Zhen Zeng (University of Helsinki): Patterns of genomic variations in multiple isolates of Heterobasidion parviporum: Impact on the evolution of virulence & pathogenicity traits
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Zhen Zeng (University of Helsinki): Patterns of genomic variations in multiple isolates of Heterobasidion parviporum: Impact on the evolution of virulence & pathogenicity traits
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Zhen Zeng (University of Helsinki): Patterns of genomic variations in multiple isolates of Heterobasidion parviporum: Impact on the evolution of virulence & pathogenicity traits
- 1. Seminar on Forest Pathology, 29 March 2017 Natural Resources Institute Finland, Viikki, Helsinki Genomic variations in multiple Heterobasidion parviporum isolates: impact on the evolution of virulence trait Zhen Zeng1 , Hui Sun2 , Sebastien Duplessis3 , Eeva J. Vainio4 , Kari Korhonen4 , Fred O. Asiegbu1 1 Department of Forest Sciences, University of Helsinki, Helsinki, Finland, 2 Department of Forest Sciences, Nanjing Forestry University, Nanjing, China. 3 Unité Mixte de Recherche 1136, Institut National de la Recherche Agronomique/Nancy Université, Interactions Arbres/Micro-organismes, Centre de Nancy, 54280 Champenoux, France. 4 Natural Resources Institute Finland, Helsinki, Finland. Email: zhen.zeng@helsinki.fi Fungal genome is very plastic with different types of genomic variations associated with virulence found in different fungal pathogens. Heterobasidion parviporum is the causative agent of root and butt rot disease. The dual lifestyles of this fungus (necrotrophy and saprotrophy) make the mechanism behind its pathogenicity and virulence complicated and remain unsolved. In this project, 15 H. parviporum isolates were collected from forests all across Finland. The virulence test by infecting them on small seedlings of Norway spruce revealed varied virulence among these isolates. Genome-wide alignment and structural variation analysis showed that these isolates shared overall genome structure at macroscopic level, yet have remarkable level of polymorphism in smaller scope. Reads mapping coverage analysis classified genes into 4 groups and genes present exclusively in the most virulent strain may contribute to its virulence. Genes enriched for copy number variations and nucleotide polymorphism are mostly involved in oxidation-reduction and carbohydrate metabolic process and might be relevant to the virulence variations, but higher gene copy cannot guarantee higher virulence. Not a single gene could be pinpointed as a sole determinant for the virulence. Combination with gene expression data during interaction with host in the future would help untangle this complex topic.