1. This study used 1H-NMR spectroscopy to investigate the transportation of monocrotaline (MCT), a toxic pyrrolizidine alkaloid, and its metabolites dehydromonocrotaline (DHM) and dehydroretronecine (DHR) in human blood plasma and red blood cells (RBCs). 2. The study found that MCT and its precursor retronecine (RET) remained intact in plasma and could enter RBCs. DHM rapidly hydrolyzed in plasma but bound to RBC contents. DHR disappeared immediately in plasma. 3. The results confirm that both plasma and RBCs can transport MCT