histopathology slides

1. Histopathology slide of –
Typhoid Ulcer ilem
Tuberculous Lymphadenitis
Amoebic colitis
Dr. Sandeep singh
GMC

2. Typhoid Ulcer
• Acute infection caused by Salmonella typhi.
• Ingested through contaminated food and water.
• Characteristic – Rise in temperature ,
rose spots on skin.

3. Grossly
• Ileum is affected most often
• Peyer’s Patches show oval typhoid ulcer with
their long axis along with length of the bowel.
• Base of the ulcer is black due to sloughed
mucosa ,
• margin is raised
• and cellular proliferation.

5. Microcopically
• Hyperaemia, oedema and
cellular proliferation
consisting of phagocytic
histiocytes , lymphocytes
and plasma cells

7. Tuberculous lymphadenitis
• The most common form of tuberculosis
infections that appears outside the lung
• It is always secondary
• G/A The lymph nodes are enlarged and are
matted together due to periadenitis.
• The cut surface in the tuberculous areas is
yellow, cheesy, opaque and caseous while
elsewhere is grey brown

8. Caseating granulomatous lymphadenitis. A, Cut section of matted
mass of lymph nodes shows merging capsules and large areas of

9. M/E
• The caseous areas show nuclear debris
• The periphery of caseous foci shows
granulomatous inflammation consisting of
epithelioid cells, surrounded by lymphocytes
and some plasma cells.
• Epithelioid cells may fuse to form giant cells in
the granuloma and may have nuclear
arrangement at the periphery of the cell
(Langhans’ giant cell) or the nuclei may be
distributed haphazardly (foreign body giant cell)

10. Caseating epithelioid cell granulomas with some Langhans’ giant cells in the cortex
of lymph node.

11. Tuberculous lymphadenitis. Characteristic sliper-shaped epithelioid cells, minute
caseation necrosis and Langhans’ giant cells.

12. Tuberculous lymphadenitis. Large areas of caseation necrosis with
dystrophic calcification, surrounded by epithelioid cells. Inbox shows AFB.

13. Amoebic colitis
• DUE to Entamoeba histolytica affect large
intestine – Flask shaped ulcer
• Gross-
• amebic colitis. Several nodular lesions, slightly
elevated areas of the mucosa with irregular
necrotic centers in advance cases flask shapped
ulcer narrow neck and broad base.

15. Microscopically
• Ulcerated area showing chronic inflammatory
reaction consisting of lymphocytes , plasma
cells, macrophages and eosinophils .
Trophozoites of entamoeba are seen in
inflammatory exudate with oedema and vascular
congestion.