You have a piece of circular DNA that can be cut by the restriction nucleases EcoRI, HindIII, and NotI, as indicated in the Figure Below... Answer whether the following statement is TRUE or FALSE. If FALSE, explain why. (a) One piece of DNA will be obtained when this DNA is cut by NotI. (b) A piece of DNA that cannot be cut by EcoRI will be obtained by cutting this DNA with both NotI and HindIII. (c) Two DNA fragments that cannot be cut by HindIII will be obtained when this DNA is cut by EcoRI and NotI. (d)Two DNA fragments of unequal size will be created when this DNA is cut by both HindIII and EcoRI. Notl EcoRI EcoRI HindIII Solution 1. True Yes , if the DNA Is cut by only NotI, then only a single piece of DNA would be formed as there would be a cut in the circular DNA , thus forming a single piece single stranded DNA. 2. False Even if there is a cut with both NotI and HindIII there would be a piece of DNA between NotI and ECoRI which will still remain and won\'t be cut. 3. True Two DNA fragments will be obtained when cut with EcoRI and NotI that cannot be obtained with HindIII. A fragment between NotI and ECoRI and between the two ECoRI. 4. False Three unequal pieces of DNA would be formed. First, the HindIII, would cause a nick and the strand would open and form a single strand. Second, ECoRI would cause two nicks, as it is at two places, thus two more fragments, one between the two ECoRI and the other between the ECoRI and HindIII would be formed..

1. You have a piece of circular DNA that can be cut by the restriction nucleases EcoRI, HindIII,
and NotI, as indicated in the Figure Below...
Answer whether the following statement is TRUE or FALSE. If FALSE, explain why.
(a) One piece of DNA will be obtained when this DNA is cut by NotI.
(b) A piece of DNA that cannot be cut by EcoRI will be obtained by cutting this DNA with both
NotI and HindIII.
(c) Two DNA fragments that cannot be cut by HindIII will be obtained when this DNA is cut by
EcoRI and NotI.
(d)Two DNA fragments of unequal size will be created when this DNA is cut by both HindIII
and EcoRI. Notl EcoRI EcoRI HindIII
Solution
1. True
Yes , if the DNA Is cut by only NotI, then only a single piece of DNA would be formed as there
would be a cut in the circular DNA , thus forming a single piece single stranded DNA.
2. False
Even if there is a cut with both NotI and HindIII there would be a piece of DNA between NotI
and ECoRI which will still remain and won't be cut.
3. True
Two DNA fragments will be obtained when cut with EcoRI and NotI that cannot be obtained
with HindIII. A fragment between NotI and ECoRI and between the two ECoRI.
4. False
Three unequal pieces of DNA would be formed. First, the HindIII, would cause a nick and the
strand would open and form a single strand. Second, ECoRI would cause two nicks, as it is at
two places, thus two more fragments, one between the two ECoRI and the other between the
ECoRI and HindIII would be formed.