Medical Microbiology Lecture

2. 
Restriction endonuclease,



2
vds

3. 










3
vds

4. 
1 Southern blotting – Detection of DNA fragments
2 Northern Blotting – Detection of RNA fragments
3 Western blotting – Detection of Proteins
4 Eastern Blotting – Detection of Post translational
modification (PTM) of Proteins, such as
lipids, carbohydrates or phosphorylation or
any other protein modification
vds 4

5.  1 Southern blotting: Detection of DNA fragments

E. M. Southern




vds 5

6.  1 Southern blotting: Detection of DNA fragments
vds 6

7.  2 Northern blotting: Detection of RNA fragments


vds 7

8.  3 Western blotting: Detection of Proteins
 called Immunoblotting.
 In this, Protein antigen mixture is
separated by SDS-PAGE (Sodium
DedocylSulphate-PolyAcrylamide
Gel Electrophoresis).
 Blotted on Nitrocellulose Membrane
Filter paper and identified by
radiolabeled or enzyme labelled
antibodies as probes.
 Used as confirmatory test in HIV
infection.
 Also to detect TORCH group
infection in pregnant women.
vds 8

9.  4 Eastern blotting: Detection of Proteins Post translational
modification (PTM), to detect lipids, carbohydrates, Glyco-
conjugate or phosphorylation or any other protein modification.

vds 9

10. POLYMERASE CHAIN REACTION (PCR)



10
vds

11. 

11
vds

12.  Thermal Cycler
12
vds

13. 


vds 13

14.  Types of PCR:
 Reverse Transcriptase – PCR (RT-PCR)
 Quantitative PCR (qPCR)
 Nested PCR
 Multiplex PCR
14
vds

15.  GENETIC MAPPING:



15
vds

16. 
16
vds

17. 17
vds

18.  Production of vaccines –
 Production of proteins –
 Gene Therapy –
18
vds

19.  A technique by which a faulty gene is replaced
with normal gene in persons with fatal or
extremely debilitating genetic diseases.
 Types:
1. Ex Vivo gene therapy
2. In Vivo gene therapy
19
vds

20. 20
vds