Mise en scene refers to everything presented within the frame of a film, including settings, costumes, lighting, and positioning of characters and objects. These elements are deliberately chosen by filmmakers to convey meaning and influence audience perception. For example, a tree can be made to look threatening through lighting, weather, and added elements, or non-threatening by showing children playing beneath it on a sunny day. Filmmakers also use mise en scene elements like settings, costumes, lighting, and character positioning to establish genre expectations and mood, provide clues about characters, and guide audience attention within a scene.