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DNA, RNA, and Protein Synthesis
Importance and Structure of DNA:  Deoxyribo-Nucleic Acid ,[object Object],[object Object],[object Object]
Figure 16.2a  The Hershey-Chase experiment: phages
Figure 16.2b  The Hershey-Chase experiment
Phages Infecting a bacterium
Figure 16.1  Transformation of bacteria – Griffith (and later Avery, McCarty and MacLeod)
The Structure of DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Structure of DNA cont’ ,[object Object],[object Object],[object Object]
Beginning of the 1950’s several labs were studying the structure of DNA ,[object Object],[object Object],[object Object]
Figure 16.4  Rosalind Franklin and her X-ray diffraction photo of DNA
Watson and Crick
April 1953 – Classical one page paper in  Nature  by Watson and Crick ,[object Object],[object Object],[object Object],[object Object]
Figure 16.5  The double helix
Internal Structure of DNA:  Purine and pyridimine?  REMEMBER X-RAY DATA
Confirms Erwin Chargaff’s Rules  ,[object Object],[object Object],[object Object],[object Object]
Information storage in DNA ,[object Object],[object Object],[object Object]
Replication/Duplication of DNA ,[object Object],[object Object],[object Object]
Figure 16.7  A model for DNA replication: the basic concept (Layer 1)
Figure 16.7  A model for DNA replication: the basic concept (Layer 2)
Figure 16.7  A model for DNA replication: the basic concept (Layer 3)
Figure 16.7  A model for DNA replication: the basic concept (Layer 4)
Figure 16.8  Three alternative models of DNA replication
Figure 16.9  The Meselson-Stahl experiment tested three models of DNA replication (Layer 1)
Figure 16.9  The Meselson-Stahl experiment tested three models of DNA replication (Layer 2)
Figure 16.9  The Meselson-Stahl experiment tested three models of DNA replication (Layer 3)
Figure 16.9  The Meselson-Stahl experiment tested three models of DNA replication (Layer 4)
There are a series of enzymes that control DNA replication – enzymes which: ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA REPLICATION
Figure 16.10  Origins of replication in eukaryotes
Figure 16.11  Incorporation of a nucleotide into a DNA strand
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Antiparallel Arrangement of Double Strands
Definitions ,[object Object],[object Object],[object Object],[object Object]
More Definitions ,[object Object],[object Object],[object Object]
Definitions Cont’ ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA REPLICATION -VIDEO
Figure 16.13  Synthesis of leading and lagging strands during DNA replication
Figure 16.14  Priming DNA synthesis with RNA
Figure 16.15  The main proteins of DNA replication and their functions
Figure 16.16  A summary of DNA replication
Figure 16.17  Nucleotide excision repair of DNA damage
A PROBLEM! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Prokaryotes have circular DNA – no problem at ends (there aren’t ANY! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Figure 16.19a  Telomeres and telomerase: Telomeres of mouse chromosomes
Ribonucleic Acid (RNA) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Synthesis of RNA - transcription ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Figure 17.2  Overview: the roles of transcription and translation in the flow of genetic information (Layer 1)
Figure 17.2  Overview: the roles of transcription and translation in the flow of genetic information (Layer 2)
Figure 17.2  Overview: the roles of transcription and translation in the flow of genetic information (Layer 3)
Figure 17.2  Overview: the roles of transcription and translation in the flow of genetic information (Layer 4)
Figure 17.2  Overview: the roles of transcription and translation in the flow of genetic information (Layer 5)
Figure 17.6  The stages of transcription: initiation, elongation, and termination (Layer 1)
Figure 17.6  The stages of transcription: initiation, elongation, and termination (Layer 2)
Figure 17.6  The stages of transcription: initiation, elongation, and termination (Layer 3)
Figure 17.6  The stages of transcription: initiation, elongation, and termination (Layer 4)
Figure 17.6  The stages of transcription: elongation
Three types of RNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Three types of RNA
Protein Synthesis = Translation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Ribosomes bring mRNA together with amino acid bearing tRNA’s ,[object Object],[object Object],[object Object],[object Object]
Figure 17.15  Translation – the basic concept
Preparation of Eukaryotic mRNA ,[object Object],[object Object],[object Object]
 
Figure 17.17  The initiation of translation
Figure 17.18  The elongation cycle of translation
Protein Synthesis (cont’) Initiation – elongation - termination ,[object Object],[object Object],[object Object],[object Object]
Figure 17.4  The dictionary of the genetic code
Figure 17.3  The triplet code
tRNA complexes with its amino acid in the cytoplasm using ATP – activated tRNA ,[object Object],[object Object]
The first tRNA and its amino acid now occupy the P site of the large ribosomal subunit ,[object Object],[object Object]
Protein Synthesis (continued) ,[object Object],[object Object],[object Object],[object Object]
Translocation – the ribosome moves the tRNA into the A site, and its attached peptide to the P site, as the previous tRNA from the P site moves to the E (Exit) site and leaves the ribosome ,[object Object]
Yet More Protein Synthesis ,[object Object],[object Object],[object Object]
A question? ,[object Object]
The Answer! ,[object Object],[object Object],[object Object],[object Object]
FINALLY - SUMMARY ,[object Object],[object Object]
Figure 17.17  The initiation of translation
Figure 17.18  The elongation cycle of translation
Figure 17.19  The termination of translation
Figure 17.20  Polyribosomes
Table 17.1  Types of RNA in a Eukaryotic Cell
Figure 17.23  The molecular basis of sickle-cell disease: a point mutation
Figure 17.24  Categories and consequences of point mutations: Base-pair insertion or deletion
Figure 17.24  Categories and consequences of point mutations: Base-pair substitution
Figure 17.25  A summary of transcription and translation in a eukaryotic cell
Figure 18.19  Regulation of a metabolic pathway
Control of Protein Synthesis Regulation of Gene Expression ,[object Object],[object Object],[object Object],[object Object],[object Object]
Regions of the Operon (DNA) ,[object Object],[object Object],[object Object],[object Object],[object Object]
Figure 18.20a  The  trp  operon: REPRESSIBLE
Figure 18.21a  The  lac  operon: INDUCIBLE
Figure 19.7  Opportunities for the control of gene expression in eukaryotic cells

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5 DNA RNA Protein Synthesis

  • 1. DNA, RNA, and Protein Synthesis
  • 2.
  • 3. Figure 16.2a The Hershey-Chase experiment: phages
  • 4. Figure 16.2b The Hershey-Chase experiment
  • 5. Phages Infecting a bacterium
  • 6. Figure 16.1 Transformation of bacteria – Griffith (and later Avery, McCarty and MacLeod)
  • 7.
  • 8.
  • 9.
  • 10. Figure 16.4 Rosalind Franklin and her X-ray diffraction photo of DNA
  • 12.
  • 13. Figure 16.5 The double helix
  • 14. Internal Structure of DNA: Purine and pyridimine? REMEMBER X-RAY DATA
  • 15.
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  • 17.
  • 18. Figure 16.7 A model for DNA replication: the basic concept (Layer 1)
  • 19. Figure 16.7 A model for DNA replication: the basic concept (Layer 2)
  • 20. Figure 16.7 A model for DNA replication: the basic concept (Layer 3)
  • 21. Figure 16.7 A model for DNA replication: the basic concept (Layer 4)
  • 22. Figure 16.8 Three alternative models of DNA replication
  • 23. Figure 16.9 The Meselson-Stahl experiment tested three models of DNA replication (Layer 1)
  • 24. Figure 16.9 The Meselson-Stahl experiment tested three models of DNA replication (Layer 2)
  • 25. Figure 16.9 The Meselson-Stahl experiment tested three models of DNA replication (Layer 3)
  • 26. Figure 16.9 The Meselson-Stahl experiment tested three models of DNA replication (Layer 4)
  • 27.
  • 29. Figure 16.10 Origins of replication in eukaryotes
  • 30. Figure 16.11 Incorporation of a nucleotide into a DNA strand
  • 31.
  • 32.
  • 33.
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  • 36. Figure 16.13 Synthesis of leading and lagging strands during DNA replication
  • 37. Figure 16.14 Priming DNA synthesis with RNA
  • 38. Figure 16.15 The main proteins of DNA replication and their functions
  • 39. Figure 16.16 A summary of DNA replication
  • 40. Figure 16.17 Nucleotide excision repair of DNA damage
  • 41.
  • 42.
  • 43. Figure 16.19a Telomeres and telomerase: Telomeres of mouse chromosomes
  • 44.
  • 45.
  • 46. Figure 17.2 Overview: the roles of transcription and translation in the flow of genetic information (Layer 1)
  • 47. Figure 17.2 Overview: the roles of transcription and translation in the flow of genetic information (Layer 2)
  • 48. Figure 17.2 Overview: the roles of transcription and translation in the flow of genetic information (Layer 3)
  • 49. Figure 17.2 Overview: the roles of transcription and translation in the flow of genetic information (Layer 4)
  • 50. Figure 17.2 Overview: the roles of transcription and translation in the flow of genetic information (Layer 5)
  • 51. Figure 17.6 The stages of transcription: initiation, elongation, and termination (Layer 1)
  • 52. Figure 17.6 The stages of transcription: initiation, elongation, and termination (Layer 2)
  • 53. Figure 17.6 The stages of transcription: initiation, elongation, and termination (Layer 3)
  • 54. Figure 17.6 The stages of transcription: initiation, elongation, and termination (Layer 4)
  • 55. Figure 17.6 The stages of transcription: elongation
  • 56.
  • 58.
  • 59.
  • 60. Figure 17.15 Translation – the basic concept
  • 61.
  • 62.  
  • 63. Figure 17.17 The initiation of translation
  • 64. Figure 17.18 The elongation cycle of translation
  • 65.
  • 66. Figure 17.4 The dictionary of the genetic code
  • 67. Figure 17.3 The triplet code
  • 68.
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  • 70.
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  • 72.
  • 73.
  • 74.
  • 75.
  • 76. Figure 17.17 The initiation of translation
  • 77. Figure 17.18 The elongation cycle of translation
  • 78. Figure 17.19 The termination of translation
  • 79. Figure 17.20 Polyribosomes
  • 80. Table 17.1 Types of RNA in a Eukaryotic Cell
  • 81. Figure 17.23 The molecular basis of sickle-cell disease: a point mutation
  • 82. Figure 17.24 Categories and consequences of point mutations: Base-pair insertion or deletion
  • 83. Figure 17.24 Categories and consequences of point mutations: Base-pair substitution
  • 84. Figure 17.25 A summary of transcription and translation in a eukaryotic cell
  • 85. Figure 18.19 Regulation of a metabolic pathway
  • 86.
  • 87.
  • 88. Figure 18.20a The trp operon: REPRESSIBLE
  • 89. Figure 18.21a The lac operon: INDUCIBLE
  • 90. Figure 19.7 Opportunities for the control of gene expression in eukaryotic cells