TM Media products Catalog 2018 | Products List

PRODUCT LIST
2018-19
PRODUCT LIST
2018-19
DEHYDRATED CULTURE MEDIA
BIOLOGICAL MEDIA BASES
MOLECULAR BIO GRADE CHEMICALS
ANTIBIOTIC SENSITIVITY DISC
PLANT TISSUE CULTURE MEDIA
TMmedia

PRODUCT LIST
2018-19
Titan Biotech
Manufacturer & Exporter
|ISO 9001:2015 ISO 13485:2016 ISO 22000:2005 FSSAI
cGMP CE Certified
|ISO 11133:2014
|
| |
CORPORATE OFFICE
th
903-909, 9 Floor, Bigjos Tower, Netaji Subhash Place, Delhi-110034, India
Tel : +91-11-27355742, 71239900
R. O. & WORKS
Unit I : A-902 A, RIICO Industrial Area, Phase-III, Bhiwadi-301019, Rajasthan, India
Unit II : E-540, Industrial Area, Chopanki, Bhiwadi-301707, (Raj.), India
Domestic Enquiries
marketing@titanbiotechltd.com
Export Enquiries
export@titanbiotechltd.com
MAIL IDs
www.amazon.in|www.mymicrolab.com
FOR ONLINE SHOPPING

GENERAL TERMS
AND CONDITIONS
A team of experts are dedicated to provide the highest levels of technical support and customer
service. The Technical service department is available to answer questions about products. Along with it
our technical sales representative is equipped with knowledge for product selection and evaluation.
The foremost mandatory point is that the order must be complete with TM Product Code, Product Name,
Pack Size and Price. In case the adequate information is not shared, the order will not be processed
further. Orders, once placed, will not be edited or cancelled. Orders placed with us for execution, may be
supplied directly through our stockist which is company's prerogative.
All correspondence related to Purchase Orders should be done at our Headquarters (Delhi, India).
st
The price mentioned in this list are applicable from 1 July 2018. No previous price list is applicable. This
price list to be treated for further order and confirm these prices when placing an order. The prices are
subject to change without prior notice. With mutual consent, the order will be finalised in case of change
in price. All the amendments for the year 2018-19 will be updated on website www.tmmedia.in/
product-catalogue
Good and Service Tax (GST) is applicable on the products above the prices mentioned. State Road
Permit/ E-Way Bills is also essential (if applicable), without which order despatch will not be processed.
In case of any exemptions from GST, the certificate must be shared with the order otherwise GST will be
charged extra as applicable.
Our GST No. is 08AAACT0078J1ZA.
The orders for Culture Media and Media Bases are accepted in lot of 12 X 500 gm, 20 X 100 gm. Order for
liquid items like Inorganic Acids, Ammonia Solution, Organic Solvents, other chemicals are accepted in
lots of 20 X 500 ml (Plastic Bottles), 4 X 2.5 ltr and 2 X 5 ltr. In case of chemical in powder form, the order
size begins with 12 X 500gm. A similar standard is followed for Media supplements and other products.
This is essential to avoid breakage during transit. Every possible care is taken care during packaging as a
standard operating procedure. Though we cannot assure breakage/ leakage on delivery, we accept no
responsibility of transportation.
Highly inflammable and corrosive materials are not accepted by road carriers and passenger trains and
hence quick delivery cannot be assured. To know about material safety for product, Material Safety Data
Sheet (MSDS) is available on website www.tmmedia.in.
If requested, the goods can be insured and charged on the invoice.
SERVICES
ORDERS
PRICE
TAXES AND DUTIES
STANDARD PACK SIZE
DANGEROUS GOODS
INSURANCE

A/C Name
Bank Name
A/C No.
IFSC Code
Branch Add.
Titan Biotech Limited
HDFC Bank
B-34, Ashok Vihar, New Delhi-110052
HDFC 0001261
50200014683687
TM Media takes pride to provide the best customer service. As a mandate, the processing and
delivery time is followed within the specified schedule as shared on the sales quote. In case of orders
that require special handling, an additional fee might be applicable. All orders of the net value (after
discount) of Rs. 15,000/- or more will be supplied F.O.R destination by general road transporter (if the
location falls under ODA location supply would be till transporter allows). For orders with net value
less than Rs. 15,000/-, the consignment will be dispatched on freight to pay basis. This is not
applicable for the products on Page no.177-186. Freight charges will be prepaid and added to the
invoice as a separate item. There is a minimum charge per shipment for shipping and handling. A
hazardous charge will be processed for shipment of hazardous materials. We recommend
consolidating all hazardous materials onto a single order if possible to minimize hazardous charges.
Please report all questions regarding a shipment to Customer Service within 7 days of receipt.
Special prices will be provided on net basis both for Export and Bulk quantity enquiries.
Payment must be made along with orders. In case of credit of 30 days is given from the date of receipt
of the consignment, the terms of payment must be met without any delay. In case the party fails to
adhere, an interest of 24% will be charged from the due date. Payments must be made in Demand
Draft, Pay Order, Cheques drawn in favour of “Titan Biotech Ltd.”
DELIVERY & FREIGHT
EXPORT AND BULK QUANTITY ENQUIRY
PAYMENTS
Standing orders are welcome and such requests must be received at least 10 working days prior to
the scheduled shipment.
In the event of damaged goods, it is advised to retain goods and packaging. Kindly advise our office
and the local carrier without delay.
Any issue related to product return must be coordinated with our representative. Request for returns
must be made within 7 days of receipt. No returns will be entertained if there has been any error from
customer. Perishable or temperature sensitive products cannot be returned for any reason. These
items include but are not limited to:
The return will be accepted after due submission of adequate information, sufficing the reason of
return. If the requested information is not received within 7 days we shall consider that we have dealt
with complaint and the matter will be treated as “closed”. Full and final replacement along with return
invoice should be completed within 15 days.
No imitation of our brand logo or brand packaging is allowed. If found otherwise, TM Media has full
authority to take such situation in court for legal action. Titan Biotech Ltd will take no responsibility of
any damage caused by fake or imitated products. It is advised that the customers must avoid such
sellers for their own best interests. To know if the product is authentic, please reach out to
customercare@titanmedia.in
STANDING ORDERS
DAMAGE
RETURNS
COPYRIGHT
• Blood Culture Media • Reagents • Diagnostic Tests • Prepared Culture Media
• Susceptibility and Differentiation Disks • Dehydrated Culture Media • Chemicals
BANK DETAILS

All the product information and quality control specifications, which include ISO guidelines, cGMP
guidelines, USP/JP/EP/BP and also CLSI guidelines are provided in the TM Media Manual. For a copy
of the manual please contact your local Technical Sales Representative or Technical Service at
customercare@titanmedia.in. Also, for Technical Data (TD), Material Safety Data Sheet (MSDS),
Certificates of Analysis (COA) are available 24/7 on www.tmmedia.in.
All the items listed here are for Laboratory use only and we assume no responsibility whatsoever,
if used otherwise.
We and our authorized stockists shall not be responsible for any delay or part supply due to
circumstances beyond our control suct as riots, strikes, civil commotion, shortage of raw material,
natural calamities, government restrictions and budgetary changes by the Government etc.
However, the period of delay would be informed to the customers.
All disputes are subject to Alwar, Rajasthan jurisdiction only.
TECHNICAL SUPPORT AND DOCUMENTATION
DISCLAIMER
FORCE MAJURE
JURISDICTION
For
Enquiry
+91-011-71239900
www.tmmedia.in
marketing@titanbiotechltd.com

Peptone, Protein Hydrolysate, Infusions and Extracts
With pleasure, we introduce our updated Product list for the financial year 2018-19. As a commitment to
this industry, even this year we have introduced newer products. Titan Biotech Ltd. started its journey in
1992 to pursue innovation in the field of biotechnology and advanced life science. The strong hold on
technical expertise has given wings to our business journey with the remarkable market presence in more
than 77 countries. Under the brand 'TM Media', Titan Biotech Limited has flourished with its microbiology
products in full length around the world.
The Research and Development department supports our objective to evolve and keep pace with the
industry's growing needs.The product range has emerged manifold with more than 1800 products for
categories including Biological Media Bases, Dehydrated Culture Media, Media Supplements, Readymade
Culture Media (in all forms), Molecular Bio Grade Chemicals, Antibiotic Sensitivity Discs, Plant Tissue
Culture Media, Laboratory Chemicals and Microbiological Consumables. We understand our customers'
choices very well, thus Culture Media are available from veg and non veg sources. TM Media has
expatiated its product list for applications in various industries including Pharmaceutical, Nutraceutical,
Medical & Diagnostics, Food & Beverages, Biotechnology & Fermentation, Cosmetic, Veterinary & Animal
Feed, Agriculture industries as well as for research projects.
Our quality standards of production facility and products are regulated and certified by International
organizations. We are glad to have support from Pharmexcil, Capexil, FIEO and AIFPA for trade relations.
The entirety of the company is grounded with belief in relationship and quality. We own the responsibility
to fulfill customers' particular requirements. The formula has been simple for us that our progress is when
our customers progresses.
Suresh Singla
MANAGING DIRECTOR
The Brand-TM Media

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CERTIFICATES
* Will be updated soon.
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(Anil Mehta)
(Designated Officer)
Stamp and signature of Designated Officer
Central Licensing Authority under FSSA, 200605/09/2017
E-540, Industrial Area, Chopanki,
Bhiwadi, Alwar (Rajasthan), -301019
Manufacturer, Importer
18/05/2016
18/05/2015
17/05/2021
17/05/2016
Rs. 7500
(Modificaton)
Rs. 7500
Please refer to annexure.
Please refer to annexure.
Please refer
to annexure.
Please refer
to annexure.
* Will be updated soon.

QUALITY POLICY
We at Titan Biotech
Limited have committed
our state of the art technology
towards quality management and
quality assurance. The motto of the
company is to achieve customer
satisfaction with the desired quality
product at competitive cost. The company
is invested with well-trained professionals to
follow International Quality Standards. The
management assures the compliance with
periodic reviews and mystery audits.
Mandatorily, every production batch is
tested and its COA and MSDS are
recorded in the pre-defined format.
Suresh Singla
MANAGING DIRECTOR

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TM 341NUTRIENT AGAR
A general purpose medium used for cultivating a wide variety of microorganisms
Composition
Ingredients Gms/Ltr.
Agar
Peptone
Sodium Chloride
Beef Extract
Yeast Extract
15.00
5.00
5.00
1.50
1.50
* Dehydrated powder, hygroscopic in nature, store in a dry place in tightly- sealed
containers25°CandprotectedfromdirectSunlight.
Instructions foruse
Dissolve 28gms in 1000ml distilled water. Gently heat to boiling with gentle
swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi
(at121°C)for15 minutes.Coolto45- 500C priortouse.
Appearance: Lightcreamcolour, veryslightlyopalescentgel
pH(at25°C):7.4 ±0.2
Principle

1 2 3 4 5 6 7
Expected performance during specified best before period
when stored as directed.
2827
26
25
24
9
1021 202223
1415 13
8
19 18 16 12 11
customercare@tm media.in
17
TM 405
500gm
Expiry Date8.
Caution7.
For in Vitro Diagnostics5.
Received/Opened Date4.
Use of the Product3.
Synonym of Product Name2.
Product Name1.
ISO Certification Number 13485:20166.
ISO Certification 9001:2015 & 11133:201418.
Website URL17.
Customer Care Cell (Contact No. & Email ID)16.
Manufacturer Symbol15.
Symbol for European Conformity14.
Company Name & Address11.
Copyright Clause10.
Country of Origin9.
12. Company Logo
Symbol for Certification of
Current Good Manufacturing Practices
13.
Product Code Number20.
QR Code Scan for Technical Data19.
Composition of lthe Medium27.
pH Range26.
Instructions for Preparation of Medium25.
Disposal Procedure24.
Product's Brand Name23.
Lot/Batch Number22.
Pack Size21.
Formula Weight29.
Label
Packaging
Molecular Biology Grade Chemicals
5 Kg
1 Kg
500 gm
TM 1199
1 Kg
M1BJ9KR01
M1BJ9KR01
CHROMOGENIC UTI AGAR
for identification and confirmation of microorganisms
causing urinary tract infections
TM 1199
500 gm
M1BJ9KR01
M1BJ9KR01
CHROMOGENIC UTI AGAR
1 Kg500 gm100 gm
Sep
.202
1
NUTRIENT AGARgeneral purpose
medium
TM 1199
100 gm
M2CK9KR02
5 Kg 25 Kg

Index
92-94( )Biological Media Bases Including Veg.
89-91Media Supplements
1-77( )Dehydrated Culture Media Including Veg.
Chromogenic Dehydrated Culture Media ...............................................................
Dehydrated Culture Media (as per BIS) .....................................................................
Dehydrated Culture Media (as per ISO) .....................................................................
Harmonized Culture Media (Pharmaceuticals Testing) (as per USP/JP/EP/BP) .......................
Sterile Dehydrated Culture Media .................................................................
78-79
80-82
83-86
87
88( - irradiated)
95-101Ready-To- Use-Media
Ready-To-Use Media Plates .....................................................................................
Ready-To-Use Agar Media & Broth Media ....................................................................
Ready-To-Use Liquid Medium .......................................................................................
Ready-To-Use Kits & Slants ..........................................................................................
Blood Culturing System
Transport Swab with Medium
(Ready-To-Use Broth Media) ...........................................................
....................................................................................
Viral Transport Swab Kits ...........................................................................................
95-96
97
98
99
100
100
101
Anaerobic Box System, Anaerobic Jar System, Spreaders, Disposable Loops &
Inoculating Metal Loop Holder & Loops .................................................................... 102
Petri Plates 103................................................................................................................
Microbiological Lab Consumables 102-103
Infrared Sterilizer for Inoculation Loops .................................................................... 104
Microbial Air Monitoring Systems (Air Sampler) ................................................................. 104
Microbiological Lab Equipments 104

Index
105Differentiation Discs & Test Strips
106-108Antibiotic Sensitivity Discs (as per CLSI & Non CLSI)
109Staining and Indicator Solution & Readymade Staining Kits
110Analytical Reagent & Standard Solutions
111-112Biological Indicators & Chemical Indicators
113-115Plant Tissue Culture Media
116-123Plant Tissue Culture Media Ingredients
124-126Pure Antibiotic Powder
127-130Molecular Biology Grade Chemicals
131-175( )EP & AR GRADELaboratory Chemicals
175pH Indicator Paper Strips
176Acids & Solvents
179Food Additives
180-181Agro Products
182-184Biotechnology & Fermentation Products (Bacteriological Grade)
185Pharmaceuticals, Healthcare & Nutraceutical Products
186Veterinary & Animal Feed Ingredients
177-178Food Grade Chemicals

Product Specification
PRODUCT SUMMARY AND EXPLANATION :
Coliforms including Escherichia coli are used as primary indicators of faecal
contamination in water and food industries. Their presence and enumeration in samples
is used as an index of the presence of faecal matter and is indicative of the possible
presence of enteric pathogens. Chromogenic Coliform Agar (CCA) is a fast, accurate,
and efficient way to detect coliforms and E. coli during microbiological quality testing of
water and food samples. Escherichia coli are also the most common pathogen in urinary
tract infections. This product incorporates the company's chromogenic galactoside that
detects clinical levels of coliforms with high sensitivity.
TM 1858TM 1858 CHROMOGENIC COLIFORM AGAR (ISO 9308-1:2014)CHROMOGENIC COLIFORM AGAR (ISO 9308-1:2014)
INTENDED USE
For determination of coliform bacteria particularly Enterobacteriaceae on the basis of their ability to ferment lactose.
PRINCIPLE :
Chromogenic Coliform Agar (CCA) is used for enumeration of Escherichia coli and other coliforms in water samples by membrane filter
technique. This medium contains enzymatic digest of casein, yeast extract, sorbitol and sodium pyruvate as sources of carbon, nitrogen,
fermentable carbohydrate and other essential growth nutrients for the growth of microorganisms. Disodium hydrogen phosphate and
sodium dihydrogen phosphate are the buffering agents. Sodium chloride maintains the osmotic equilibrium in the medium. L- Tryptophan
improves the indole reaction and gives improved differentiation between Escherichia coli and other coliforms. Tergitol- 7 inhibits gram
positive bacteria. The two chromogens used; salmon-ß-D-galactoside and X-glucuronide help in differentiation of Escherichia coli and other
coliforms on the basis of colony colour. The enzyme ß-D-galactosidase cleaves salmon-ß-D-galactoside, and gives a salmon to red colour to
the coliform bacteria. E. coli have ß-D galactosidase and ß-D-glucuronidase enzymes to cleave both the chromogens, which give blue-violet
colour to colonies. Expression of ß-D-galactosidase is strengthened in the presence of IPTG. Agar is a gelling agent.
INSTRUCTION FOR USE :
1. Dissolve 26.45 gms in 1000 ml of distilled water.
2. Gently heat to boiling with gentle swirling, to dissolve the medium completely (Do not autoclave the medium and avoid overheating).
3. Cool to 45-50°C and pour into sterile petri plates.
QUALITY CONTROL SPECIFICATIONS :
Appearance of powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light yellow coloured opalescent gel forms in Petri plates.
pH (at 25°C) : 6.8 ± 0.2
Escherichia coli - Dark blue to violet colonies
Citrobacter freundii - Pink to red colonies
Salmonella enteritidis - Colourless colonies
Enterobacter aerogenes - Pink to red colonies
COMPOSITION
Agar
Sodium Chloride
Disodium hydrogen phosphate
Sodium dihydrogen phosphate (2H O)2
Yeast extract
Enzymatic digest of casein
10.00
Sodium pyruvate
Sorbitol
Tryptophan
Salmon-â-D-galactoside
Sodium heptadecyl sulphate (Tergitol 7)
X-glucuronide
5.00
2.70
2.20
2.00
1.00
1.00
1.00
1.00
0.20
0.15
0.10
Isopropyl 1-â-D-thiogalactopyranoside (IPTG) 0.10
Gms/Ltr.Ingredients
INTERPRETATION :
Cultural characteristics observed after incubation at 35 - 37°C for 18 – 24 hours.
ATCC
25922
8090
13048
13076
29212
Inoculum
(CFU/ml)
310
310
310
310
310
Appearance of Colony
Dark blue to violet colonie
Pink to red colonies
Pink to red colonies
Colourless colonies
-
Growth
Luxuriant
Luxuriant
Luxuriant
Luxuriant
Inhibited
Microorganisms
Escherichia coli
Citrobacter freundii
Enterobacter aerogenes
Salmonella enteritidis
Enterococcus faecalis
Recovery (%)
= 50%
= 50%
= 50%
= 50%
0%

PRINCIPLE :
Medium contains combination of Pancreatic digest of casein and papaic digest of
soyabean meal makes this media nutritious by providing amino acids and long chain
peptides for the growth of microorganisms. Sodium chloride maintains the osmotic
balance.Agar acts as solidifying agent.
The initial specimens should be inoculated onto another suitable medium and incubated for 18-24 hours in an aerobic atmosphere
supplemented with carbon dioxide. Many pathogens require carbon dioxide on primary isolation therefore plates may be incubated in an
atmosphere containing approximately 3 - 10% CO at 35 ± 2ºC for 24 - 48 hours and on maximum incubation period for 3 – 5 days at
30ºC – 35º C by using membrane filtration, spread plate and pour plate method. Choose one or two well-isolated colonies those resemble
Haemophilus species and perform Gram-staining to confirm that the isolate is a gram-negative rod or cocco - bacillus. Prepare suspension of
10²CFU/ml (10-15 colonies) in 5 ml sterile, purified water or Soya casein digest broth and vortex to mix. Dip a swab in the suspension and
inoculate the entire surface of the plate with the swab. With sterile forceps, place a Taxo X factor strip, a V factor strip and a XV strip on the
plate, at least 20 mm apart. These strips are impregnated with Haemoglobin, routinely used for differentiation and isolation of Haemophillus
sp. After incubation, isolated colonies of organisms from the original sample have been obtained such as Staphylococcus aureus. The
colonies are sub cultured by interest so that the positive identification can be made by means of biochemical and/or serological testing.
Soya Casein Digest Agar is general purpose medium used with or without blood for
enrichment and isolation of fastidious microorganisms. It is a good medium for
isolation of anaerobes. This medium is used for a multitude of purposes including
maintenance of stock cultures, plate counting, and isolation of microorganisms from a
variety of specimen types and as a base for media containing blood.
TM 345TM 345 SOYA CASEIN DIGEST AGAR (as per USP)SOYA CASEIN DIGEST AGAR (as per USP)
INTENDED USE
For enrichment and isolation of fastidious microorganisms with or without blood.
Gms/Ltr.Ingredients
COMPOSITION
Pancreatic digest of casein
Agar
Soyatone (Soya Peptone)
Sodium chloride
15.00
15.00
5.00
5.00
INSTRUCTION FOR USE
1. Dissolve 40g in 1000ml distilled water.
2. Gently heat to boil with gentle swirling and dissolve the medium completely.
3. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
4. Cool the medium at 45-50ºC.
5. Add 5% defibrinated sheep blood if required.
6. Gently shake to avoid bubbles and pour into sterile Petri plates as desired.
:
Appearance of Powder : Cream to yellow colour, homogenous mixture, free flowing powder
Appearance of prepared medium :
Basal medium : Light yellow coloured clear to slightly opalescent gel.
After addition of 5-7%w/v sterile defibrinated blood : Cherry red coloured opaque gel
pH (at 25°C) : 7.3 ± 0.2
CULTURAL RESPONSE :
3
Cultural characteristics observed after inoculating (10 CFU/ml) with added 5% defibrinated sheep blood or without blood, after
inoculation and incubation as mentioned.
Staphylococcus aureus
Continued

*Anaerobic incubation, NA; not applicable
Incubation at 30 - 35°C for 18 - 24 hours
Incubation at 30 - 35°C for 18 - 48 hours
Incubation at 20-25°C up to 5 days
Recovery (%)
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
= 70%
w/added blood
Beta
Beta
NA
NA
NA
NA
NA
NA
NA
NA
Alpha
NA
NA
NA
NA
ATCC
6538P
25923
8739
25922
9027
27853
6633
14028
13813
29212
6305
9341
19404
10231
16404
Inoculum
(CFU/ml)
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
Microorganisms
Streptococcus pneumoniae
Pseudomonas aeruginosa
Salmonella typhimurium
*Clostridium sporogenes
Escherichia coli
Escherichia coli
Bacillus subtilis
Klebsiella pneumoniae
Micrococcus luteus
Candida albicans
Aspergillus brasiliensis
Continued

PRINCIPLE :
Medium contains dextrose in high concentration which is used as an energy source. Mycological Peptone provides nitrogen and vitamin
source required for the growth of the organism and Agar works as a solidifying agent. The medium has an acidic pH (5.6) provided by
Mycological peptone which promotes the growth, formation of Sporangia and condia as well as the formation of yeasts and molds. High
dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from test samples. Due to its low pH this
medium is very sensitive to overheating which will soften the agar and caramelize the carbohydrate. Chloramphenicol supplement or
antibiotics if added to the medium can increase the sensitivity of the medium and inhibit bacterial growth.
40.00
15.00
10.00
Dextrose
Agar
Mycological peptone
COMPOSITION
TM 387TM 387 SABOURAUD DEXTROSE AGARSABOURAUD DEXTROSE AGAR
Sabouraud Dextrose Agar is a modified Dextrose Agar employed for the isolation of
saprophytic and pathogenic fungi from sources containing large number of fungi and
bacteria. The medium is also used cultivation of yeasts, moulds and aciduric
microorganisms from food, contamination in cosmetics and clinically to aid in
diagnostics of yeast and fungal infections. Characteristics features of fungi and molds,
such as sporing structures and pigmentation are well developed on this medium.
1. Dissolve 65 gms in 1000 ml distilled water.
2. Gently heat to boiling with gentle swirling and dissolve the medium is completely.
3. Sterilize by autoclaving at 15psi (121°C) for 15 minutes.
4. Cool to 45 – 50°C prior to dispense into sterile Petri - plates.
INTENDED USE
For cultivation of yeasts, molds and aciduric microorganisms.
Appearance of Powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light amber colour, clear to slightly opalescent gel
pH (at 25°C) : 5.6 ± 0.2
Candida Albicans
CULTURAL RESPONSE :
Cultural characteristics observed after incubation at 25-30°C for 4-6 days for fungi and for bacteria at 33-3 .5°C for 24-48 hours
Aspergillus brasiliensis
Candida albicans
Penicillum corylophilum
Saccharomyces cerevisiae
Trichophyton mentagrophytes
Lactobacillus casei
Microorganisms
Zone diameter
≥ 70%
Zone diameter
Zone diameter
≥ 70%
≥ 70%
Recovery rate (%)
Point inoculation
Point inoculation
Point inoculation
54
10 - 10
54
10 - 10
54
10 - 10
Inoculum (CFU/ml) Growth
Good
Good
Good
Good
Good
Good
ATCC
16404
10231
20203
9763
9533
9595

TM 339TM 339 MUELLER HINTON AGARMUELLER HINTON AGAR
PRINCIPLE :
The medium consist of Beef extract and Casein acid hydrolysate which provides nitrogen, vitamins, carbon, and amino acids. Starch is added
to absorb any toxic metabolites produced. Agar is the solidifying agent. The thymine/thymidine content of this medium is minimized
(determined by disc diffusion procedure with Enterococcus faecalis ATCC 29212 and sulfamethoxazole-trimethoprim antibiotic) and levels of
calcium and magnesium are adjusted (determined by Pseudomonas aeruginosa ATCC 27853 and aminoglycoside antibiotics) to give
consistent zones of inhibition as per specified diameters in the CLSI standards. For fastidious organisms, Mueller–Hinton fastidious (MH-F)
agar is prepared by supplementing the basal media with 5% (v/v) mechanically defibrinated horse blood and 20 mg/liter ß-NAD. The
standardized disc diffusion procedure is based on the ability of an antimicrobial agent impregnated on a paper disc to diffuse through agar gel.
Mueller Hinton Agar is used for cultivation of Neisseria & for
determination of susceptibility of microorganisms to antibiotics. It is
formulated by Mueller and Hinton for the primary isolation of Neisseria
species. Bauer and Kirby recommended this medium for performing
antibiotic susceptibility tests using a single disc of high concentration. It
has become the standard medium for antimicrobial susceptibility testing
and its performance is in accordance to Clinical and Laboratory Standard
Institute (CLSI), formerly NCCLS and complies with requirements of the
WHO, FDA and EUCAST.
COMPOSITION
*Beef, infusion form
Casein acid hydrolysate
Agar
Starch
300.00
17.50
17.00
1.50
[*300gm of infusion from beef is equivalent to 2.0gm of beef extract]
2. Gently heat to boiling with gentle swirling and dissolve the medium completely.
3. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. DO NOT OVERHEAT.
4. Cool at 45 - 50°C and pour into sterile Petri plates to achieve an even depth of 4.0 ± 0.5 mm.
Note: For fastidious organisms, Mueller–Hinton fastidious (MH-F) agar is prepared by supplementing the basal media with 5% (v/v)
mechanically defibrinated horse blood and 20 mg/liter â-NAD. Prepare the media according to instruction and add supplements after cooling to
42–45°C. Mix well and pour into sterile Petri plates to achieve an even depth of 4.0 ± 0.5 mm.
INTENDED USE
For cultivation of Neisseria & for determination of susceptibility of microorganisms to antibiotics.
Appearance of Powder : Cream to yellow colour, homogeneous mixture, free flowing powder.
Appearance of prepared medium :
Basal medium : Light to medium amber colour, clear to slightly opalescent gel
After addition of blood : Bright red colour, opaque gel
pH (at 25°C) : 7.3 ± 0.2
Antibiotic susceptibility testing
Autoclave and cool the medium to 45-50°C. Pour into sterile Petri plates. The agar must be 4 ± 0.5 mm thick. Let solidify on a cold surface. Dry
the plates in an incubator with the covers partially removed in order to avoid the formation of water droplets on the surface of the agar, a
phenomenon, which can deteriorate the diffusion qualities of the medium. Prepare, inoculate and dispense antibiotic discs following the
procedure described by CLSI. Press the disk containing the antimicrobial on the agar surface. Incubate at 35 ± 1°C for 16-20 hours and then
measure the inhibition zone with a compass and scale.
Escherichia coli
GEN
10
TOB
10
Staphylococcus aureus Pseudomonas aeruginosa
TOB
10
GEN
10
TOB
10
GEN
10
Continued

Growth promotion test
Cultural characteristics observed after incubation at 35 ± 1°C for 16 – 20 hours.
Inoculum (CFU/ml) Growth
Luxuriant
Luxuriant
Luxuriant
Luxuriant
3
10
ATCC
25923
29212
25922
27853
3
10
3
10
3
10
Test strains
Escherichia coli
Antibiotic susceptibility test
Cultural characteristics observed after inoculating 0.5 McFarland culture (1-2 x 108 CFU/ml) by lawn technique, dispensing antibiotic discs
and incubation at 35 ± 1°C for 16 - 20 hours. After incubation, inhibition zone diameter measured in mm.
Microbiological parameters
Continued
(ATCC 27853)
Std
Escherichia coli
(ATCC 25922)
Std
(ATCC 25923)
StdAntibiotics
Amikacin (30 mcg)
Amoxicillin (10 mcg)
Ampicillin (10 mcg)
Azithromycin (15 mcg)
Colistin(Methane Sulphate (10 mcg)
Cefaclor (30 mcg)
Cefoperazone (75 mcg)
Cefazolin (30 mcg)
Cefuroxime (30 mcg)
Cephalothin (30 mcg)
Chloramphenicol (30 mcg)
Ciprofloxacin (5 mcg)
Erythromycin (15 mcg)
Gentamicin (10 mcg)
Kanamycin (30 mcg)
Norfloxacin (10 mcg)
Lomefloxacin (10 mcg)
Aztreonam (30 mcg)
Cefepime (30 mcg)
Linezolid (30 mcg)
*Incubation at 35 ± 1°C for 16 - 20 hours, in air with 4-6% CO2 on MH-F agar.
19 - 25
15 - 22
19 - 26
Resistant
11 - 17
23 - 27
28 - 34
21 - 27
20 - 26
15 - 21
21 - 27
30 - 40
Resistant
19 - 26
14 - 17
28 - 35
27 - 33
28 - 36
31 - 37
Resistant
28 - 36
27 - 35
20 - 26
21 - 26
Resistant
27 - 31
24 - 33
29 - 35
27 - 35
29 - 37
19 - 26
22 - 30
22 - 30
19 - 27
19 - 26
17 - 28
23 - 29
Resistant
23 - 29
25 - 32
Resistant
Resistant
18 - 26
Resistant
11 - 17
Resistant
23 - 29
Resistant
Resistant
Resistant
Resistant
25 - 33
Resistant
17 - 23
Resistant
22 - 29
22 - 28
23 - 29
25 - 31
Resistant

1. Dissolve 89.0 in 1000 ml distilled water.
2. Gently heat to boil with gentle swirling and dissolve the medium completely. DO NOT AUTOCLAVE.
3. Cool to 45 - 50°C and pour into sterile Petri plates.
gms
Sucrose
Agar
Ox bile
Proteose peptone
Yeast extract
Sodium citrate
Ferric citrate
Sodium thiosulphate
Bromothymol blue
Sodium chloride
Thymol blue
Ingredients
COMPOSITION
Gms/Ltr.
20.00
15.00
10.00
10.00
10.00
10.00
8.00
5.00
1.00
0.04
0.04
TM 436TM 436 TCBS AGAR (VIBRIO SELECTIVE AGAR)TCBS AGAR (VIBRIO SELECTIVE AGAR)
PRINCIPLE :
Proteose peptone and Yeast extract are the sole sources of carbon, nitrogen, vitamin B - complex, minerals and amino acids in the medium. Sodium
thiosulphate serves as a sulphur source and, in combination with Ferric citrate, detects hydrogen sulphide production. Sucrose is included as a
fermentable carbohydrate for the metabolism of Vibrio. Inhibition of gram- positive bacteria and suppression of coliforms is achieved by the
incorporation of Ox bile, which is a synthetic compound and suppresses primarily Enterococci. Thymol blue and Bromothymol blue are included as
indicators of pH changes. Sodium citrate and Sodium thiosulphate are the selective agents, providing an alkaline pH and improves the recovery of
V.cholerae. Strains of V.cholerae produce yellow colonies on TCBS Agar because of fermentation of sucrose. V.alginolyticus also produce yellow
colonies. V.parahaemolyticus is a sucrose non-fermenting organism and therefore produces blue-green colonies, as does V.vulnificus. and inhibit
gram- positive organism and suppress coliforms. Agar is the solidifying agent.
INTENDED USE
For selective isolation of Vibrio cholerae & other enteropathogenic Vibrios causing food poisoning.
Appearance of Powder : Light yellow to light tan colour, homogeneous mixtire, free flowing powder
Appearance of prepared medium : Bluish green colour, clear to slightly opalescent gel.
pH (at 25°C) : 8.6 ± 0.2
Vibrio cholera
Vibrio vulnificus
Vibrio fluvialis
Shigella flexneri
Vibrio parahemolyticus
Escherichia coli
Proteus vulgaris
Microorganisms
15748
29307
33809
17802
12022
29212
25922
13315
ATCC
10³
10³
10³
10³
>=10³
>=10³
>=10³
>=10³
Inoculum
(CFU/ml)
= 50%
= 30%
= 50%
0%
= 50%
0%
0%
0%
Recovery (%)
Yellow
Greenish yellow
Yellow
----
Bluish green
----
----
----
Good-Luxuriant
Growth
Good-Luxuriant
Good-Luxuriant
Fair-Good
Inhibited
Inhibited
Inhibited
Inhibited
CULTURAL RESPONSE
Cultural characteristics observed after incubation at 35-37°C for 18 – 24 hours.
Vibrio sp.
(Yellow colonies)
TCBS Agar (Vibrio Selective Agar) is highly selective for the isolation of Vibrio cholerae and
V. parahaemolyticus from stool specimens, and specified in standard methods for food
testing. This highly selective medium meets the nutritional requirements of Vibrio species
and allows competing with intestinal flora. Vibrio species is able to grow in media containing
increased salt concentrations. Vibrio species are natural inhabitants of water.

PRINCIPLE :
This medium consists of Beef extract and Peptic digest of animal tissue provide nitrogen, vitamins, minerals and amino acids essential for
growth. Lactose is the fermentable carbohydrate providing carbon and energy. Bile salts and Sodium citrate inhibit Gram-positive bacteria,
most coliform bacteria and swarming Proteus spp., while allowing Salmonella spp to grow. Brilliant green and high concentrations of Sodium
thiosulphate and citrate largely inhibit the accompanying microbial flora. Sulphide production is detected by using thiosulphate and iron ions,
the colonies turn black. The presence of coliform bacteria is established by detecting degradation of lactose to acid with the pH indicator
neutral red. Neutral red is the pH indicator. Non-lactose fermenting bacteria (supposed pathogens) produce clear colonies, transparent or
colorless, while coliforms are sufficiently inhibited, and form small colonies that vary from pink to red in color. The plates of the medium can
be kept for at least a week in refrigeration. This formulation, highly selective, is not recommended for the primary isolation of Shigella. Some
Shigella spp. may be inhibited.
2. Gently heat to boiling with gentle swirling and dissolve the medium completely. DO NOT AUTOCLAVE.
3. Cool to 45 – 50°C and distribute into sterile petri plates.
TM 386TM 386 SS AGAR (SALMONELLA SHIGELLA AGAR)SS AGAR (SALMONELLA SHIGELLA AGAR)
SS Agar was originally developed as a selective medium for the isolation of Salmonella
and Shigella species. It was also developed to aid in the differentiation of lactose and
non-lactose-fermenters from clinical specimens, suspected foods, and other such
samples. SS Agar is a moderately selective medium in which gram-positive bacteria are
inhibited by bile salts, brilliant green and sodium citrate.
For differential and selective isolation of Salmonella and Shigella species from pathological samples and foods.
INTENDED USE
Appearance of Powder : Light yellow to pink colour, homogeneous mixture, free flowing powder
Appearance of prepared medium : Reddish orange in colour, clear to slightly opalescent gel
pH (at 25°C) : 7.0 ± 0.2
5
10
3
10
3
10
3
10
3
10
5
10
Inoculum
(CFU/ml)
Microorganisms
Escherichia coli
Proteus mirabilis
Shigella flexneri
Salmonella typhi
ATCC
25922
29212
25933
12022
6539
14028
Growth
Fair
Poor
Fair
Good
Luxuriant
Luxuriant
Recovery on
test media (%)
20-30 %
= 10%
30 - 40%
40 - 50%
= 50%
= 50%
Colourless colonies
may have black centers
Colourless colonies
Colourless with black centers
Colourless with black centers
Pink colonies with bile ppt.
Colourless colonies
CULTURAL RESPONSE :
Cultural characteristics observed after incubation at 35 ± 2°C for 18 - 24 hours.
15.00
10.00
10.00
8.50
8.50
5.00
5.00
1.00
0.025
0.00033
Agar
Sodium citrate
Lactose
Bile salts
Sodium thiosulphate
Ingredients
COMPOSITION
Gms/Ltr.
Beef Extract
Peptic digest of animal tissue
Ferric citrate
Neutral red
Brilliant green

Control, S. Typhi & S. Typhimurium
PRINCIPLE :
The media contains Peptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance and
phosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and
supports intense growth. The phosphate buffer system prevents bacterial damage due to changes in the pH of the medium.
Appearance of Powder : Cream to yellow colour, homogeneous mixture, free flowing powder
Appearance of prepared medium : Light yellow colour, clear solution without any precipitate
pH (at 25°C) : 7.2 ± 0.2
Buffered Peptone Water is a non-selective pre-enrichment medium for the isolation for the
Salmonella species from food and associated samples. The medium is designed to be used prior
to selective enrichment. As Salmonella may be present in low number or sub-lethally injured
pre-enrichment allows cells time to repair and multiply before being introduced to selective
culture, thereby improving the chances of recovery from sample.The composition and
performance criteria of this medium are as per the applications laid down in ISO 6579-2002,
whereas the quality control of Buffered peptone water includes testing in accordance with ISO
6579:2017 and ISO 11133-2014.
TM 307TM 307 BUFFERED PEPTONE WATER (ISO 6579-1:2017/11133:2014)BUFFERED PEPTONE WATER (ISO 6579-1:2017/11133:2014)
INTENDED USE
For pre-enrichment of injured Salmonella species prior to selective enrichment and isolation.
1. Dissolve 20.00 gms in 1000 ml distilled water.
2. Gently heat to boiling with gentle swirling and dissolve the medium completely.
3. Dispense 50 ml amount into each flask.
5. Cool at room temperature prior to use.
CULTURAL RESPONSE :
Cultural characteristics observed after incubation at 35 ± 2°C for 18 - 24 hours.
Salmonella enteritidis
Salmonella typhi
Microorganisms
Escherichia coli
13076
6539
14028
ATCC
25922
Inoculum
(CFU/ml)
3
10
3
10
3
10
3
10
Luxuriant
Growth
Luxuriant
Luxuriant
Fair - Good 30- 40%
= 50%
= 50%
= 50%
Recovery* (%)
*Recovery is observed on XLD Agar (TM 492)
COMPOSITION
10.00
5.00
3.50
1.50
Peptone
Sodium chloride
Sodium phosphate dibasic
Potassium phosphate monobasic
Gms/Ltr.Ingredients

PRINCIPLE :
Medium contain Peptone and Yeast extract are the source of nitrogen, sulphur, carbon, vitamins and minerals. Bile salt mixture and Crystal
violet are the inhibitors of gram-positive microorganisms. Lactose is the fermentable carbohydrate. Neutral red indicator, changes to red-
purple due to the formation of acid during fermentation which change the pH. Sodium chloride is for the osmotic balance. Agar is a solidifying
agent.
CULTURAL RESPONSE :
Cultural characteristics observed after incubation at 35ºC-37ºC for 18 – 24 hours
Escherichia coli
Enterobacter aerogenes
Salmonella Enteritidis
Microorganisms
25922
13048
13076
25923
ATCC
3
10
3
10
3
10
3
10
Inoculum
(CFU/ml)
>=50%
>=50%
>50%
0%
Recovery
rate (%)
Luxuriant
Luxuriant
Inhibited
Luxuriant
Growth Appearance of colony
Pinkish red with bile ppt.
Pink to pinkish red
Colourless to orangish Yellow
Violet Red Bile Agar, a modification of MacConkeys original formulation is used for the enumeration of coli-aerogenes bacterial group. It
relies on the use of the selective inhibitory components crystals violet and bile salts and the indicator system lactose, and neutral red. Thus,
the growth of many unwanted organisms is suppressed, while tentative identification of sought bacteria can be made. Organisms, which
rapidly attack lactose, produce purple colonies surrounded by purple halos. Non-fermenters or late lactose-fermenters produce pale
colonies with greenish zones. VRBA is recommended by APHA.
TM 426TM 426 VIOLET RED BILE AGARVIOLET RED BILE AGAR
INTENDED USE
For isolation and enumeration of coli-aerogenes in water, milk and other dairy food products.
COMPOSITION
Ingredients
Agar
Sodium chloride
Lactose
Yeast extract
Peptone
Bile salt mixture
Neutral red
Crystal violet
Gms/Ltr.
0.03
15.00
10.00
7.00
5.00
3.00
1.50
0.002
2. Gently heat to boiling with gentle swirling and dissolve the medium completely. DO NOT AUTOCLAVE.
3. Cool to 45ºC and pour into sterile petri plates.
Appearance of prepared medium : Reddish purple coloured clear to slightly opalescent gel
pH (at 25°C) : 7.4 ± 0.2
E.coli

PRINCIPLE :
Peptone special provides nitrogenous, carbonaceous compounds and other essential
growth nutrients while agar as a solidifying agent. UTI Agar contains two specific
Chromogenic substrates which are cleaved by enzymes produced by Enterococcus spp.,
Escherichia coli and coliforms. In addition, it contains phenylalanine and tryptophan,
which provide an indication of tryptophan deaminase activity, indicating the presence of Proteus spp.,Morganella spp.and Providencia spp. One of
the Chromogenic substrate is cleaved by ß- glucosidase possessed by Enterococci resulting in formation of blue colonies. E. coli produces pink
colonies due to the enzyme ß-D-galactosidase that cleaves the other Chromogenic substrate. Further confirmation of E. coli can be done by
performing the Indole test. Coliforms produce purple coloured colonies due to cleavage of both the Chromogenic substrate. Colonies of Proteus spp.,
Morganella spp. and Providencia spp. appear brown because of tryptophan deaminase activity.
Urinary tract infections are bacterial infections affecting parts of urinary tract. The
common symptoms of urinary tract infection are urgency and frequency of micturition,
with associated discomfort or pain. The common condition is cystitis, due to infection of
the bladder with a uropathogenic bacterium, which most frequently is Escherichia coli,
but sometimes Staphylococcus saprophyticus or especially in hospital-acquired
infections, Klebsiella species, Proteus mirabilis, other coliforms, Pseudomonas
aeruginosa or Enterococcus faecalis. Chromogenic UTI Agar is formulated on basis of
work carried out by Pezzlo Wilkie et al, Friedman et al, Murray et al, Soriano and Ponte
and Merlino et al.
TM 1199TM 1199 CHROMOGENIC UTI AGARCHROMOGENIC UTI AGAR
INTENDED USE
For identification and confirmation of microorganisms causing urinary tract infections.
1. Dissolve 32.45 in 1000 ml of distilled water.
2. Gently heat to boiling with gentle swirling, to dissolve the medium completely.
4. Cool to 45-50°C and pour into sterile petri plates.
gms
Appearance of Powder : Cream to yellow colour, homogeneous mixture, free flowing powder.
Appearance of prepared medium : Light amber colour, clear to slightly opalescent gel
pH (at 25°C) : 6.8 ± 0.2
COMPOSITION
Ingredients
Agar
Gms/Ltr.
15.00
15.00
2.450
Peptone, special
Chromogenic mixture
Escherichia coli - Pink- Purple
Enterococcus faecalis – Blue
Staphylococcus aureus - Cream yellow
Pseudomonas aeruginosa - Colourless
(greenish pigment may be observed)
Klebsiella pneumoniae - Bluish Purple
CULTURAL RESPONSE :
Cultural characteristics observed after incubation at 35 – 37 °C for 18- 24 hours
Escherichia coli
Klebsiella pneumoniae
Microorganisms
Proteus mirabilis
>=70%
>=70%
>=70%
>=70%
Recovery
rate (%)
>=70%
>=70%
Luxuriant
Luxuriant
Luxuriant
Luxuriant
Growth
Luxuriant
Luxuriant
25922
13883
25923
27853
ATCC
12453
29212
3
10
3
10
3
10
3
10
Inoculum
(CFU/ml)
3
10
3
10
Appearance of colony
Pink – purple
Bluish – purple
Cream – yellow
Colourless
(greenish pigment may be observed)
Light brown
Small blue

Salmonella Typhimurium
2. Gently heat to boil with gentle swirling and dissolve the medium completely. (DO NOT AUTOCLAVE. DO NOT RE-HEAT).
3. Cool to 45 - 50°C prior to dispense into sterile petri plates
PRINCIPLE :
The medium contains Yeast extracts as source of vitamins and minerals. Addition of Sodium deoxycholate acts as a selective agent which is inhibitory
to Gram-positive bacteria. It suppresses the growth of other enteric pathogens and enhances the growth of only few enteric bacilli. The medium
contains Xylose as a fermentable carbohydrate which is utilized by Salmonella species. The medium pH is changed due to fermentation of Xylose
which is detected by indicator Phenol red, thus the colony colour turns red. The medium also contains Lactose and Sucrose as the source of
fermentable sugar. L-lysine is an essential amino acid source. Lysine is added to differentiate Salmonella Sp. Sodium chloride helps maintaining the
osmotic balance of the cells. This medium also allows differentiation of bacilli based on their ability to produce H S; it contains Sodium thiosulphate2
and Ferric ammonium citrate that helps visualizing the black centered colonies on production of hydrogen sulphide in the medium. Agar is added as
the solidifying agent.
XLD Agar was formulated by Taylor for the isolation and differentiation of enteric pathogens including Salmonella Typhi from other Salmonella
species. XLD Agar has been recommended for the identification of Enterobacteriaceae and for the microbiological testing of foods, water and dairy
products. The media formulation does not allow the over growth of other organisms over Salmonella andShigella.
COMPOSITION
15.00
6.80
3.50
7.50
5.00
3.00
7.50
5.00
2.50
0.80
0.08
Agar
Sodium thiosulphate
Xylose
Sucrose
L-Lysine
Yeast extract
Lactose
Sodium chloride
Sodium deoxycholate
Ferric ammonium citrate
Phenol red
TM 492TM 492 XLD AGAR (XYLOSE LYSINE DEOXYCHOLATE AGAR) (ISO 6579:2002)XLD AGAR (XYLOSE LYSINE DEOXYCHOLATE AGAR) (ISO 6579:2002)
INTENDED USE
For selective isolation and enumeration of Salmonella typhi and other Salmonella species.
Appearance of prepared medium : Red coloured clear to slightly opalescent gel
pH (at 25°C) : 7.4 ± 0.2
Cultural Response
Cultural response was observed after incubation at 35-37°C for specified time. Recovery rate is considered as 100% for bacteria growth on Soyabean
Casein Digest Agar.
Proteus Vulgaris
Salmonella Paratyphi A
Salmonella Enteritidis
Salmonella Paratyphi B
Salmonella Typhi
Shigella Dysenteriae
Shigella sonnei
13315
9150
13076
8759
6539
13313
25931
6538
14028
29212 3
10
Good
Good
Good
Good
Good
Good
Fair to Good
Inhibited
Good
Inhibited
= 50 %
= 50 %
= 50 %
= 50 %
= 50 %
= 50 %
30-40 %
0%
= 50 %
0%
Red with black centres
Grey with black centres
Red
Red
-
-
Red
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
3
10
Microorganisms ATCC Inoculum (CFU/ml) Growth Recovery rate (%) Appearance of colony

This medium is both differential and enriched medium and used for the isolation,
cultivation and detection of hemolytic activity of fastidious microorganisms like
Streptococci, and Pneumococci. The Blood Agar plate medium contains highly
nutritious extract and the 5 % blood supplement that provides additional use in
isolation, cultivation, and determination of hemolytic reactions of fastidious
pathogenic microorganisms. Hemolytic patterns may vary with the source of animal blood or type of base medium used. In sheep blood,
nucleotidase destroys V factors preventing the growth of Hemophilus species on sheep blood agar unless other microorganisms, such as
Staphylococci, provide the V factors. Small amounts of reducing sugars inhibit the expression of ß-hemolytic, and ß-hemolytic Streptococci may
develop a green zone or ring of hemolysis.
A number of streptococcal species produce substances that lyses of the red cell wall releasing of hemoglobin. Such substances are referred to as
hemolysins. The activity of streptococcal hemolysins, streptolysins, can be readily observed when the organisms are growing on a blood agar
plate. Different streptococci produce different effects on the red blood cells in blood agar.
lThose that produce incomplete hemolysis and only partial destruction of the cells around colonies are called alpha-hemolytic
Streptococci. Characteristically, this type of hemolysis is seen as a distinct greening of the agar in the hemolytic zone, and thus this group of
streptococci has also been referred to as the viridans group.
lSpecies whose hemolysins cause complete destruction of red cells in the agar zones surrounding their colonies are said to be beta-
hemolytic which are small opaque or semi translucent colonies surrounded by clear zones in a red opaque medium. Two types of beta lysins
are produced; Streptolysin-O, an antigenic oxygen-labile enzyme and Streptolysin-S, a non-antigenic oxygen-stable enzyme. The hemolytic
reaction is enhanced when blood agar plates are streaked and simultaneously stabbed to show subsurface hemolysis by Streptolysin-O in an
environment with reduced oxygen tension. Some strains of Staphylococci, Escherichia coli, and other bacteria also may show beta-
hemolysis.
lSome species of Streptococci do not produce hemolysins. Therefore, when their colonies grow on blood agar, no change is seen in the red
blood cells around them. These species are referred to as nonhemolytic or gamma hemolytic streptococci.
COMPOSITION
14.00
5.00
12.50
4.50
5.00
4.50
Casein enzymic hydrolysate
Sheep Blood
Agar
Peptic digest of animal tissue
Sodium chloride
Yeast extract
TMP 017TMP 017 SHEEP BLOOD AGAR PLATESHEEP BLOOD AGAR PLATE
For cultivation of fastidious organisms and studying haemolytic reactions
INTENDED USE
(Showing â Haemolysis)
Either streak, inoculate or surface spread the test inoculum aseptically on the plate.
PRINCIPLE :
Medium contains nutritional components like pancreatic digest of casein, neutralized peptone, and yeast extract, and the addition of sodium
chloride provides an osmotically balanced medium for bacterial cells. The addition of 5% defibrinated sheep blood allows for the determination of
hemolytic reactions, an important differential characteristic.
Appearance : Cherry red coloured opaque gel forms in Petri plates.
Quantity of Medium : 25 ml of medium in 90 mm plates.
Streptococcus pneumoniae
Streptococcus pyogenes
Escherichia coli
Salmonella typhi
Organism
6303
19615
29212
25923
25922
6539
ATCC
3
10
3
10
3
10
3
10
3
10
3
10
Inoculum (CFU/ml)
luxuriant
luxuriant
luxuriant
luxuriant
luxuriant
luxuriant
Growth
>=70%
>=70%
>=70%
>=70%
>=70%
>=70%
Recovery rate (%)
beta
alpha
beta
beta
none
none
Colour of colony
INTERPRETATION :
Cultural characteristics observed after incubation at 30-35 °C for 18-48 hours.

Dehydrated Culture Medium is a combination of complex nutrient substrates formulated for the
cultivation of microorganisms. The components of a Dehydrated Culture Medium must satisfy the
nutritional requirements like nitrogen, carbon and trace elements of microorganisms, in order to live and
replicate.
The Dehydrated Culture media is meant for carrying out bacteriological work in the laboratory and is not
used for human or animal consumption directly or indirectly.
To cultivate bacteria, a suitable environment is must. The environment can either be in a solid media
form, known as Agar or a liquid form known as Broth. Broth media can be used in case of a known
pure culture. However, Agar medium can be used to isolate and enumerate bacterial populations.
There are several types of media which is used for laboratory cultures:
1. General Purpose Media: This is a non-selective and allows the growth of almost all aerobic and
facultative anaerobic organisms. It is used primarily for isolation of organisms. The most famous
example of general purpose media is Nutrient Agar (TM 341)
2. Enriched Media: This type of media contains specific nutrients, which supports the growth of
fastidious organisms. Blood Agar (TM 071) is an enriched medium in which nutritionally rich,
whole blood supplements is added and act as basic nutrients.
3. Selective Media: This media contains additives that enhance the growth of certain organisms
while inhibiting the growth of others. Selective media can be either moderately or highly selective.
Moderately selective media will help decrease the interference of unwanted bacteria, while any
highly selective media will completely eliminate undesired growth. Eosin Methylene Blue or EMB
(TM 336) that contains methylene blue which is toxic to Gram-positive bacteria and supports the
growth of Gram negative bacteria.
4. Differential Media: This media distinguish one microorganism type from another growing on
the same media. This type of media uses the biochemical characteristics of a microorganism
growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin,
or methylene blue) added to the medium to visibly indicate the defining characteristics of a
microorganism. MacConkey Agar is differential for lactose fermentation.
TYPES OF CULTURE MEDIA
CONSTITUENTS SOURCE
Blood Serum, Yeast Extract or Vitamins, Nicotinamide Adenine Dinucleotide
Phosphates, Acetates and Citrates
Chemicals, Antimicrobials and Dyes
Phenol Red, Neutral Red
Agar, Gelatine, Alginate, Silica Gel, Gellan Gum
Phosphate, Sulfate, Magnesium, Calcium, Iron
Amino-Nitrogen
Energy Sources
Buffer Salts
Selective Agents
Indicator Dyes
Gelling Agents
Mineral Salts and Metal
GENERAL INFORMATION
DEHYDRATED CULTURE MEDIA

5
10
15
25
30
20
PRESSURE
(in Pounds) o
C
108
116
127
131
121
TEMPERATURE
134
226
240
260
267
250
275
o
F
Note : Efficiency of the autoclave should be ascertained from time to time using various biological or chemical
indicators (Please check page 111-112 for wide range of indicators)
7. Dispensing Of The Media :
Cool the agar based medium to 40-50°C and pour as desired.
For Plating : After autoclaving pour into Petri plates, place them in upright position to cool under
laminar flow and then immediately cover with lid.
5. Specialized Media: Specialized media has additives that isolate specific pathogens. It can help
provide insight to colony morphology, differentiation and identification of these pathogens. Mueller
Hinton Media (TM 339) is an example of this type of media. It was originally designed to isolate
pathogenic Neisseria, but is now universally used for antibiotic susceptibility testing as per CLSI
standards.
1. Carefully read instruction for use on labels.
2. Note the Best Before and Lot /Batch No. for your records. Some media may have shorter shelf
lives than other depending upon the product.
3. Write on the label the date of receipt in the laboratory.
4. Store as indicated on the labels. as it is hygroscopic in nature, usually it is specified to keep usually
below 25°C in dry area, away from direct sunlight, autoclaves, drying oven and other heat
sources.
5. Ensure the proper capping of the box after every use.
6. Kindly consult Material Safety Data Sheet for hazardous products.
1. Complete instructions for the preparation of culture media are given on labels of each bottles.
2. Always use rehydrated, clean, undamaged glassware and fresh prepared distilled or deionized
water.
3. Place appropriately weighed amount in clean dry flask, 2-3 times larger than the final volume of
prepared medium.
4. Add part of the required amount of distilled water and swirl to dissolve. Now add remaining water
from the side of container with gentle heating using hot plate, open fame, water bath, microwave
(Should not be kept for longer period). While performing it one should avoid overheating,
scorching or burning.
5. pH Adjustment: The pH value of the media shall produce the equivalent value at 25°C as
mentioned on the label. For best results, prepare the medium with distilled or deionised water
only. If old material is being used, it is recommended to check the pH and correct if necessary
before use. pH adjustment (if required) should be carried out with 0.1 N Hydrochloric Acid or
Sodium Hydroxide Solution.
6. Sterilization: Generally, sterilization is done at 121°C for 15 mins at 15 psi using autoclave.
Volume larger than 2 ltr may require more autoclaving time to achieve proper sterilization. Colour
of the media may vary from other brands but the growth of microorganisms will be same as
desired levels.
STANDARD OPERATING PROCEDURE
RECONSTITUTION OF DEHYDRATED MEDIA
Note: Open the culture medium container away from draughts and moisture. Avoid inhaling the powder and
prolonged skin contact.

For Broth : Sterile broth may be cooled to room temperature or laminar flow as desired.
For Slant and Motility prepared Media: After dissolving the media, dispense appropriate quantity
(8ml) into the tubes and then autoclave. Remove from autoclave and keep in slanted position to solidify.
Note : Any heat labile or heat sensitive enrichments or supplements must be added aseptically after lowering the
temperature of agar to 40-50°C and then poured as desired.
If the prepared media are not to be used within 24 hours then these should be stored at low
temperature i.e. 2-8°C in moisture proof container. Do not refrigerate the medium. Petri plates should
be kept in upright position. Also, it is recommended to use Paraffin and then to keep in plastic zip bags
so that it does not retain moisture.
A microbiological culture or microbial culture, is a method of multiplying microbial organisms by
letting them reproduce in predetermined culture medium under controlled laboratory conditions.
Whereas, a pure (or axenic) culture is a population of cells or multicellular organisms growing in the
absence of other species or types.
There are number of procedures available for the isolation of pure cultures from mixed populations A
pure culture may be isolated by the use of special media with specific chemical or physical agents that
allows the enrichment or selection of one organism over another. The method of isolation includes:
1. Pour Plating or Spread Plating Technique
2. Streak plating with quantified loop.
1. Prepare serial dilutions* of autoclaved distilled or deionized water as shown in Figure 1.
STORAGE OF PREPARED MEDIA
METHOD OF ISOLATION
POUR PLATING
*Serial Dilution : A serial dilution is the stepwise dilution of a substance in solution. 1ml / 1 gm from
master sample is transferred to tube containing 9ml autoclaved distilled or deionized water. Then
transfer from first tube to second and so on. Be sure to mix the distilled or deionized water in each tube
before each serial transfer.
Calculation: Number of colonies on plate x reciprocal of dilution of sample = number of bacteria/ml
(for example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10, 000 = 320,000 /ml in sample.)
2. Inoculate labelled empty petri dish with specified mL (0.1 or 1.0 mL) of diluted specimen.
3. Collect one bottle of sterile molten agar. Hold the bottle in the right hand; remove the cap with the
little finger of the left hand.
4. Flame the neck of the bottle.
1:10 1:100 1:1000 1:10,000 1:100,000
1 ml 1 ml 1 ml 1 ml 1 ml
Original
Inoculum
Dilutions 1:10 1:100 1:1000 1:10,000 1:100,000
Plating
Figure 1 : Serial Dilution

5. Lift the lid of the Petri dish slightly with the left hand and pour the sterile molten agar into the Petri dish
and replace the lid.
6. Flame the neck of the bottle and replace the cap.
7. Gently rotate the dish to mix the culture and the medium thoroughly and to ensure that the medium
covers the plate evenly. Do not slip the agar over the edge of the petri dish.
8. Allow the agar to completely gel without disturbing it, it will take approximately 10 minutes.
9. Seal and incubate the plate in an inverted position at 37°C for 24-48 hours.
1. Prepare serial dilutions of the autoclaved distilled or deionized water as shown in Figure 1.
2. Pipette out 0.1 ml from the appropriate desired dilution series onto the centre of the surface of an agar
plate.
3. Dip the L-shaped glass spreader into alcohol.
4. Flame the glass spreader (hockey stick) over a Bunsen burner.
5. Spread the sample evenly over the surface of agar using the sterile glass spreader, carefully rotating
the Petri dish underneath at the same time.
6. Incubate the plate at 37°C for 24 hours.
SPREAD PLATING
Figure 2 : Step 1- Spread Plate Method
Step 2- Pour Plate Method
Incubation
Colonies grow in and
on solidified medium
Incubation
Colonies grow on a
surface of medium
Solid Agar
Medium
Spread inoculum over surface evenly
Add melted agar medium
& swirl to mix
0.1 ml
Molten
agar medium
1ml or 0.1ml
Diluted
bacterial
suspension
Inoculate empty plate
Inoculate plate containing
solid medium
Step 2
Step 1
Streak Plate Technique
Streaking is a technique used to isolate a pure strain from a single species of microorganism, mostly
bacteria. This technique is also called as “picking colonies” which useful when you need to separate
organisms in a mixed culture or when you need to study the colony morphology of an organism. Usually
by the third or fourth quadrant only a few organisms are transferred which will give discrete colony
forming units (CFUs).
1. Sterilize the inoculating loop in the bunsen burner by putting the loop into the flame until it is red hot.
Allow it to cool.
2. Pick an isolated colony from the agar plate culture and spread it over the first quadrant using close
parallel streaks.
3. Immediately streak the inoculating loop very gently over a quarter of the plate using a back and forth
motion.
4. Flame the loop again and allow it to cool. Going back to the edge of area 1 that you just streaked,
extend the streaks into the second quarter of the plate.
5. Similarly, extend the streaks into the third quarter and fourth quarter of the plate.

Alkaline glassware, overheating, incomplete mixing, impure water,
repeated remelting, prolonged storage at high temperature, pH taken at
wrong temperature, pH equipment faulty or poorly standardized.
Incorrect pH
FAULTS CAUSES
Impure water, dirty glassware, deterioration of dehydrated medium, excess
heat, wrong pH.
Abnormal Colour
Inadequate heating of agar media, incomplete mixing, too small container,
impure water and glassware.
Incomplete Solubility
Undissolved agar, pH too low causing acid hydrolysis, inaccurate weighing,
inadequate mixing, excess heat, incomplete reconstitution of dehydrated
medium.
Soft Gel
Overheating, deterioration of dehydrated medium, improper mixing,
excess amount of dehydrated powder.
Darkening
Excessive reheating, incomplete mixing, ailure to compensate for dilution
of ingredients, repeated melting, disturbance in the formula by the
inoculum carriers, etc.
Loss of Growth
Promoting Property
Scorching or burning, deterioration of dehydrated medium, impure water
or glassware.
Toxicity
Improper sterilization, poor techniques.Contamination
Disposal of Media:
All samples and cultures should be handled carefully and should not be discard without autoclaving.
These should be discarded after autoclaving at 15 psi (121°C) for 20 minutes.
0
0
0
0
0
0
0
0
0
1. Read instructions carefully given over the labels and note the best before date of each lot
before use.
2. Confirm the physical characteristics of Dehydrated Culture Media, it should be
homogenous and free flowing.
3. Since the Culture Media are highly hygroscopic, store them in cool (preferred below
25°C, unless and otherwise specified) and dry place. Protect it from direct sunlight and
humid place.
4. Ensure proper capping box after use.
5. Efficiency of the autoclave should be ascertained from time to time using various physical
measurements or Biological / Chemicals indicators.
6. The petri plates should be sterilized by keeping washed and dried plates in oven at 150°C
for 2 hours.
7. Never store the prepared media at 0°C.
8. Bring the stored media to room temperature before use or as per instructions for use.
9. Take care while using hazardous chemicals and use methods which reduce the risk of
inhalation, ingestion and contact with skin, eyes and clothing. To avoid mishappening
wear protective clothing and equipment's.
11. Avoid contaminated apparatus and glassware.
12. Do not eat, drink or smoke while handling and using chemicals.
13. Wash hands and exposed areas thoroughly and change contaminated clothings.
14. Consult doctor immediately if affected by chemicals and use appropriate first aid.
TROUBLESHOOT GUIDE
SAFETY MEASURES

1
A
AK AGAR NO. 2
(SPORULATING AGAR)
(ARRET AND KIRSHBAUM MEDIUM)
for production of spores of Bacillus subtilis ATCC 6633 which are used as inoculum in detection of
Penicillin and other antibiotics
500 gm30.80TM 1326
AEROMONAS PSEUDO SELECTIVE AGAR
for detecting Pseudomonas and Aeromonas in foodstuffs and waste water
500 gm44.86TM 1482
AEROMONAS STARCH DNA AGAR BASE
for selective isolation and enumeration of Aeromonas species from food and clinical samples
AMPICILLIN SUPPLEMENT 0
(Store below 2-8 C)
500 gm
5 vl
52.00
#10 vl
TM 1137
TS 097
AEROMONAS ISOLATION MEDIUM BASE
for selective & differential isolation of Aeromonas hydrophila from clinical & environmental samples
0
AEROMONAS SELECTIVE SUPPLEMENT (Store below 2-8 C)
500 gm
5 vl
56.30
#18 vl
TM 1136
TS 099
ACTINOMYCES BROTH
for cultivation and maintenance of anaerobic Actinomyces species
500 gm57.22TM 1134
ACTINOMYCES AGAR
for cultivation and maintenance of anaerobic Actinomyces species
500 gm77.22TM 1133
ACETOBACTER AGAR W/LIVER EXTRACT
for maintenance of dextrose positive Acetobacter species
500 gm57.00TM 1131
ACETOBACTER AGAR (MANNITOL)
for maintenance of mannitol positive Acetobacter species
500 gm48.00TM 1130
ACETOBACTER AGAR (GLUCOSE)
for maintenance of dextrose positive Acetobacter species
500 gm38.00TM 1129
ACETATE DIFFERENTIAL AGAR (SIMMON’S CITRATE AGAR, MODIFIED)
for differentiation of Shigella species & Escherichia coli gram microorganismsnegative non-fermentative
500 gm29.18TM 006
0
ACETATE AGAR (Store below 8 C)
for the isolation and cultivation of Leuconostoc and Pediococcus species
500 gm61.90TM 1324
ACETAMIDE NUTRIENT BROTH (DOUBLE PACK)
for detection of utilization of acetamide by Pseudomonas aeruginosa
(Part I)
(Part II)
100 gm
500 gm
0.56
2.00
TM 1128
ACETAMIDE BROTH (DOUBLE PACK) (Part I)
for differentiation of non-fermentative gram negative bacteria like Pseudomonas aeruginosa (Part II)
100 gm
500 gm
10.00
7.63
TM 351
AC BROTH
for cultivation of aerobes and sterility testing of biological products without mercurial preservatives
500 gm34.20TM 003
AC AGAR
for cultivation of various microorganisms especially for sterility testing
500 gm35.20TM 002
A-1 MEDIUM
for determination of faecal coliforms in water and foods by MPN technique
100 gm
500 gm
31.50TM 001
ACETAMIDE AGAR (DOUBLE PACK) (IS0)
for confirmation of Pseudomonas aeruginosa in water samples (Part II)
(Part I) 500 gm10.00
22.63
TM 401
500 gm22.00ACTINOMYCETE ISOLATION AGAR
for isolation and propagation of Actinomycetes from soil and water
TM 007
ALOA LISTERIA AGAR BASE (L. MONO DIFFERENTIAL AGAR BASE)
0
L.MONO SELECTIVE SUPPLEMENT I (Store below 8 C)
0
L.MONO SELECTIVE SUPPLEMENT II (Store below 8 C)
0
L.MONO ENRICHMENT SUPPLEMENT I (Store below 8 C)
for selective and differential isolation of Listeria monocytogenes
100 gm
500 gm
5 vl
5 vl
5 vl
72.00
#14 vl
#14 vl
#14 vl
TM 1443
TS 227
TS 228
TS 229
ALEKSANDROW AGAR
For isolation and detection of potassium solubilizing bacteria from soil sample
500 gm29.60TM 1889
ALEKSANDROW BROTH
For enrichment of potassium solubilizing bacteria from soil samples
500 gm9.60TM 1890
NEW
NEW

A
2
100 gm
500 gm
15.00ALKALINE PEPTONE WATER (BIS)
for enrichment of Vibrio species
TM 649
100 gm
500 gm
20.00ALKALINE PEPTONE WATER (pH 8.0) (VEG.)
TMV 352
100 gm
500 gm
20.00ALKALINE PEPTONE WATER (pH 8.0)
TM 352
500 gm50.00ALKALINE PEPTONE WATER (pH 8.6) (ISO)
for detection of Vibrio panahaemolyticus species
TM 008
100 gm
500 gm
1.87ALGAE CULTURE BROTH
for isolation and cultivation of algae from soil and water
TM 648
500 gm16.87ALGAE CULTURE AGAR
for isolation and cultivation of algae from soil and water
TM 647
100 gm
500 gm
15.10ANDRADE PEPTONE WATER (VEG.)
a basal medium to study fermentation reactions by adding carbohydrates
TMV 012
100 gm
500 gm
15.10ANDRADE PEPTONE WATER
TM 012
500 gm50.40ANAEROBIC TRYPTONE SOYA AGAR
for screening anaerobes in cosmetic products like talcum powder
TM 557
500 gm40.50ANAEROBIC THIOGLYCOLLATE MEDIUM BASE
for cultivation of anaerobes
TM 1499
500 gm40.00ANAEROBIC FERMENTATION MEDIUM BASE
for detection of fermentation reactions of anaerobic microorganisms
TM 651
500 gm35.40ANAEROBIC BASAL BROTH
for cultivation of anaerobic microorganisms like Bacteroides and other fastidious anaerobes
TM 1498
500 gm46.00ANAEROBIC BASAL AGAR
for cultivation of anaerobic microorganisms, like Bacteroides and other fastidious anaerobes
TM 1497
500 gm
5 vl
40.00
#13 vl
ANAEROBIC BLOOD AGAR BASE
for isolation and cultivation of Group A and B Streptococci from clinical samples
0
NEOMYCIN SUPPLEMENT (Store below 8 C)
TM 927
TS 095
500 gm53.00ANAEROBIC AGAR (BREWER)
for isolation and sensitivity testing of anaerobic and microaerophilic organisms
TM 011
100 gm
500 gm
58.00ANAEROBIC AGAR
for cultivation of anaerobic bacteria especially Clostridium species
TM 010
500 gm26.50AMMONIUM PHOSPHATE AGAR
for detection of the ability of microorganisms to utilize ammonium phosphate as nitrogen source
TM 556
100 gm
500 gm
20.00AMIES TRANSPORT MEDIUM W/ CHARCOAL
for transportation and preservation of bacteriological samples
TM 009
100 gm
500 gm
10.00AMIES TRANSPORT MEDIUM W/O. CHARCOAL
for transportation and preservation of bacteriological samples
TM 456
100 gm
500 gm
29.00ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per IP) (VEG.)
for sterility testing of biological products
TMV 302
100 gm
500 gm
29.00ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per IP)
TM 302
100 gm
500 gm
28.50ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per USP) (VEG.)
TMV 301
100 gm
500 gm
28.50ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per USP)
TM 301
500 gm25.01ANDRADE LACTOSE PEPTONE WATER
to study fermentation reactions of members of the Enterobacteiaceae
TM 1797
ANDRADE 0.5% LACTOSE PEPTONE WATER
for determination of coliform bacteria particularly Enterobacteriaceae on the basis of their ability to
ferment lactose
TM 1859 100 gm
500 gm
15.01
100 gm
500 gm
40.00ALKALINE SALINE PEPTONE WATER (ASPW)
for enrichment of Vibrio species from food and water samples in accordance with ISO
(ISO)TM 1878

3
A
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO. 8 (as per USP)
for microbiological assay of Mitomycin, Plicamycin and Vancomycin
TM 1743
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO. 8 (BASE AGAR W/ LOW pH) (VEG.)
TMV 019
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO. 8 (BASE AGAR W/ LOW pH)
TM 019
500 gm30.00ANTIBIOTIC ASSAY MEDIUM NO. 6 (VEG.)
for induction of spore production in Bacillus subtilis strains used in antibiotic assay
TMV 018
500 gm30.00ANTIBIOTIC ASSAY MEDIUM NO. 6
for induction of spore production in Bacillus subtilis strains used in antibiotic assay
TM 018
500 gm25.50ANTIBIOTIC ASSAY MEDIUM E (as per IP)
for microbiological assay of Framycetin and Kanamycin using Bacillus subtilis
TM 1740
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO. 5 (STREPTOMYCIN ASSAY AGAR) (VEG.)
for microbiological assay of Streptomycin using Bacillus subtilis
TMV 017
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO. 5 (STREPTOMYCIN ASSAY AGAR W/ YEAST EXTRACT) (as per IP/ USP)
for microbiological assay of Streptomycin using Bacillus subtilis
TM 017
for detection of Penicillin in milk and for microbiological assay of different antibiotics
TM 1737
500 gm26.50ANTIBIOTIC ASSAY MEDIUM NO.4 (VEG.)
detection of Penicillin-G in milk using Bacillus stearothermophilus
TMV 427
500 gm26.50ANTIBIOTIC ASSAY MEDIUM NO. 4 (YEAST MEAT AGAR)
for detection of Penicillin-G in milk using Bacillus stearothermophilus
TM 427
100 gm
500 gm
17.50ANTIBIOTIC ASSAY MEDIUM C (as per IP)
for turbidimetric or serial dilution assay of various antibiotics
TM 1735
100 gm
500 gm
17.50ANTIBIOTIC ASSAY MEDIUM NO. 3 (as per USP)
for turbidimetric or serial dilution assay of various antibiotics
TM 1734
100 gm
500 gm
17.50ANTIBIOTIC ASSAY MEDIUM NO. 3 (ASSAY BROTH) (VEG.)
for microbiological assay of antibiotics
TMV 015
100 gm
500 gm
17.50ANTIBIOTIC ASSAY MEDIUM NO. 3 (ASSAY BROTH)
TM 015
500 gm25.50ANTIBIOTIC ASSAY MEDIUM B (as per IP)
TM 1732
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO.2 (as per USP)
TM 1731
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO.2 (BASE AGAR) (VEG.)
TMV 359
500 gm25.50ANTIBIOTIC ASSAY MEDIUM NO.2 (BASE AGAR)
TM 359
100 gm
500 gm
30.50ANTIBIOTIC ASSAY MEDIUM NO.1 (as per USP)
for microbiological assay of â-lactam & other antibiotics
TM 1729
100 gm
500 gm
30.50ANTIBIOTIC ASSAY MEDIUM NO.1 (SEED AGAR) (VEG.)
for microbiological assay of â-lactam and other antibiotics
TMV 390
100 gm
500 gm
30.50ANTIBIOTIC ASSAY MEDIUM NO.1 (SEED AGAR)
for microbiological assay of â-lactam and other antibiotics
TM 390
500 gm18.10ANDRADE PEPTONE WATER W/ MEAT EXTRACT
a basal medium to study fermentation reactions of members of the Enterobacteriaceae
TM 652
100 gm
500 gm
15.10ANDRADE PEPTONE WATER (BIS)
TM 936

A
4
ANTIBIOTIC ASSAY MEDIUM NO. 19 (as per USP)
for microbiological assay of Amphotericin B, Netamycin & Nystatin using Saccharomyces cerevisiae
500 gm60.00TM 1755
ANTIBIOTIC ASSAY MEDIUM F (as per IP)
for microbiological assay of Tetracycline & Oxytetracyline
500 gm25.50TM 1744
ANTIBIOTIC ASSAY MEDIUM NO. 9 (POLYMYXIN BASE AGAR)
for assaying the products containing Polymyxin-B
500 gm50.00TM 020
ANTIBIOTIC ASSAY MEDIUM NO. 9 (POLYMYXIN BASE AGAR (VEG.)
for assaying the products containing Polymyxin-B
500 gm50.00TMV 020
for microbiological plate assay of Carbenicillin, Colistimethate sodium and Polymyxin-B
500 gm50.00TM 1746
ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (Part I)
for assay of Polymyxin-B, Carbenicillin, Colistin and Colistimethate sodium (Part II)
500 gm42.00
Polysorbate
80
TM 021
ANTIBIOTIC ASSAY MEDIUM NO. 11 (NEOMYCIN, ERYTHROMYCIN ASSAY AGAR)
100 gm
500 gm
30.50TM 022
ANTIBIOTIC ASSAY MEDIUM NO. 11 (NEOMYCIN, ERYTHROMYCIN ASSAY AGAR) (VEG.)
100 gm
500 gm
30.50TMV 022
100 gm
500 gm
30.50TM 1751
ANTIBIOTIC ASSAY MEDIUM D (as per IP)
100 gm
500 gm
30.50TM 653
ANTIBIOTIC ASSAY MEDIUM NO. 12 (NYSTATIN ASSAY AGAR)
for microbiological assay of Amphotericin B & Nystatin using Saccharomyces cerevisiae
500 gm62.50TM 023
ANTIBIOTIC ASSAY MEDIUM NO. 12 (NYSTATIN ASSAY AGAR) (VEG.)
for microbiological assay of Amphotericin B & Nystatin using Saccharomyces cerevisiae
500 gm62.50TMV 023
ANTIBIOTIC ASSAY MEDIUM NO. 13 (NYSTATIN ASSAY BROTH)
for microbiological assay of Candicidin using Saccharomyces cerevisiae
500 gm30.00TM 024
ANTIBIOTIC ASSAY MEDIUM NO. 13 (NYSTATIN ASSAY BROTH) (VEG.)
for microbiological assay of Candicidin using Saccharomyces cerevisiae
500 gm30.00TMV 024
for turbidimetric microbiological assay of Candicidin using Saccharomyces cerevisiae and to study
the effectiveness of antibiotics on yeasts and molds
500 gm30.00TM 1753
ANTIBIOTIC ASSAY MEDIUM NO. 19
500 gm60.00TM 025
ANTIBIOTIC ASSAY MEDIUM NO. 19 (VEG.)
500 gm60.00TMV 025
ANTIBIOTIC ASSAY MEDIUM G (as per IP)
for microbiological assay of Amphotericin B, and Netamycin using Saccharomyces cerevisiae
500 gm60.00TM 1757
ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (VEG.) (Part I)
(Part II)for assay of Polymyxin-B, Carbenicillin, Colistin and Colistimethate sodium
500 gm42.00
Polysorbate
80
TMV 021
for antibiotic assay of Carbenicillin, Colistimethate sodium and Polymyxin-B (Part II)
(Part I) 500 gm42.00
Polysorbate
80
TM 1748
ANTIBIOTIC ASSAY MEDIUM-H (as per IP)
for microbiological plate assay of Carbenicillin and Polymyxin-B (Part II)
(Part I) 500 gm42.00
Polysorbate
80
TM 1749
ANTIBIOTIC ASSAY MEDIUM NO. 20 (YEAST BEEF BROTH)
for microbiological assay of Amphotericin B using Candida tropicalis
500 gm42.50TM 026
ANTIBIOTIC ASSAY MEDIUM NO. 20 (YEAST BEEF BROTH) (VEG.)
for microbiological assay of Amphotericin B using Candida tropicalis
500 gm42.50TMV 026

5
A 500 gm30.80ANTIBIOTIC ASSAY MEDIUM NO. 32
for microbiological assay of Dihydrostreptomycin and Vancomycin by preparing inoculum of
Bacillus subtilis ATCC 6633
TM 654
Bacillus subtilis ATCC 6633
for microbiological assay of Dihydrostreptomycin and Vancomycin by preparing inoculum of
TMV 654
for assay of dihydrostreptomycin and Vancomycin by plate assay method by preparing inoculum of
Bacillus subtilis
TM 1760
for microbiological assay of Bleomycin by using Mycobacterium smegmatis
TM 655
TM 1761
for microbiological assay of Bleomycin using Mycobacterium smegmatis
TM 656
for microbiological assay of Bleomycin using Mycobacterium smegmatis
TMV 656
500 gm40.00ANTIBIOTIC ASSAY MEDIUM NO, 35 (as per USP)
TM 1763
500 gm40.00ANTIBIOTIC ASSAY MEDIUM I (as per IP)
TM 1764
100 gm
500 gm
40.00ANTIBIOTIC ASSAY MEDIUM NO. 36 (SOYA BEAN CASEIN DIGEST AGAR) (TRYPTONE SOYA AGAR) (as per USP)
for isolation of various fastidious microorganisms with or without added blood
TM 345
100 gm
500 gm
40.00ANTIBIOTIC ASSAY MEDIUM NO. 36 (SOYA BEAN CASEIN DIGEST AGAR) (TRYPTONE SOYA AGAR) (as per USP) (VEG.)
for isolation of various fastidious microorganisms with or without added blood
TMV 345
100 gm
500 gm
40.00ANTIBIOTIC ASSAY MEDIUM J (as per IP)
for cultivation of various microorganisms and for sterility testing in pharmaceutical procedures
TM 1767
100 gm
500 gm
40.00ANTIBIOTIC ASSAY MEDIUM J (as per IP) (VEG.)
for cultivation of various microorganisms and for sterility testing in pharmaceutical procedures
TMV 1767
100 gm
500 gm
30.00ANTIBIOTIC ASSAY MEDIUM NO. 37 (SOYA CASEIN DIGEST MEDIUM) (TRYPTONE SOYA BROTH)
for sterility testing and cultivation of fastidious and non-fastidious microorganisms
(ISO)TM 332
100 gm
500 gm
30.00ANTIBIOTIC ASSAY MEDIUM NO. 37 (SOYA CASEIN DIGEST MEDIUM) (TRYPTONE SOYA BROTH) (VEG.)
for sterility testing and cultivation of fastidious and non-fastidious microorganisms
TMV 332
100 gm
500 gm
30.00ANTIBIOTIC ASSAY MEDIUM NO. 37 (as per USP)
for sterility testing of molds and cultivation of various microorganisms
TM 1476
for microbiological assay of Ticarcillin using Pseudomonas aeruginosa
TM 658
TMV 658
TM 1770
for microbiological assay of Neomycin and Streptomycin using Klebsiella pneumoniae
TM 659
for microbiological assay of Thiostrepton using Streptococcus faecium (Enterococcus faecium)
TM 660
for microbiological assay of Neomycin using Klebsiella pneumoniae
TM 1772
for microbiological assay of Neomycin using Klebsiella pneumoniae
TMV 659

A
6
microbiological assay of Thiostrepton using Streptococcus faeciumfor
500 gmTMV 660 47.10
for microbiological assay of Thiostrepton using Streptococcus faecium
500 gmTM 1774 47.10
500 gmTM 1776 46.00
ANTIBIOTIC ASSAY MEDIUM NO. 41
500 gmTM 661 46.00
500 gmTMV 661 46.00
ANTIBIOTIC SULPHONAMIDE SENSITIVITY TEST AGAR (ASS AGAR)
for testing antimicrobial activity of antibiotics and sulphonamides and also for detecting the
presence of antimicrobial substances
500 gmTM 1460 40.04
ANTIFUNGAL ASSAY AGAR
for assay of antifungal activity in pharmaceutical and other products by cylinder plate/disc method
500 gmTM 027 75.76
APRY AGAR BASE
for detection and isolation of acid resistant yeasts, Zygosaccharomyces bacillii and
Zygosaccharomyces rouxii in food products
POTASSIUM SORBATE 10% (10ml/vl)
500 gm
5 vl
TM 1329
TS 137
107.50
#5 vl
APRY BROTH BASE
for detection and isolation of acid resistant yeasts, Zygosaccharomyces bacillii and
Zygosaccharomyces rouxii in food products
0
CHLORTETRACYCLINE SELECTIVE SUPPLEMENT (Store below 8 C)
500 gm
5 vl
TM 1330
TS 138
82.55
#6 vl
APT AGAR
for cultivation of hetero-fermentative Lactobacilli and other organisms by extra thiamine content
500 gmTM 1323 61.20
APT BROTH
for cultivation of hetero-fermentative Lactic acid bacteria by extra thiamine content
500 gmTM 1462 46.20
ARABINOSE AGAR BASE
for
0
ENTEROCOCCUS FAECIUM SELECTIVE SUPPLEMENT (Store below 8 C)
differentiation between Enterococcus faecium & Streptococcus faecalis
500 gm
5 vl
TM 1470
TS 168
54.10
#19 vl
ARGININE DIHYDROLASE BROTH
for detection of Arginine dehydrolase producing microorganisms
500 gmTM 1140 19.30
L-ARGININE DIHYDROLASE MEDIUM, MODIFIED (ISO)
for confirmation of Enetrococcus sakazakii from milk and milk products
500 gmTM 1505 9.01
ASCOSPORE AGAR
for detection of ascosporogenous yeasts
500 gmTM 1141 43.50
ASHBY’S GLUCOSE AGAR
for cultivation of Azotobacter species by using glucose as carbon source
500 gmTM 662 40.70
ASHBY’S MANNITOL AGAR
for isolation of Azotobacter species from soil
500 gmTM 663 40.70
ASLA AGAR BASE
PROPIONIBACTERIA GROWTH SUPPLEMENT
for selective isolation and cultivation of Propionibacterium species
500 gm
5 vl
TM 1331
TS 139
16.20
#62 vl
ASPARAGINE BROTH
for identification and enumeration of Pseudomonas
0
(Store below 8 C) 100 gm
500 gm
TM 664 28.00
ASPARAGINE GELATIN LACTATE MEDIUM BASE
for isolation of sulphur bacteria
100 gmTM 665 152.00
ASPARAGINE NITRATE MEDIUM
for cultivation and isolation of denitrifying bacteria from soil
100 gmTM 599 27.70
ASPARAGINE PROLINE BROTH (VEG.)
for cultivation of Pseudomonas aeruginosa from water by membrane filter technique
100 gm
500 gm
14.50TMV 666
ASPARAGINE PROLINE BROTH
for cultivation of Pseudomonas aeruginosa from water by membrane filter technique
100 gm
500 gm
14.50TM 666

TM Media products Catalog 2018 | Products List

TM Media products Catalog 2018 | Products List

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TM Media products Catalog 2018 | Products List