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TERM PAPER-I PRESENTATION
ON
INSECT NERVOUS SYSTEM AND IMPULSE
TRANSMISSION
Course In-Charge
ENTO 606
Dr.G.Sridevi, PS & Head (AINP on PR)
Dr.K.Kavitha, Senior Scientist (AINP on PR)
SABHAVAT SRINIVASNAIK
ID.No: RAD/2021-25
In-Service I Ph.D
DEPARTMENT OF PATHOLOGY
COLLEGE OF AGRICULTURE, RAJENDRANAGAR
PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY
Preamble
Axonic transmission
Synoptic transmission
Insect nervous system structure
CONTENTS
Insecticide molecules and Target
Insect Nervous system function
References
Summary & Conclusion
PREAM
PREAMBLE
 Protozoa to Chordata- highest evolved
organism- human being
 A unicellular organism-single cell-
functions- less coordination
 A multicellular organism-well organised
mass of matter
 Contains different chemical compounds
 It performs chemical activities and physical
activities
 Charged organism–state of equilibrium with
its environment.
 “Coordination-Insect nervous system”
 Nervous system- poorly insulated current
system
 Producing the harmonised behaviour by
coordinating 300 skeletal muscles
 Temporal and spatial manner
 Insecticides act on nervous system
Chapman,1983 & Simpson, 2013
Structural and Functional Unit: Neuron
STRUCTURE
Neuron
Glial cells
Nervous system
INSECT NERVOUS SYSTEM STRUCTURE-NEURON
Soma/Cell body/Perikaryon
Nucleus
Dendrite
Axon
Glial cells-N.lamella
Protoplasm
Collateral
Arborisations
Stimuli
Neurons-ganglion
Branched projections of a neuron
that conduct the impulses received
from other neural cells to the cell
body
Long slender projection of a nerve cell
that conducts nerve impulses from the
cell body to other neurons, muscles,
and organs Chapman,1983 & Simpson, 2013
INSECT NERVOUS SYSTEM STRUCTRE-SYNAPSES
INSECT NERVOUS SYSTEM STRUCTRE-SYNAPSES
i) Synaptic cleft-20-25 nm
(Chemical transmission)
Vesicles
ii) Gap junction-3.5 nm
(Direct transmission)
Post synaptic neuron
Pre synaptic neuron
Synapse
The junction between the terminal of a neuron and either another neuron or a
muscle or gland cell, over which nerve impulses pass
 Electron lucent
 Electron dense
 Physical synapses
 Functional synapses
 Retinula cell of fly =200 Physical synapse
 Single to many cells/
 Among them one =functional
INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
 Each neuron consist of glial cells
 Synaptic contact occur where there is no glial cells
 Glial cells pass nutrient materials to neurons
 It reserves glycogen
 Making repairs to the neurons
 Ionic balance and protection- Blood Brain Barrier concept
INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
CLASSIFICATION OF NEURONS -STRUCTURAL BASIS
Neuron with a proximal axon & many distal dendrites
Neuron with a single axon
Neuron with a proximal axon and a long distal dendrite
1
1
2
1
2
3
4
5
6
Internuncial (association) neurons
Unipolar cells (often with several collaterals
and/or branching axons) (conduct signals
within CNS)
Motor neurons: conducts impulse from CNS
to effector organs-Efferent neurons, monopolar,
situated in the ganglia and conduct impulse
from CNS to effector organs like muscles and
glands
Afferent (sensory) neurons
Bipolar or multipolar cells have dendrites -
associated with sense organs or receptors
(carry information towards CNS).
CLASSIFICATION OF NEURONS –FUNCTIONAL BASIS
INSECT NERVOUS SYSTEM
Nervous system divided in to three major sub-systems
1. Central nervous system (CNS)
2. Visceral nervous system (VNS)
3. Peripheral nervous system (PNS)
INSECT NERVOUS SYSTEM-CNS
Contains double series of nerve centers (ganglia).These ganglia are connected
by longitudinal tracts of nerve fibers called connectives. Transverse tracts of
nerve fibers called commissures
INSECT NERVOUS SYSTEM-CNS
1.Brain
Formed by the fusion of first three
cephalic neuromeres
a) Protocerebrum
Large, innervate compound eyes
and ocelli
b) Deutocerebrum:
Found beneath protocerebrum,
innervate antennae
c) Tritocerebrum:
Bilobed, innervate labrum
Main sensory centre controlling
insect behaviour
INSECT NERVOUS SYSTEM-CNS
3.Thoracic ganglia
4. Abdominal ganglia
Median chain of segmental ganglia beneath
oesophagus
Formed by the last three cephalic
neuromeres which innervate mandible,
maxillae and labium.
2. Sub-oesophageal ganglia
Three pairs found in the respective thoracic
segments, largest ganglia, innervate legs
and muscles.
(a)Dictyopterus (Coleoptera: Lycidae) and Pulex (Siphonaptera: Pulicidae)
(b)Blatta (Blattodea: Blattidae) and Chironomus (Diptera: Chironomidae)
(c) Crabro and Eucera (Hymenoptera: Crabronidae and Anthophoridae)
(d)Musca,Calliphora and Lucilia ( Diptera: Muscidae and Calliphoridae)
(e)Hydrometra (Hemiptera: Hydrometridae) and Rhizotrogus (Scarabaeidae).
INSECT NERVOUS SYSTEM-VNS
1. Stomodeal / stomatogastric
Frontal ganglion and connected with
aorta, foregut and midgut
2. Ventral visceral
Associated with the ventral nerve cord
3. Caudal visceral
Associated with the posterior segments
of abdomen and reproductive organs
INSECT NERVOUS SYSTEM-PNS
 Sensory neurons of the cuticular
sensory structures (the sense
organs) that receive mechanical,
chemical, thermal or visual stimuli
from an environment
 All the motor neuron axons that
radiate to the muscles from the
ganglia of the CNS and visceral
nervous system
FUNCTIO
INSECT NERVOUS SYSTEM-IMPULSE TRANSMISSION
A nerve impulse is an electric
current that travels along
dendrites or axon due to ions
moving through voltage gated
channels in the neurons
plasma membrane
Types
1.Axonic conduction
2.Synaptic conduction
IMPULSE TRANSMISSION-AXONIC CONDUCTION
Positive ions
Negative ions
Sodium channels
Axon
Donnan equilibrium
IMPULSE TRANSMISSION-AXONIC CONDUCTION
IMPULSE TRANSMISSION-AXONIC CONDUCTION
Visual/ Chemical/ Mechanical
Sensory neuron
Insect
IMPULSE TRANSMISSION-AXONIC CONDUCTION
Resting neuron
 A charge difference is maintained
 Na+ is out and K+ is in
 Exterior is (+) and interior is (–)
 This is called resting membrane potential
Restoring neuron
 K+ ions comes in (NaK ATPase)
 Na+ ions goes out
 So again interior become (–) and exterior become (+)
IMPULSE TRANSMISSION-SYNAPTIC CONDUCTION
SYNAPTIC CONDUCTION
 Axonal-dendritic (axon to dendrite)
 Axonal-somatic (axon to soma)
 Axonal-axonal (axon to axon)
Location of synapses
Proteins-Acetyl choline
 Synaptotagmin
 Synaptobrevin
 Synap-25
 Syntaxin
IMPULSE TRANSMISSION-SYNAPTIC CONDUCTION
Cholinergic-Acetylcholine
Adrenergic-Norepinephrine
IMPULSE TRANSMISSION-SYNOPTIC CONDUCTION
IMPULSE TRANSMISSION-SYNOPTIC CONDUCTION
DIRECTION OF IMPULSE TRANSMISSION
Dendrite
receives information
Cell body
contains nucleus & organelles
Axon
transmits nerve impulse
axon terminal
transmits to next neuron
Synapse
junction between two neurons
Direction
of
nerve
impulse
INTERACTION OF DIFFERENT N.SYSTEMS
INSECTICIDES & THEIR TARGETS
 DDT
 Synthetic pyrethroids
 Oxadizines
 Cyclodienes- GABA gated Cl channels
 Carbamates- Ach Esterase
 Organophosphates - Ach Esterase
 Neonicotinoids- Nicotine Ach Receptors
 Phenyl pyrazoles- GABA Gated Cl Channel
 Spinosyns-nAchR+GABA Gated Cl Channel
 Avermectins- GABA gated Cl Channel
 Formamidines-Octopamine receptors
Na gated channels
 Diamides –Ryanodine receptors
IMPULSE TRANSMISSIO
AXONIC TRANSMISSION
SYNOPTIC TRANSMISSI
SUMMARY & CONCL
SUMMARY & CONCLUSION
Insect nervous system is responsible for harmonised behaviour of the insect
Neuron is the structural and functional unit-Dendrites, Axons and Synapses
Glial cells are involved in Blood Brain Barrier concept
Axonic and synaptic conduction
2 types of synapses: Cholinergic and Adrenergic
Neurotransmitters, Neuromodulators and Neurohormones
DDT, Synthetic pyrethroids,Oxadizines acts on Na gated channels
Cyclodienes, Carbamates, Organophosphates, Neonicotinoids, Phenyl
pyrazoles, Spinosyns, Avermectins and Formamidines acts on synaptic cleft
REFERENCES
Chapman, R.F.1983.The insects: structure and function, 3rd edn. Cambridge
University Press, Cambridge, 788 pp
Pant, N.C and Swaraj Ghai. 1981. Insect physiology and anatomy, ICAR, New
Delhi
Gerald, K.2002. Cell and Molecular Biology (4th ed.). John Wiley & Sons.864p
Matsumura F.1986.Toxicology of insecticides, 2nd edn. Plenum, New York, 503
pp
Nation, J.L.2001. Insect physiology and biochemistry. Plenum, Boca Raton.
496 pp
Raghumoorthi,K.N.,Balasubramani,V.,Srinivasan,M.R and
Natarajan,N.2011.Insect an Introduction.222P
Scharf, M.E.2003.Neurological effects of insecticides. In:Pimental,D (ed)
Encyclopedia of pest management. Marcel-Dekker, New York.
Srivastava,R.P and Saxena,R.C.1989.Textbook of insect toxicology. Himanshu
publications.166p
THANK YOU

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Insect nervious system and impulse transmission_S.Srinivasnaik_RAD21-25.ppt

  • 1. TERM PAPER-I PRESENTATION ON INSECT NERVOUS SYSTEM AND IMPULSE TRANSMISSION Course In-Charge ENTO 606 Dr.G.Sridevi, PS & Head (AINP on PR) Dr.K.Kavitha, Senior Scientist (AINP on PR) SABHAVAT SRINIVASNAIK ID.No: RAD/2021-25 In-Service I Ph.D DEPARTMENT OF PATHOLOGY COLLEGE OF AGRICULTURE, RAJENDRANAGAR PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY
  • 2. Preamble Axonic transmission Synoptic transmission Insect nervous system structure CONTENTS Insecticide molecules and Target Insect Nervous system function References Summary & Conclusion
  • 4. PREAMBLE  Protozoa to Chordata- highest evolved organism- human being  A unicellular organism-single cell- functions- less coordination  A multicellular organism-well organised mass of matter  Contains different chemical compounds  It performs chemical activities and physical activities  Charged organism–state of equilibrium with its environment.  “Coordination-Insect nervous system”  Nervous system- poorly insulated current system  Producing the harmonised behaviour by coordinating 300 skeletal muscles  Temporal and spatial manner  Insecticides act on nervous system Chapman,1983 & Simpson, 2013 Structural and Functional Unit: Neuron
  • 6. INSECT NERVOUS SYSTEM STRUCTURE-NEURON Soma/Cell body/Perikaryon Nucleus Dendrite Axon Glial cells-N.lamella Protoplasm Collateral Arborisations Stimuli Neurons-ganglion Branched projections of a neuron that conduct the impulses received from other neural cells to the cell body Long slender projection of a nerve cell that conducts nerve impulses from the cell body to other neurons, muscles, and organs Chapman,1983 & Simpson, 2013
  • 7.
  • 8.
  • 9. INSECT NERVOUS SYSTEM STRUCTRE-SYNAPSES
  • 10. INSECT NERVOUS SYSTEM STRUCTRE-SYNAPSES i) Synaptic cleft-20-25 nm (Chemical transmission) Vesicles ii) Gap junction-3.5 nm (Direct transmission) Post synaptic neuron Pre synaptic neuron Synapse The junction between the terminal of a neuron and either another neuron or a muscle or gland cell, over which nerve impulses pass  Electron lucent  Electron dense  Physical synapses  Functional synapses  Retinula cell of fly =200 Physical synapse  Single to many cells/  Among them one =functional
  • 11. INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS  Each neuron consist of glial cells  Synaptic contact occur where there is no glial cells  Glial cells pass nutrient materials to neurons  It reserves glycogen  Making repairs to the neurons  Ionic balance and protection- Blood Brain Barrier concept
  • 12. INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
  • 13. INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
  • 14. INSECT NERVOUS SYSTEM STRUCTURE-GLIAL CELLS
  • 15. CLASSIFICATION OF NEURONS -STRUCTURAL BASIS Neuron with a proximal axon & many distal dendrites Neuron with a single axon Neuron with a proximal axon and a long distal dendrite 1 1 2 1 2 3 4 5 6
  • 16. Internuncial (association) neurons Unipolar cells (often with several collaterals and/or branching axons) (conduct signals within CNS) Motor neurons: conducts impulse from CNS to effector organs-Efferent neurons, monopolar, situated in the ganglia and conduct impulse from CNS to effector organs like muscles and glands Afferent (sensory) neurons Bipolar or multipolar cells have dendrites - associated with sense organs or receptors (carry information towards CNS). CLASSIFICATION OF NEURONS –FUNCTIONAL BASIS
  • 17. INSECT NERVOUS SYSTEM Nervous system divided in to three major sub-systems 1. Central nervous system (CNS) 2. Visceral nervous system (VNS) 3. Peripheral nervous system (PNS)
  • 18. INSECT NERVOUS SYSTEM-CNS Contains double series of nerve centers (ganglia).These ganglia are connected by longitudinal tracts of nerve fibers called connectives. Transverse tracts of nerve fibers called commissures
  • 19. INSECT NERVOUS SYSTEM-CNS 1.Brain Formed by the fusion of first three cephalic neuromeres a) Protocerebrum Large, innervate compound eyes and ocelli b) Deutocerebrum: Found beneath protocerebrum, innervate antennae c) Tritocerebrum: Bilobed, innervate labrum Main sensory centre controlling insect behaviour
  • 20.
  • 21. INSECT NERVOUS SYSTEM-CNS 3.Thoracic ganglia 4. Abdominal ganglia Median chain of segmental ganglia beneath oesophagus Formed by the last three cephalic neuromeres which innervate mandible, maxillae and labium. 2. Sub-oesophageal ganglia Three pairs found in the respective thoracic segments, largest ganglia, innervate legs and muscles.
  • 22. (a)Dictyopterus (Coleoptera: Lycidae) and Pulex (Siphonaptera: Pulicidae) (b)Blatta (Blattodea: Blattidae) and Chironomus (Diptera: Chironomidae) (c) Crabro and Eucera (Hymenoptera: Crabronidae and Anthophoridae) (d)Musca,Calliphora and Lucilia ( Diptera: Muscidae and Calliphoridae) (e)Hydrometra (Hemiptera: Hydrometridae) and Rhizotrogus (Scarabaeidae).
  • 23. INSECT NERVOUS SYSTEM-VNS 1. Stomodeal / stomatogastric Frontal ganglion and connected with aorta, foregut and midgut 2. Ventral visceral Associated with the ventral nerve cord 3. Caudal visceral Associated with the posterior segments of abdomen and reproductive organs
  • 24. INSECT NERVOUS SYSTEM-PNS  Sensory neurons of the cuticular sensory structures (the sense organs) that receive mechanical, chemical, thermal or visual stimuli from an environment  All the motor neuron axons that radiate to the muscles from the ganglia of the CNS and visceral nervous system
  • 26. INSECT NERVOUS SYSTEM-IMPULSE TRANSMISSION A nerve impulse is an electric current that travels along dendrites or axon due to ions moving through voltage gated channels in the neurons plasma membrane Types 1.Axonic conduction 2.Synaptic conduction
  • 27. IMPULSE TRANSMISSION-AXONIC CONDUCTION Positive ions Negative ions Sodium channels Axon Donnan equilibrium
  • 29. IMPULSE TRANSMISSION-AXONIC CONDUCTION Visual/ Chemical/ Mechanical Sensory neuron Insect
  • 30. IMPULSE TRANSMISSION-AXONIC CONDUCTION Resting neuron  A charge difference is maintained  Na+ is out and K+ is in  Exterior is (+) and interior is (–)  This is called resting membrane potential Restoring neuron  K+ ions comes in (NaK ATPase)  Na+ ions goes out  So again interior become (–) and exterior become (+)
  • 32. SYNAPTIC CONDUCTION  Axonal-dendritic (axon to dendrite)  Axonal-somatic (axon to soma)  Axonal-axonal (axon to axon) Location of synapses Proteins-Acetyl choline  Synaptotagmin  Synaptobrevin  Synap-25  Syntaxin
  • 36. DIRECTION OF IMPULSE TRANSMISSION Dendrite receives information Cell body contains nucleus & organelles Axon transmits nerve impulse axon terminal transmits to next neuron Synapse junction between two neurons Direction of nerve impulse
  • 38. INSECTICIDES & THEIR TARGETS  DDT  Synthetic pyrethroids  Oxadizines  Cyclodienes- GABA gated Cl channels  Carbamates- Ach Esterase  Organophosphates - Ach Esterase  Neonicotinoids- Nicotine Ach Receptors  Phenyl pyrazoles- GABA Gated Cl Channel  Spinosyns-nAchR+GABA Gated Cl Channel  Avermectins- GABA gated Cl Channel  Formamidines-Octopamine receptors Na gated channels  Diamides –Ryanodine receptors
  • 43. SUMMARY & CONCLUSION Insect nervous system is responsible for harmonised behaviour of the insect Neuron is the structural and functional unit-Dendrites, Axons and Synapses Glial cells are involved in Blood Brain Barrier concept Axonic and synaptic conduction 2 types of synapses: Cholinergic and Adrenergic Neurotransmitters, Neuromodulators and Neurohormones DDT, Synthetic pyrethroids,Oxadizines acts on Na gated channels Cyclodienes, Carbamates, Organophosphates, Neonicotinoids, Phenyl pyrazoles, Spinosyns, Avermectins and Formamidines acts on synaptic cleft
  • 44. REFERENCES Chapman, R.F.1983.The insects: structure and function, 3rd edn. Cambridge University Press, Cambridge, 788 pp Pant, N.C and Swaraj Ghai. 1981. Insect physiology and anatomy, ICAR, New Delhi Gerald, K.2002. Cell and Molecular Biology (4th ed.). John Wiley & Sons.864p Matsumura F.1986.Toxicology of insecticides, 2nd edn. Plenum, New York, 503 pp Nation, J.L.2001. Insect physiology and biochemistry. Plenum, Boca Raton. 496 pp Raghumoorthi,K.N.,Balasubramani,V.,Srinivasan,M.R and Natarajan,N.2011.Insect an Introduction.222P Scharf, M.E.2003.Neurological effects of insecticides. In:Pimental,D (ed) Encyclopedia of pest management. Marcel-Dekker, New York. Srivastava,R.P and Saxena,R.C.1989.Textbook of insect toxicology. Himanshu publications.166p