Journal club meeting. 1st June 2022

1. Oral Intake of Low-Molecular-Weight Collagen Peptide
Improves Hydration, Elasticity, and Wrinkling in Human
Skin: A Randomized, Double-Blind, Placebo-Controlled
Study
Published: 26th June 2018
Presented by: Dr. Zarka Sarwar , PGT, Physiology

2. • Do-Un Kim1, Hee-Chul Chung1, Jia Choi2, Yasuo Sakai3 and
Boo-Yong Lee2
– 1 Newtree, Seongnam 13207, Gyeonggi, Korea
– 2 Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam 1348
– 3 Central Research Institute, Jellice, Sakae, Tagajo 985-0833, Japan
Citation link:
https://www.researchgate.net/publication/326003109_Oral_Intake_of_Low-Molecular-
Weight_Collagen_Peptide_Improves_Hydration_Elasticity_and_Wrinkling_in_Human_Skin_A_Randomized_Double-
Blind_Placebo-Controlled_Study

4. Outline:
Introduction
Objectives
Methods & Materials
Results
Conclusion
Limitations
Comparison with similar studies
Future recommendations

6. Introduction

7. Introduction
Intrinsic Skin aging Extrinsic Skin Aging
Decline of biological functions + action of Reactive oxygen
species
Chronic sunlight exposure
Not more prominent Skin alterations more prominent
Oxidative stress, nearly inavitable physiological process Solar UV radiation ( other environmental factors)
Gradual process Fast process
Thin skin, Fine wrinkles, dry skin and gradual dermal atrophy Coarse wrinkles, dryness, loss of elasticity, pigmentation
,reduction in the synthesis of hyaluronic acid (HA)
10 % 90%

9. • Collagen, elastic fibers, and HA
• Skin elastic fibers : crosslinked elastin and outer layers of fibril
• The HA content of the dermis is higher than epidermis
• Papillary dermis has greater levels of HA than reticular dermis

10. Major constituents of dermal extracellular matrix
1.Hyluronic acid
2. Collagen
> 70 percent of
skin
3. Elastic fiber

11. • Characteristic of photo-
aged skin
Coarse wrinkles
Loss of elasticity
Dryness and
Pigmentation

12. “SKIN AGING IS INAVITABLE”
• Collagen has been consumed as functional dietary supplements
because of its efficacy for skin health.
• Collagen hydrolysates or collagen peptides (CPs) are also
receiving attention, with studies in vitro and in vivo
investigating the properties of various CPs, including fish-
collagen hydrolysates derived from type I collagen from fish
skin.

13. Preventive measures of skin photo aging
• Required because of inevitable sun exposure
• Exogenously with UV blockage/ ample moisturiser application
• Oral Collagen ( collagen hydrolysate and collagen peptidases)1

14. Hypothesis & Objective
OBJECTIVE:
To investigate the health benefits of LMWCP in human skin,
with a daily oral dosage of 1000 mg of LMWCP for 12 weeks in
64 female volunteers aged 40–60 years and diagnosed as having
photo aged skin.

15. Methods & Materials
• Preparation of Test Material and Determination of Dose

16. Dose calculation
• Thus 1000 mg/day for human was chosen as a dose for human
study from the translated values applied to the average adult
with 60 kg body weight.
(Reagan et al, Dose translation from animal to human studies)

17. Study Design
• Randomized, double-blind, placebo-controlled design. It was
conducted according to the applicable Good Clinical Practice
and the Standard Operating Procedures of Ellead Skin and Bio
Research (Ellead, Seongnam, Gyeonggi-do, Korea) .

19. Inclusion criteria
• Healthy females
• Age 40 to 60
• Diagnosed with crow’s feet (wrinkles)
• Global photo aged score: 2 and 6
• Written/ informed consent
• Cooperative
• Available during study time period
Exclusion criteria
• Hx of allergies
• Diagnosed with any sytemic illness
• Oral/ topical steroids and retinoids used
within 6 months
• Laser or peeling in 3 months
• Participation in similar studies 3 months
• Abnormal LFT’s, other blood tests
• Smokers or Alcoholic
• Pregnancy or breast feeding
• BP > 140/ 90mmhg
• Drug therapy
• Any unsuitable condition judged by
investigator

20. Study Participants
Women aged 40–60 years (n = 70) who volunteered and met specified inclusion and exclusion criteria were recruited
for the study (Table 2). Inclusion criteria included crow’s-feet scores between 2 and 6, as determined by dermatologists
according to the global photodamage scoring system [18]. Before proceeding to the clinical study, the participants
were informed clearly and precisely of the objective and the protocol of the study, and of foreseeable risks involved in
the trial. Participants signed an informed-consent form. Six individuals withdrew consent, and the remaining 64
commenced the study. Eleven participants dropped out of the study for personal reasons, and 53 completed the stud

21. Study Schedule
• Daily Oral intake of one bottle (orally) of their assigned
study
• Refrain from intake of any similar dietary supplements
• Refrain from use of skincare treatments such as face masks
or packs and massages
• Not permitted to apply topical cosmetics except those
provided by Ellead for a 2-week wash-out period before the
study starts and for the 12-week study period, to maintain
constant skin conditions.

22. • At each visit, participants shaved the crow’s-feet area,
• Washed the entire face with foam cleansers,
• Rested for 30 min under the constant relative humidity (40–
60%) and temperature (22–24 °C) prior to assessment.
• The same part of the face was examined at each visit, according
to the same methods used in the examination conducted at 0 W.
Prior to the visit for safety evaluation, participants were
prohibited from eating

24. Measurement of Skin Hydration
Corneometer CM 825 (Courage and Khazaka, Cologne,
Germany).

25. Measurement of Skin Wrinkling
1. Visual Assessment ( crow feet’s area by photo damaged
scoring system)

26. 2. Instrumental Analysis of skin replica Image
(Silicon Based solution and catalyst)

27. Measurement of Skin Elasticity
Cutometer MPA 580 ( Courage and Khazaka)

28. Selection of Test Area
Acetate film face mask for marking of crow's
feet

29. Safety Assessment
Laboratory tests 0Week, 6weeks, 12 weeks
and 12weeks + 2 days
Blood-chemical test Total protein, albumin, aspartate transaminase,
alanine transaminase, γ-GTP, blood urea nitrogen,
creatinine, glucose, total cholesterol, haemoglobin,
hematocrit, white blood cell count, red blood cell
count, platelet count, MCV, MCH and MCHC.
Urine Tests pH, specific gravity, protein, glucose, ketone,
urobilinogen, bilirubin, and nitrite.
Vital-sign measurements Systolic and Diastolic blood pressure and
bodyweight.
Safety of the test material Information collected in interviews and
questionnaires at 6Weeks 12 weeks

30. Statistical Analysis
• Intention To Treat analysis: For safety evaluation
• Per-protocol Analysis: For efficacy evaluation
• Data is Analyzed as descriptive statistics
• Paired t-test: Before and after treatment
• Independent t-test : comparison between the groups

31. Results
• Baseline Characteristics of Participants

32. 1. Effect of LMWCP on Skin Hydration

33. 1.Effect of LMWCP on Skin Hydration

34. 2. Effect of LMWCP on Skin Wrinkling

35. . Effect of LMWCP on Skin Wrinkling

37. Effect of LMWCP on Skin Elasticity

38. Effect of LMWCP on Skin Elasticity

39. Conclusion
• The LMWCP is a safe health functional food ingredient with
anti-skin photoaging efficacy which can effectively improve
hydration, elasticity, and wrinkling in human skin at the dose of
1000 mg once daily.

40. Comparisons with other studies
1.Pyun et al : In hairless mice, oral administration of Low-molecular-
weight Collagen peptide (LMWCP), which is a fish-derived
collagen hydrolysate, promotes recovery of collagen fibers and
normal elastic
2.Shigemura et al: LMWCP may stimulate migration and growth of
fibroblasts thereby exerting favorable effects on skin function,
because dermal fibroblasts enable the synthesis of collagen, elastic
fibers, and dermal HA
3.Ohara et al :The collagen-derived Hyp-containing peptide Pro-Hyp
has been shown to stimulate HA synthesis by activation of HAS2
transcription in human dermal fibroblasts in vitro

41. Strengths of this study
• LMWCP preparation was well tolerated as dietary supplements
• Use of Anti- aging skin agents has been widely practiced
• LMWCP may safe and effective ingredient for other health-
functions
• LMWCP improve skin wrinkling, hydration, and elasticity
markedly
• Readily available
• Affordable

42. limitations
1. Age Group (40-60 yrs)
2. Sample size (60+)
3. Area selected (Face only)
4. Gender (Female)
5. Only Oral LMWCP

43. Future Recommendations
More robust results can be expected in the future studies with
• Large sample size
• Age group above 60 and below 40 years
• Effects on other tissues
• Effects on skin of Other areas of body (hands, Neck and belly)
• Parenteral route administration
• Transdermal administration (fillers)
• Effects on wound healing and scaring
.

46. Thank you.