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Biotech is STEM Education
Ellyn Daugherty ellyn@BiotechEd.com www.BiotechEd.com
Biotechnology is the the application of
sc...
Every step in
biotech research
and development
integrates the
concepts,
processes and
technologies of
biology,
chemistry,
...
What a Biotech STEM Course Might Look Like
• Scientific Methodology, Lab Research, and Computer Tools
> Expt design, datab...
BS4NM, 2017
9 new/improved Molecular Modeling and
Bioinformatics Activities
• (Introducing) Computer-generated Molecular M...
Pick: “1BLI”
Biotech is STEM:
• Bioinformatics
• Databases
• Molecular modeling
Ball and Stick model - color-coded amino acids in amylase (1BLI)
Space-filling model in rainbow rendering - amylase (1BLI)
VAST can compare
two protein
structures
wild-type alpha
amylase
superimposed with
heat-resistant
engineered alpha-
amylase
Native alpha
amylase
superimposed
with heat-
resistant
engineered
alpha-amylase
See yellow
loop - due to
additions in
the ...
In this view,
you can see
that the
starch
substrate still
binds at the
active site.
See yellow
loop - due to
additions in
...
PCR =
directed
DNA
synthesis
DNA sample ID
NC_001416.1
What a Biotech STEM Curriculum Looks Like
• Scientific Methodology, Lab Research, and Computer Tools Chapters 1, 2…14
> Ex...
BiotechEd.com
PPTS, Syllabi,
Activities, Hints,
Links, etc.
gbiosciences.com
B:S4NM materials
and kits
and fishersci.com
e...
June2016 bio link_biotechisstem
June2016 bio link_biotechisstem
June2016 bio link_biotechisstem
June2016 bio link_biotechisstem
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June2016 bio link_biotechisstem

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Ellyn Daugherty presents on molecular modeling and amylase.

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June2016 bio link_biotechisstem

  1. 1. Biotech is STEM Education Ellyn Daugherty ellyn@BiotechEd.com www.BiotechEd.com Biotechnology is the the application of science, engineering, and technology to manipulate cells and their components to produce products or services that may improve the quality of human life Industrial Products Medical Products Food Products Biofuels Bioinformatics
  2. 2. Every step in biotech research and development integrates the concepts, processes and technologies of biology, chemistry, physics, mathematics, and engineering.
  3. 3. What a Biotech STEM Course Might Look Like • Scientific Methodology, Lab Research, and Computer Tools > Expt design, databases, molecular modeling, reports • Standard Lab Operating Procedures and Instruments > Solution Prep, Cell Culture > DNA/protein Isolation & Assays • Recombinant DNA Protein Production • Research/Manufacturing Applications: > Pharmaceuticals > Agricultural Products > Industrial Products > Environmental Issues > Biodefense, Food Safety > Opportunities to design protocols and products -0.1 0 0.1 0.2 0.3 0.4 0 5 10 Absorbance(a.u) Fractions (#) Ion Exchange Chromatography
  4. 4. BS4NM, 2017 9 new/improved Molecular Modeling and Bioinformatics Activities • (Introducing) Computer-generated Molecular Models • Viewing the 3-D Structure of a DNA Molecular Model • The AA Sequence Determines Structure of Insulin • Insulin: Complicated Choices for a Simple Protein • Studying Protein (Amylase) Three-Dimensionally • A Glow in the Dark Cat? What’s Next? • Who Passes the BRCA1 Test? • PCR Primer Design • What’s the Difference? Alpha or Beta, It’s All Amylase.
  5. 5. Pick: “1BLI” Biotech is STEM: • Bioinformatics • Databases • Molecular modeling
  6. 6. Ball and Stick model - color-coded amino acids in amylase (1BLI)
  7. 7. Space-filling model in rainbow rendering - amylase (1BLI)
  8. 8. VAST can compare two protein structures wild-type alpha amylase superimposed with heat-resistant engineered alpha- amylase
  9. 9. Native alpha amylase superimposed with heat- resistant engineered alpha-amylase See yellow loop - due to additions in the 1E3Z polypeptide Intended Mutation Produced using PCR
  10. 10. In this view, you can see that the starch substrate still binds at the active site. See yellow loop - due to additions in the 1E3Z polypeptide
  11. 11. PCR = directed DNA synthesis DNA sample ID NC_001416.1
  12. 12. What a Biotech STEM Curriculum Looks Like • Scientific Methodology, Lab Research, and Computer Tools Chapters 1, 2…14 > Expt design, databases, molecular modeling, reports Chapters 1, 2… • Standard Lab Operating Procedures and Instruments Chapters 1, 2… > Solution Prep, Cell Culture Chapters 3 & 4 > DNA/protein Isolation & Assays Chapters 4 & 5 • Recombinant DNA Protein Production Chapters 6-9 • Research/Manufacturing Applications: Chapters 10-14 > Pharmaceutical/Medical Biotech Chapter 12 > Agricultural Products Chapter 10 &11 > Industrial Products Chapters 1, 2, 13 > Environmental Issues Chapter 14 > Biodefense, Food Safety Chapter 14 > Opportunities to design protocols and products Chapters 1, 2…14
  13. 13. BiotechEd.com PPTS, Syllabi, Activities, Hints, Links, etc. gbiosciences.com B:S4NM materials and kits and fishersci.com emcp.com/biotech B:S4NM curriculum Biotechnology Curricular Support

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