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Is Yeast
Alive?
How to Set Up the
“Is Yeast Alive?”
Lab
4 Test Tubes
Test Tube Rack
Graduated
Cylinder
Sugar (2 packs)
Yeast (4 cups)
Marker
Stickers
4 Balloons
Beaker
Materials and Equipment
Step 1:
Set up four test tubes in a test tube rack.
4 Test Tubes
Test Tube Rack
Step 2:
Place a sticker on each test tube.
Test Tube
Sticker
Step 3:
Label each test tube with a number (1 - 4).
Test Tube
Sticker
Label with number
Measure 10 mL of
warm water from
beaker using
graduated cylinder.
Beaker
Graduated cylinder
BeakerBeaker
Graduated cylinder
Beaker
Graduated cylinder
Beaker
Step 4:
10 mL
Remember, read the
bottom of the
meniscus (curved
upper surface of a
liquid in a tube) to find
the volume.
Step 4:
(cont)
Add the 10 mL of warm water to
each of the 4 labeled test tubes!
Labeled
Test Tube
Graduated
cylinder with
10 mL water
Step 5:
Add one mini-cup of dry
yeast to each of the four test
tubes. (1 cup = 1 test tube)
Test Tube
Cups of yeast
Step 6:
Sugar Packet
Test Tube 1 Test Tube 2
Add one packet of sugar to test tube 1
and one packet of sugar to test tube 2.
Do NOT add sugar to test tube 3 or test
tube 4!
Step 7:
With 2 people…
With 1 person…
Finished…
Cover the opening of each test
tube with a balloon to trap any gas.
This can either be done by one or
two people. (see examples above)
Step 8:
Your thumb...
Hold your thumb over
the end of each test
tube and shake it
vigorously until the
contents are completely
mixed.
Shake
Shake
Step 9
Step 10: Remember to
write down all
observations and
make detailed
sketches in your
Science Journal!
Return test tubes to the rack and record initial observations in
your Science Journal.
Then, every 5 minutes observe and record any changes.
Include additional sketches and written observations.
Science Journal!
Congratulations.
You have successfully set up our
“Is Yeast Alive?” Lab.
Please be sure to include detailed
sketches and written observations
in your Science Journal.
Don’t forget to record any errors
or mistakes your encounter
during your lab.

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Yeast instructions

  • 1. Is Yeast Alive? How to Set Up the “Is Yeast Alive?” Lab
  • 2. 4 Test Tubes Test Tube Rack Graduated Cylinder Sugar (2 packs) Yeast (4 cups) Marker Stickers 4 Balloons Beaker Materials and Equipment
  • 3. Step 1: Set up four test tubes in a test tube rack. 4 Test Tubes Test Tube Rack
  • 4. Step 2: Place a sticker on each test tube. Test Tube Sticker
  • 5. Step 3: Label each test tube with a number (1 - 4). Test Tube Sticker Label with number
  • 6. Measure 10 mL of warm water from beaker using graduated cylinder. Beaker Graduated cylinder BeakerBeaker Graduated cylinder Beaker Graduated cylinder Beaker Step 4: 10 mL Remember, read the bottom of the meniscus (curved upper surface of a liquid in a tube) to find the volume.
  • 7. Step 4: (cont) Add the 10 mL of warm water to each of the 4 labeled test tubes! Labeled Test Tube Graduated cylinder with 10 mL water
  • 8. Step 5: Add one mini-cup of dry yeast to each of the four test tubes. (1 cup = 1 test tube) Test Tube Cups of yeast
  • 9. Step 6: Sugar Packet Test Tube 1 Test Tube 2 Add one packet of sugar to test tube 1 and one packet of sugar to test tube 2. Do NOT add sugar to test tube 3 or test tube 4!
  • 10. Step 7: With 2 people… With 1 person… Finished… Cover the opening of each test tube with a balloon to trap any gas. This can either be done by one or two people. (see examples above)
  • 11. Step 8: Your thumb... Hold your thumb over the end of each test tube and shake it vigorously until the contents are completely mixed. Shake Shake
  • 12. Step 9 Step 10: Remember to write down all observations and make detailed sketches in your Science Journal! Return test tubes to the rack and record initial observations in your Science Journal. Then, every 5 minutes observe and record any changes. Include additional sketches and written observations. Science Journal!
  • 13. Congratulations. You have successfully set up our “Is Yeast Alive?” Lab. Please be sure to include detailed sketches and written observations in your Science Journal. Don’t forget to record any errors or mistakes your encounter during your lab.