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Cambrige Jan2009 1

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Cambrige Jan2009 1

  1. 1. MICROME J. Tamames, V. de Lorenzo et al Environmental Microbiology Laboratory CNB-CSIC, Madrid, Spain
  2. 2. Main goals:  Understanding how bacteria which inhabit natural niches sense and process multiple environmental signals into distinct transcriptional responses –both at the level of single cells and as a community.  Exploring the possibility of programming bacteria for deliberate environmental release aimed at biodegradation of toxic pollutants or as biosensors for monitoring the presence of given chemicals.  Developing tools for the prediction of metabolic outcomes by simulation of single cell or community metabolism Molecular Environmental Microbiology Laboratory Centro Nacional de Biotecnología-National Center of Biotechnology, Madrid, Spain Head: Dr. Victor de Lorenzo
  3. 3. Genetic Traps Mining biodegradative activities out of complex metagenomes, by coupling the rise of the reaction products to transcriptional regulators that activate expression of a marker or reporter gene. Ring monooxygenases: DBT desulphurization:  -HCH dehydrochlorination: Biosensor strains against explosives present in antipersonnel landmines We employ our genetic platforms for evolving transcriptional regulators responsive to residues of explosives Ongoing research projects +DNT
  4. 4. Refactoring a minimal TOL plasmid. de-construction and re-construction the TOL pathway and its regulatory elements encoded in the pWW0 plasmid of Pseudomonas putida mt-2. Ongoing research projects Bioremediation and biosensing of arsenic pollution. Synthetic Biology approach for the design of bacterial strains capable of responding differently to the presence of low and high concentration of arsenic in the medium. Orthogonalization of metabolic modules. The Proteomic Switch: a new strategy to bring about conditional proteomic knockouts in E. coli
  5. 5. Ongoing research projects Logic circuit analysis of the TOL circuit of P. putida
  6. 6. Predicting biodegradation Gomez et al. Mol Sys Biol (2007)‏ Acetaldehyde Solubility: 0.1 g/100 ml MW: 44 D Atomic triads: C-C-H 4 C-C=O 1 H-C-H 3 H-C=O 1 Learning machine C C O H H H H
  7. 7. Environmental and taxonomic diversity Aerial Animal host Artificial Freshwater sediment Freshwater Freshwaters-Saline waters Geothermal Gut Hydrothermal Marine host Oil Oral Other tissue Plants Saline sediment Saline waters Soil Soil-Freshwaters interfase Soil-Saline waters interfase Vagina
  8. 8. http://metagenomics.uv.es/envDB EnvDB database For the study of environmental and taxonomic diversity
  9. 9. GROUP LEADER PROF. VÍCTOR DE LORENZO POSTDOCTORAL BELÉN CALLES AITOR DE LAS HERAS JOSE IGNACIO JIMÉNEZ ESTEBAN MARTÍNEZ JAVIER TAMAMES PREDOCTORAL ALEJANDRO ARCE MAX CHAVARRÍA RAFAEL SILVA-ROCHA TECHNICIANS SOFÍA FRAILE

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