ISEV2014 - Introduction to Pathogen Derived EV's (H. Del Portillo)
1. PATHOGEN-‐DERIVED
EVs
CHAIRS
ANTONIO
MARCILLA
Departament
de
Biologia
Cel.lular
i
Parasitologia,
Universitat
de
València,
Valencia,
Spain
antonio.marcilla@uv.es
HERNANDO
A
DEL
PORTILLO
ICREA
at
Barcelona
Centre
for
InternaJonal
Health
Research
–
CRESIB,
Barcelona,
Spain
hernandoa.delporJllo@cresib.cat
ISEV
2014,
RoRerdam
April
30th
–
May
3rd
2014
2. O1C-‐013
TRYPANOSOMA
CRUZI-‐DERIVED
MICROVESICLES
TRIGGER
DISTINCT
STRAIN-‐SPECIFIC
PROINFLAMMATORY
ACTIVITY
VIA
TLR2
R.
Soares
1,*
K.
Ribeiro
2,
C.
Miranda
2,
P.
Nogueira
1,
A.
C.
Silveira
1,
O.
MarKns-‐Filho
1,
A.
C.
Torrecilhas
2
1Laboratório
de
Biomarcadores
de
DiagnósKco
e
Monitoração,
Centro
de
Pesquisas
René
Rachou,
Belo
Horizonte,
2Departamento
de
Ciências
Biológicas,
Universidade
Federal
de
São
Paulo,
Diadema,
Brazil
O1C-‐014
Biogenesis
Mechanisms
of
Bacterial
Vesicles
M.
Kuehn
1,*
1Biochemistry,
Duke
University
Medical
Center,
Durham,
United
States
O1C-‐015
Membrane
vesicles
released
from
Uropathogenic
Escherichia
coli
transport
an
RNA
cargo
C.
Blenkiron
1,*
,
D.
Simonov
1,
A.
MUTHUKARUPPAN
1,
P.
Tsai
1,
S.
Green
1,
C.
Print
1,
S.
Swi`
1,
A.
Phillips
1
1Faculty
of
Medical
and
Health
Sciences,
University
of
Auckland,
Auckland,
New
Zealand
O1C-‐016
Extracellular
vesicle-‐mimeKc
nanovesicles
derived
from
bacterial
protoplast
as
next
generaKon
vaccine
delivery
system
for
effecKve
prevenKon
of
infecKous
diseases
O.
Y.
Kim
1,*
,
S.
J.
Choi
1,
K.-‐S.
Park
1,
S.
C.
Jang
1,
J.
Lötvall
2,
Y.-‐K.
Kim
1,
Y.
S.
Gho
1
1Life
Science,
POSTECH,
Po
Hang,
Korea,
Republic
Of,
2Kre`ing
Research
Centre,
Internal
Medicine,
University
of
Gothenburg,
Gothenburg,
Sweden
O1C-‐017
ConservaKon
of
exosome
funcKon
during
viral
infecKon
in
Drosophila
melanogaster
C.
Kerr
1,*
,
L.
Foster
1,
E.
Jan
1
1Biochemistry
and
Molecular
Biology,
University
of
BriKsh
Columbia,
Vancouver,
Canada
4. “SomeJme,
about
150,000
years
ago,
Homo
sapiens
emerged
in
eastern
Africa
and
spread
throughout
the
world,
possibly
in
several
waves,
unJl
15,000
years
ago.
At
the
end
of
the
Ice
Age
humans
had
migrated
to
and
inhabited
virtually
the
whole
of
the
face
of
the
Earth,
bringing
some
parasites
with
them
and
collecJng
others
on
the
way.
During
our
relaJvely
short
history
on
Earth,
humans
have
acquired
an
amazing
number
of
parasites,
about
300
species
of
helminth
worms
and
over
70
species
of
protozoa
.
Many
of
these
are
rare
and
accidental
parasites,
but
we
sJll
harbor
about
90
relaJvely
common
species,
of
which
a
small
proporJon
cause
some
of
the
most
important
diseases
in
the
world.
So
vast
is
the
field
of
human
parasitology,
and
so
many
and
far-‐reaching
the
discoveries
made,
that
it
is
not
possible
to
do
jusJce
to
the
whole
subject.
Therefore;
only
the
most
significant
aspects
and
the
most
important
parasites
are
considered
under
two
major
headings,
the
helminth
worms
and
the
protozoa”.
COX
F.E.G.
CLINICAL
MICROBIOLOGY
REVIEWS,
Oct.
2002,
p.
595–612
HISTORY
OF
HUMAN
PARASITOLOGY
7. Hotez
et
al.
2008
J
Clin
Inv
Worms
are
responsible
for
millions
of
clinical
cases
8. Threadgold,
1963
Quart.
J.
micr.
Sci.
FIG.
3
(plate),
A,
cuJcular
surface,
showing
the
electron-‐dense
zone
of
invaginaJons,
pinocytoJc
vacuoles,
and
small
vesicles.
9.
10. E.
caproni
100
nm
100
nm
F.
hepa.ca
56
%
SECRETOME
ECV
In
summary,
although
the
secreJon
of
exosome-‐like
vesicles
has
been
demonstrated
in
several
organisms,
we
have
shown
the
producJon
of
these
structures
by
parasiJc
helminths
for
the
first
Jme.
12. PARASITIC
PROTOZOA
AND
ASSOCIATED
DISEASES
hRp://www.pathobio.sdu.edu.cn/sdjsc/engparabook/ch077.htm
13. www.stanford.edu
Trichomona
vaginalis
is
the
causaKve
agent
of
trichomoniasis,
and
is
the
most
common
pathogenic
protozoan
infecKon
of
humans
in
industrialized
countries
The
WHO
has
esKmated
that
275
million
cases
of
infecKon
are
acquired
annually
worldwide.
14.
15. PreincubaKon
with
exosomes
of
a
highly
adherent
strain
increases
adherence
of
a
poorly
adherent
strain
to
Ects
21. P.
falciparum
216
million
clinical
cases
650.000
deaths
P.
vivax
2.85
billions
of
people
at
risk
70-‐320
millions
of
clinical
cases
yearly
Global
distribuKon
and
endemicity
of
P.
falciparum
&
P.
vivax
27. Exosomes
Hypothesis: - Exosomes derived from Plasmodium vivax infected reticulocytes
contain parasite proteins and can modulate immune responses.
exosomes
MVB
Aikawa,
Barnwell,
Galinski.
del
PorKllo
et
al
2001
Nature
28. Exosomes+CpG immunization
Py XL Py XLPy XL
Immunized
mice
present
sterile
protecJon
in
subsequent
infecJons
20 days 20 days
parasitemia
P. yoelii 17XL
NI
5 µg s.c.10 µg s.c
+CpG. rexPy
rexC
rexPy
rexC
MarKn-‐Jaular
et
al.,
2011
PLoS
One
29. PARASITES
AND
EVs
Barteneva
et
al.,
2013
FronKers
Cell
Inf
Microbiol
