Joan shellard biotech presentation


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Joan shellard biotech presentation

  1. 1. Biotechnology School of Health Sciences Biotech office & labs are located on the 2nd Floor Building SW9 Burnaby Campus
  2. 2. Infrastructure • BCIT Biotechnology Teaching Labs – BCIT-UBC Joint B.Sc Honour’s Degree Program • *CFI & BCKDF Funding for the Integrated Molecular Biology Lab • Genome BC & Western Economic Diversification Funding for their Technology Development Platform & Establishment of Satellite Sites *Principle Investigators: • Paula Brown (NRG) • Joan Shellard • Sarah McLeod
  3. 3. Diverse Expertise & Capabilities Main Biotech Lab • Molecular Biology • Immunology • Protein Biochemistry • Biofuel Production (Fatty Acid Biochemistry) • Animal Cell Culture • Bioprocessing (Fermentation) • Plant Biotechnology
  4. 4. Things we’re particularly good at…
  5. 5. Cell Line Engineering Dedicated Animal Cell Culture Lab containing: • 5 Level II Biological Safety Cabinets • 3 Laminar Flow Hoods • 4 CO2 Incubators • 3 Inverted Microscopes • 2 Epifluorescence Microscopes & Imaging System
  6. 6. Gene & Protein Analyses Specialized Instruments: • Multilabel Plate Reader (Envision) – used for a variety of fluorescence or absorbance-based bioassays • Real-Time PCR Machine – quantitative analysis of gene expression • Genetic Analyzer – Sanger DNA Sequencing – gene marker analysis (STRs) • Bioanalyzer – nucleic acid & protein quantification and analysis • Microarray Scanner – gene expression analysis, clinical genetics, genotyping • Microbeta Counter – used for a variety of bioassays measuring luminescence or beta-isotopes • 2D SDS-PAGE System – Proteomics
  7. 7. Plant Propagation • Plant cell culture • Seedling cultivation in dedicated growth room Greenhouse on campus…for the grown-ups!
  8. 8. Resources Dedicated Research Faculty: • Robert Forsyth: Biodiesel Production • Sarah McLeod: Cancer Immunology • Joan Shellard: Health Proteomics & Genomics Faculty Interested in (or accidentally doing) Applied Research: • Navkiran Gill : Host – Pathogen Interactions • Keith Turner: Plant Biotechnology
  9. 9. High Energy Resources Our Biotech Students! Funded by: • BCIT Student Employment Program • PI-funded summer Co-ops • NSERC Undergrad Student Research Awards Role: To assist Faculty in their applied research projects
  10. 10. Sample of Research Projects PI: Rob Forsyth Project: Production of biodiesel that is compatible with conventional engines & cold weather climates Funding: BCIT Professional Development (PD) Funds Status: • Pilot experiments complete • Patent application submitted to ARLO for review • Scale-up & validation underway
  11. 11. Accidental Fame! Plant Man: Keith Turner Project: Restoration of cherry trees from rare cultivars (cuttings) Collaborator: Douglas Justice, UBC Botanical Gardens Students: entire 1st Year Biotech class, as part of their plant lab Status: Baby trees growing in test tubes …
  12. 12. PI-Led Research Projects PI: Sarah McLeod Project: Role of the proteins YKL-40 and YKL-39 in interactions between cancer cells and tumourassociated macrophage cells Funding: BCIT Institutional Research Fund (awarded by peer review) & PD funds Students: Jordan McGarry (BSEP), Aisha Ghani (Summer Co-op) Status: Preliminary data have been obtained and are informing ongoing experiments. A manuscript and grant application will be submitted in 2014
  13. 13. Collaborative Research Projects PI: Joan Shellard Project : Generation of antibodies for the selective capture of serum biomarkers and identification by Mass Spectrometry Funding: BC Proteomics Network Small Projects Grant/Michael Smith Health Research Fund Collaborators: Suzanne Perry, Michael Smith Labs (CHiBi), UBC and Prostate Centre, VGH Status: Antibodies have been generated and used in immunoprecipitations of high versus low PSA plasma samples. Mass Spec analysis underway. Project: Identification of disease susceptibility markers by comparing whole genomes from selected human subpopulations Funding: CFI (computer workstation), PD funds Collaborators: Karen Mooder (BCIT MedLab), David Holloway (BCIT Mathematics) Student: Elizabeth Dai (BSEP) Status: Ongoing
  14. 14. Industry Projects Company: iProgen Biotech, Richmond BC PIs: Joan Shellard & Sarah McLeod Research Associate: Lindsay Gung Project: Generation of cell lines for the production of protein transduction mediator (PTM)tagged fusion proteins Funding: NSERC Engage Grant Status: Completed Company: Novation Pharma, Burnaby BC PIs: Sarah McLeod & Joan Shellard Project: Investigation into the pathway(s) regulating targeted mRNA instability Funding: NSERC Engage grant app contemplated Status: Planning phase
  15. 15. Model For Research In the Schools A Work in Progress…. School of Health has a great talent pool and a diversity of expertise, which allows for innovative cross-disciplanary research Challenges: • Lack of critical mass in any ‘’one”’ area • Small # of faculty pursuing applied research….and we teach! Best Approach? Collaboration and involvement of student “hands” $ Funding: Curriculum: Tie applied research projects to student curricula Challenges: Research must fit into a tidy student project Grants: BCIT has “university” status wrt Tri-Cuncil funding (NSERC, CIHR) Challenges: We are small fish in a big competitive pond and don’t have grad students
  16. 16. Industry Collaborations Strengths: • BCIT has a great track record of working with industry • Collaborations with industry seem much more flexible than universities, particularly wrt IP policy and NDAs Funding Challenges: • Biotechnology companies typically operate without cash flow and do not fund external research (they will hire CROs) • Pharma has money but you have to pitch “what you can do for them”, which means having preliminary data to approach them Best Approach? • Industry-Academic Grants (govt susidizes THEIR research ; ) • PI-Led projects using small project grants and/or BCIT seed funds
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