Presentation by Dr. Pamela Eversole-Cole at Southern California Biotechnology Conference

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Presentation16 integrating soft skills eversole_cire

  1. 1. BIOTECHNOLOGY PROGRAM Biological Technology Program at Pasadena City CollegeBiological Technology Program g gy g Pasadena City College Natural Sciences Division Pamela Eversole-Cire Ph D Eversole-Cire, Ph.D. SCBC – BioLink Conference, January 13 -14, 2012
  2. 2. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Mission of the Biotechnology Program• The Mission of the Biotechnology Program is to: – prepare students, including individuals from underrepresented or special populations, f careers i research, b th i academia i l l ti for in h both in d i and in industry; – ensure that students are competitive when applying to institutions of higher education; – facilitate internships and advanced training opportunities in a variety of research fields such as stem cell biology and fields, bioinformatics; and – provide a well trained workforce to the local research community, at various educational and technical levels. 1
  3. 3. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Biotechnology Program Successes• PCC biotech students are provided with a theoretical and practical education which effectively prepares them for research and various career and educational opportunities• Students ho ha e St dents who have participated in the Biotechnology Program have Biotechnolog ha e successfully competed for employment opportunities in academic research laboratories and in the biotechnology industry• Program participants have t P ti i t h transferred to institutions of higher l f d t i tit ti f hi h learning t i to continue their education – Often after transferring, students are capable of participating in undergraduate research programs, an experience which has led to graduate school or job offers• To date, more than 150 students have been placed as interns or hires at over 50 sites including biotechnology companies, public and private institutions, and academic research facilities in the local community 2
  4. 4. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Biotechnology Program Design• The biotechnology courses are taught in a working laboratory setting – Allows the students to learn more than just core competencies and prepares the students to work in a research laboratory environment• Students are “employees” of the “PCC Biotech Company” – Follow OSHA safety standards F ll f t t d d – Staff meetings – Sign in for attendance (with time stamp) – Notebook keeping skills and proper documentation of data – Photo release forms – Confidentiality C fid ti lit agreements t protect i t ll t l property rights t to t t intellectual t i ht – Proper lab etiquette – “Hierarchy” in a research laboratory • Instructor’s role is the Principal Investigator/Research Supervisor • Teaching Assistant’s role is a p g postdoc/research scientist • Students’ roles are graduate students/lab assistants• Students are mentored and skills evaluated as if they were employed by a research institute or biotech company – S h d l d performance evaluations and negotiation f salary ( d ) Scheduled f l ti d ti ti for l (grade) 3
  5. 5. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Laboratory and Employability Skills• Students are trained to work in a research laboratory environment – Work in groups – Perform laboratory procedures and experiments – Responsible for p p p preparing own reagents and maintaining lab and g g g equipment – Learn how to document notebook properly – Participate in lab meetings• Work environment teaches student employability skills – Maintain confidentiality – Protect intellectual property rights • Notebook represents intellectual property of company – Time and resource management – How to work as part of a research team – Negotiation techniques 4
  6. 6. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Biotechnology Core Curriculum Biology 102A Bi l Biology 28 Basic Laboratory Skills Biology 20 Bioinformatics Research Biology 102B Biology 102C Advanced Techniques q Cell Culture - Mammalian DNA/Protein Biology 38 gy Biology 102D Cell and Molecular Biology Internship Stem Cell Culture Lab Biology 171C Biology 71C Physical Science 2Fluorescence Microscopy RNAi Applications Stem Cell Journal Club 5
  7. 7. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Biotechnology Core Curriculum• BIOL 102A* Introduction to fundamental laboratory skills. Basic laboratory skills techniques and use of laboratory equipment.• BIOL 102B* Advanced skills in applied biological technology. DNA and Protein Techniques. Presentation on technique/protocol (and prepare written summaries).• BIOL 102C* Advanced skills in applied biological technology. Mammalian cell pp g gy culture techniques. Projects demonstrate an application of cell culture. Requires preparation of a poster and presentation at a poster session.• BIOL 102D Advanced skills in applied biological technology. Internship in a biotechnology laboratory setting (234 hours).• BIOL 38* Cell and Molecular Biology Stem Cell Culture and Manipulation Biology. Techniques. Projects require performing directed-differentiation of mouse embryonic stem cells and evaluation of differentiated cell types. Journal Club presentations.• BIOL 28 Introduction to Bioinformatics. Structure and function of proteins and nucleic acids, molecular modeling, sequence alignment, and database management.• BIOL 71C* Exploring Topics in Biology – RNA Interference (RNAi). Biological mechanism and applications of RNAi.• BIOL 171C* Exploring Topics in Biology – Applications of Fluorescence Microscopy.• PHYSC 2 Scientific Method as Critical Thinking – Stem Cell Journal Club. * Notebook keeping skills and proper documentation of data are emphasized in all biotechnology laboratory classes at PCC to protect intellectual property rights. 6
  8. 8. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Performance Evaluations• Formal performance evaluations are performed twice each semester – Students sign up for scheduled meeting time – Need to arrive on time, be prepared, and bring required materials to meeting• PI/Supervisor reviews assignments and overall performance with student• Students negotiate with PI/supervisor for pay grade – Grade is determined by the “value” the student employee has brought to the company – Value is based on work product and overall contributions to company – Assessed on both hard (laboratory) skills and soft (employability) skills• Assessment and evaluation criteria are customized for each course and depend on the curriculum, laboratory skill set, and assignments – Assessment tools are tailored for each course – As knowledge base builds and skill set increases, expectations and evaluation criteria increase accordingly (teaches the students how to prepare for promotions in a research laboratory) 7
  9. 9. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Performance Evaluations – BIOL 102ABIOL 102A Introd ction to Fundamental Laborator Skills Introduction F ndamental Laboratory• Skills include: – Basic laboratory techniques, solution preparation, sterile technique, cloning procedures, p gp , plasmid DNA p purification, use of laboratory , y equipment, and instruction on proper notebook keeping techniques• Evaluation includes: – Midterm • Self evaluation checklist and written performance evaluation updated evaluation, resume, assignments, notebook assessment • Students are required to locate an assignment(s) in notebook using the Table of Contents and explain procedures and results in detail (simulate PI requesting information regarding experimental results) – Final • Self evaluation checklist and written performance evaluation, updated resume, notebook assessment • Routine laboratory calculations performed without reference material (simulate calculation assessment during an interview) ( ) • Grade (value) requested should be related to the student’s self evaluation 8
  10. 10. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Performance Evaluations – BIOL 102BBIOL 102B Ad anced Skills - DNA and Protein Techniq es Advanced Techniques• Skills include: – Electrophoretic techniques (PAGE), column chromatography, PCR, ELISA, DNA and protein purification, and Southern and Western Blot , p p , analyses• Evaluation includes: – Midterm • Self evaluation checklist and written performance evaluation updated evaluation, resume, assignments (written summaries), and notebook assessment • Students are asked to explain principles governing the various techniques and procedures performed (simulate an interview where the interviewer asks detailed questions regarding techniques listed on resume) – Final • Self evaluation checklist and written performance evaluation, updated resume, notebook assessment • Students are asked to explain principles governing the various techniques and procedures performed and gene expression induction experiment • Grade (value) typically related to the student’s self evaluation results 9
  11. 11. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAMPerformance Evaluations – BIOL 102CBIOL 102C Ad anced Skills - Mammalian Cell C lt re Advanced Culture• Skills include: – Basic and advanced mammalian cell culture techniques. Projects demonstrate an application of cell culture (p j pp (project p p proposal), requires ), q preparation of a poster and presentation at a poster session.• Evaluation includes: – Midterm • Self evaluation checklist and written performance evaluation evaluation, assignments, notebook assessment • Self evaluation checklist includes written explanations/calculations • Student skills are assessed using a timed practical evaluation • Peer evaluation of project p p p j proposal (simulate peer review of grant applications) – Final • Self evaluation checklist and written performance evaluation, notebook assessment • Self evaluation checklist also requires written explanations/calculations • Peer evaluation of final project presentation • Poster and poster presentation 10
  12. 12. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Performance Evaluations – BIOL 38BIOL 38 Cell and Molec lar Biolog - Stem Cell C lt re Molecular Biology CultureSkills include: – Laboratory procedures for the growth and maintenance of mouse embryonic stem (ES) cells in culture, characterization of stem cells, y ( ) , , non-directed and directed differentiation of stem cells, and genetic modification of stem cells. Projects require performing directed- differentiation of mouse ES cells and evaluation of differentiated cell types. Participation in a Journal Club.• Evaluation includes: – Midterm • Self evaluation checklist and written performance evaluation, notebook assessment • Peer evaluation of Journal Club presentation • Peer evaluation of project proposal (simulate peer review of grant applications) – Final • Self evaluation checklist and written performance evaluation, notebook p assessment • Peer evaluation of final project presentation • Grade (value) typically related to the student’s self evaluation results. 11
  13. 13. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Sample Written Evaluation FormBIOL 102A, Fall 2011 Midterm/Final EvaluationBasic Lab Skills Name:________________ Date:_________________Please write a brief statement regarding your overall “job” performance in Bio 102A,Fall 2011 (address the following areas: attendance, punctuality, teamwork, benchskills, interpersonal skills, contributions to company, notebook documentation, etc.)What do you think your “value” to the PCC Biotech Company was in Bio 102A Fall2011 (in terms of a letter grade)?Dates absent:Supervisor’s (Instructor’s) comments: 15
  14. 14. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Sample Checklist Evaluation FormMidterm/Final EvaluationName:________________Date:_________________ Exceeds Meets Needs Not Standards Standards Work ApplicableAttendancePunctualityPreparednessRespect for othersInterpersonal skillsParticipationTeamworkOverall contributions Instructor EvaluationBench Skills Skills/assignments receiving an Exceeds Standards rankingCalculations/math receive a check+ (performed superior to standard)Documentation/Notebook Skills/assignments receiving a Meets Standards rankingProject Proposal receive a check ( f i h k (performed appropriately t standard) d i t l to t d d) Skills/assignments receiving a Needs Work ranking receiveFinal Project Presentation a check- (not performed appropriately to standard) 12
  15. 15. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Sample Checklist Evaluation FormBIOL 102B Midterm/Final EvaluationDNA and Protein Techniques Name:________________ Date:_________________ Exceeds Meets Needs Not Standards Standards Work ApplicableStandard CurveELISAAffinity Column PAGE Gel drying ProcedureGFP Characterization Growth Curve Purification of GFP-hydropobic columnWestern Blot Analysis GFP induction curve-sample collection Instructor Evaluation (Notebook) Protein Assay Skills/assignments receiving an Exceeds Standards ranking Protein Standard Curve receive a check+ (performed superior to standard) Protein Concentration Calculations Skills/assignments receiving a Meets Standards ranking PAGE receive a check (performed appropriately to standard) Electroblot protein transfer Skills/assignments receiving a Needs Work ranking receive Detection a check- (not performed appropriately to standard) 13
  16. 16. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAMSample Checklist-Written Assessment Form Checklist- Biology 102C Final Evaluation Questions Biological Technology - Cell Culture Techniques Name:_______________________ Instructor: Dr. Pamela Eversole-Cire Date:________________________ Exceeds Meets Needs Not Cell Culture Equipment Standards Standards Work Applicable Laminar Flow Hood (type, function, and use) CO2 Incubator (conditions) LN2 tank Microscope (type and use) Hemocytometer (dimensions of 4x4 g ; use) y ( grid; ) Overview of Basic Cell Culture Procedures Cryopreservation of cells (procedure) Thawing of cells (procedure) Maintenance of cells in culture Written assessment evaluates students’ knowledge Data record and understanding of basic cell culture techniques. Culture vessels (sizes and types) Short responses to describe proper use of cell se Preparing medium (composition), etc. culture equipment, overview of basic cell culture Specialized Cell Culture Techniques procedures, and specialized techniques. Includes Transfection Primary culture, etc. calculations. Cell Culture Safety Precautions Disposal of waste Di l f t Simulates interview involving written assessment Contamination/decontamination,etc. Calculations and sample calculations. … 14
  17. 17. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Successful Training Components• PCC Biotechnology Program successfully prepares students t work i a Bi t h l P f ll t d t to k in research laboratory environment – Teaching biotechnology courses in manner to closely simulate the work environment in academic research laboratories and in the biotech industry – Emphasizing proper notebook keeping and documentation of data as well as signing confidentiality agreements trains a student to protect intellectual property rights – Working in groups teaches a student how to be an effective team member – Designing and performing experiments teaches a student the fundamentals of conducting research d i h – Understand laboratory responsibilities by preparing own reagents and maintaining lab and equipment – Learn time and resource management – Participation in lab related activities - meetings and journal clubs - prepares students to be a productive member of a research laboratory• Mentoring and evaluating performance and acquired laboratory skills as if students were employed by a research institute or biotech company is a key element to the successful students outcomes 16
  18. 18. BIOTECHNOLOGY Biological Technology Program at Pasadena City College PROGRAM Acknowledgements• Wendie Johnston, Ph.D. for having the foresight to design the PCC Biotechnology Program to properly prepare students to work in a research laboratory environment• David Douglass Ph D Dean of the Natural Sciences Douglass, Ph.D., Division• PCC Biotechnology Program students and participants

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