Disease suppressive soil has both diverse and uniform ecology : Modeling and characterization
•Download as ODP, PDF•
1 like•1,164 views
SIGBIO28
Recommended
Recommended
More Related Content
What's hot
What's hot (15)
Similar to Disease suppressive soil has both diverse and uniform ecology : Modeling and characterization
Similar to Disease suppressive soil has both diverse and uniform ecology : Modeling and characterization (20)
More from Y-h Taguchi
More from Y-h Taguchi (20)
Disease suppressive soil has both diverse and uniform ecology : Modeling and characterization
- 1. Disease suppressive soil has both diverse and uniform ecology : Modeling and characterization Y-h. Taguchi, Chuo University, Kazunari Yokoyam a, NARO sector １ sector ２ sector ３ disease rate disease rate disease rate 19.2％ 69.2％ 100％
- 2. 0. Introduction (so far, long hostory over m ore than 20 years ...) replant failure No cultivations without disinfect
- 3. random sampled dilute colony to biolog suspension agar plate plate Soil Sample culture Step B Step A Cluster Analysis step C digitized carbon resource plate reader consumption ability Experimental procedure of biodiversity index method for soil bacteria
- 4. Basically,MicroPlate GN carbon resources
- 5. Appearance of biolog plate coloring T bacteria consum e his this carbon resources Bacteria 1 Bacteria 2 Bacteria 3 Bacteria 4
- 6. Estim ation of biodiversity by (Yokoyama 1992) biolog plate Colony method S bacteria is isolated by diluted plate m ethod and oil 95 carbon resources consum ption ability is used for the estim ation of biodiversity distance A- B bacteria A bacteria B distance AB -C bacteria C distance ABC -D bacteria D biodiversity independent of taxon = Squared total cluster distance / num ber of bacteria
- 7. Many kinds of species are in suppressive soil biodiversity index 多 様 性 指 数 replant failure （％） An exam ple of spinach repeat failure (Hida, Gifu)
- 8. dilute suspension dilute suspension to soil sample biolog plate Skip ステッ プC プレー 有機物分解能を数 ト 値化 リー ダー Experimental Procedure of soil bacteria biodiversity/activity measurement
- 9. OmniLog Robot eye automatically measure carbon resources consumption rate and put into data base low biologically activity soil High biologically activity soil Carbon resource consumption rates in soil are measured over 48 hours
- 10. Cottony Leak of Scarlet Runner Bean (Phaseolus coccineus:ベニバナインゲン) and soil bacteria biodiversity/activity Cottony Leak, which causes repeat failure, can be suppressive by the soil with high biodiversity/activity Sector １ Sector ２ Sector ３ disease rate（％） 19.2 < 69.2 < 100 soil bacteria 1,114,100 > biodiversity/activity（＊） 412,000 > 306,097 sector １ sector ２ sector ３ disease rate disease rate disease rate 19.2％ 69.2％ 100％ Katakura Chikkarin CO. LT T D, sukuba Research Institute Co.,Ltd. 、 Agricultural Ree h sarc I ns , I baraki Agric titute ultural Ce r, DGC Technology Inc. nte 10 （＊） Integration of time development
- 11. １.Model Assum ptions Rapidly consum ed are consum ed by ⇔ carbon resources m ore kinds of soil bacteria Slowly consum ed are consum ed by ⇔ carbon resources less kinds of soil bacteria Consum ption Carbon resource A Carbon resource B consum e Soil bacteria T e im
- 12. Carbon resources vs S Bacteria : Interaction Matrix oil Office 3.3 # of carbon resources consum ed by vel:2 at least a bacteria S Bacteria oil Carbon Resources The ｉ２ th species soil bacteria does not consum e carbon resource ｊ２ The ｉ1 th species soil bacteria consum es carbon resource ｊ1 Interactive region
- 13. Model ：Lotka-Volterra type M0 dx i N i =(Σ y )x i j=1 j dt d yj N =−(Σ N x i )y j , j⩽M0 dt i= M j 0 0, j> M0 xi : Population of the i th species bacteria yj: T am ount of unconsum ed ｊ th carbon resources he
- 14. Initial condition: xi=0, yj=1 -ε 、（ε：random num ber、0 < ε < 0.1) M（# of kinds of carbon resources）９０ N（# of species of soil bacrteria）９０ M0 =20
- 15. M0=４0 M0=８0
- 16. :(fig1c.eps) S ator:(ImageMagick) ick Title:(fig1b.eps) Creator:(ImageMagick) Healthy Title:(fig1a.eps) Creator:(ImageMagick) ationDate:(2011-11-13T14:00:59+09:00) CreationDate:(2011-11-25T15:50:53+09:00) CreationDate:(2011-11-25T15:50:48+09:00) guageLevel:1 LanguageLevel:1 LanguageLevel:1 disease disease disease rate： rate： rate： １０ 69.2％ １9.2％ ０％ M0=20 M0=４0 M0 =８0
- 17. Actual m easurem ent（？） of disease supressive soil （carbon resources consum ption rate of each soil bacteria: colonized and isolated well ） S bacteria ID oil Carbon Resource ID
- 18. Conclusion ・We have developed a m odel reproducing carbon resource consum ption rate tim e developm ent of soil bacteria. ・Disease suppression is suggested to be enabled bu diverse carbon resources consum ption rates. ・It does not disagree with the actual m easurem ents of interaction m atrix. Furtherm ore.. （om itted, see the next slide） ・ nMDS em bedding exhibits that hom ogeneity and uniform ity of tim e developm ent are
- 19. △disease rate：１０ specialist ＞ generalist ０％ ＋disease rate：６９． ２％ ◯disease rate ：１９． specialist ＝ generalist ２％ post ☓com specialist ＜generalist points＝carbon resources specialists generalist （specialized to a (not specialized) few carbon resources）
Editor's Notes
- これは、それぞれのウェルに塗られている炭素源です。高分子、エステル、糖、アミノ酸、有機酸他、全体で９種類のカテゴリーから、細菌の種類を分類するために有効な炭素源が選抜されています。このプレートは世界中で同一の品質の物を手に入れることが出来るため、世界中の微生物研究者が使用しており、そのため結果をお互いに比較することができる良さがあります。
- 未知の細菌をバイオログプレートに分注すると、この様に様々なパターが得られます。このパターは２の９５乗、これは１０の２９乗の組み合わせを表現することが出来、地球上の細菌を全て網羅できる物と考えられています。