You are asked to determine DNA content of a liver tissue. Describe how you will porceed in finding amount of DNA in the sample Take a piece of live tissue and subject it to detergent solution i.e. SDS (sodiumdodecyl sulfate) so that the cell membrane of the cells will be removed out through micelle formation & then alcohol is added as DNA soluble in water. This is followed by the formation of aqueous phase with DNA, precipitate layer with protein and bottom organic layer. Now the purity of DNA sample is calculated to know the “total amount of DNA in the sample as ng DNA / µg dry weight”. There are various processes applicable to determine total DNA content using either cloning, qPCR, sequencing. However, initial purity of DNA can be calculated by measuring absorbance or OD260/OD280 ratio at 280nm using “spectrophotomemeter” (UV-Visible). The range of OD with absorption of UV light between 1.8 to 2.0 denotes the presence of DNA. Lower than this OD ratio, denotes the presence of proteins. The another method of examining the sample is through & this method is useful to deterimine total DNA concentration by use of the fluorochrome Hoechst 33258 & filters, & scanning fluorescence spectrophotometer at emission at 458 nm. Solution You are asked to determine DNA content of a liver tissue. Describe how you will porceed in finding amount of DNA in the sample Take a piece of live tissue and subject it to detergent solution i.e. SDS (sodiumdodecyl sulfate) so that the cell membrane of the cells will be removed out through micelle formation & then alcohol is added as DNA soluble in water. This is followed by the formation of aqueous phase with DNA, precipitate layer with protein and bottom organic layer. Now the purity of DNA sample is calculated to know the “total amount of DNA in the sample as ng DNA / µg dry weight”. There are various processes applicable to determine total DNA content using either cloning, qPCR, sequencing. However, initial purity of DNA can be calculated by measuring absorbance or OD260/OD280 ratio at 280nm using “spectrophotomemeter” (UV-Visible). The range of OD with absorption of UV light between 1.8 to 2.0 denotes the presence of DNA. Lower than this OD ratio, denotes the presence of proteins. The another method of examining the sample is through & this method is useful to deterimine total DNA concentration by use of the fluorochrome Hoechst 33258 & filters, & scanning fluorescence spectrophotometer at emission at 458 nm..

1. You are asked to determine DNA content of a liver tissue. Describe how you will porceed in
finding amount of DNA in the sample
Take a piece of live tissue and subject it to detergent solution i.e. SDS (sodiumdodecyl sulfate)
so that the cell membrane of the cells will be removed out through micelle formation & then
alcohol is added as DNA soluble in water. This is followed by the formation of aqueous phase
with DNA, precipitate layer with protein and bottom organic layer. Now the purity of DNA
sample is calculated to know the “total amount of DNA in the sample as ng DNA / µg dry
weight”. There are various processes applicable to determine total DNA content using either
cloning, qPCR, sequencing. However, initial purity of DNA can be calculated by measuring
absorbance or OD260/OD280 ratio at 280nm using “spectrophotomemeter” (UV-Visible). The
range of OD with absorption of UV light between 1.8 to 2.0 denotes the presence of DNA.
Lower than this OD ratio, denotes the presence of proteins. The another method of examining the
sample is through & this method is useful to deterimine total DNA concentration by use of the
fluorochrome Hoechst 33258 & filters, & scanning fluorescence spectrophotometer at emission
at 458 nm.
Solution
You are asked to determine DNA content of a liver tissue. Describe how you will porceed in
finding amount of DNA in the sample
Take a piece of live tissue and subject it to detergent solution i.e. SDS (sodiumdodecyl sulfate)
so that the cell membrane of the cells will be removed out through micelle formation & then
alcohol is added as DNA soluble in water. This is followed by the formation of aqueous phase
with DNA, precipitate layer with protein and bottom organic layer. Now the purity of DNA
sample is calculated to know the “total amount of DNA in the sample as ng DNA / µg dry
weight”. There are various processes applicable to determine total DNA content using either
cloning, qPCR, sequencing. However, initial purity of DNA can be calculated by measuring
absorbance or OD260/OD280 ratio at 280nm using “spectrophotomemeter” (UV-Visible). The
range of OD with absorption of UV light between 1.8 to 2.0 denotes the presence of DNA.
Lower than this OD ratio, denotes the presence of proteins. The another method of examining the
sample is through & this method is useful to deterimine total DNA concentration by use of the
fluorochrome Hoechst 33258 & filters, & scanning fluorescence spectrophotometer at emission
at 458 nm.