SlideShare a Scribd company logo
Evodiamine isolated from Evodia rutaecarpa as human
topoisomerase catalytic inhibitor
Xiaobei Pan1
, Janet M. Hartley2
, John A. Hartley2
, Kenneth N White1
,
Zhengtao Wang1,3
, S. W. Annie Bligh1
1
Institute for Health Research and Policy, London Metropolitan University, 166-220 Holloway Road, London N7 8DB, UK
2
Cancer Research UK Drug-DNA Interactions Research Group, UCL Cancer Institute, University College London, Paul O’Gorman Building, London, UK
3
The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese
Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Zhangjiang Hi-tech Park, Shanghai 201210, China
Introduction
 Topoisomerase (Fig. 1) is an enzyme, which
can relieve torsional stress in DNA.
 Topoisomerase I and II cleave the single
and double stranded DNA, respectively.
 Since camptothecin was found as a
topoisomerase inhibitor, a number of semi-
synthetic compounds were successfully
developed and used to treat cancer.
 Evodia rutaecarpa (Fig. 2) is a Chinese
herbal medicinal plant that has been used to
treat gastrointestinal disorders, bacterial
infection & inflammation-related disorders.
 Evodiamine isolated from Evodia rutaecarpa
is cytotoxic to several cancer cell lines.
Aims and Objectives
 To isolate quinoline alkaloids from the Evodia and structure elucidation
 To study their potential cytotoxicity, inhibitory activity of topoisomerase I and
II, and catalytic mechanisms.
Methods
 Alkaloids were isolated using reverse-phase HPLC and the structures were
elucidated by 1-D, 2-D NMR spectroscopy and mass spectrometry.
 Their cytotoxicities were determined using MTT-based assay.
 Topoisomerase (Topo) inhibitory activities were studied using Topo I relaxation
and Topo II decatanation assay.
 Comet assay and cell cycle distribution study were carried out by flow
cytometry.
Results and Discussion
 Eight alkaloids were isolated from Evodia, of which evodiamine (Fig. 3)
showed the most potent activity against human leukemia K562 and THP-1
cell lines (IC50 34.35 µM and 90.87 µM respectively.)
 Evodiamine is an inhibitor for both human Topo I and Topo II (Fig. 4).
 It did not induce DNA strand breaks at the cellular level (Fig. 5) and
arrested cell growth at the G2/M phase in the cell cycle.
N
H
N
N
O2
3
5
6
78
9
10
11
12
13
15
16
17
18
19
20
21
H
H3C
Conclusion
Evodiamine was found to be a dual inhibitor for both topoisomerase I and II
with potent cytotoxicity against human leukemia cell lines.
Figure 1. Structure of topoisomerase
Figure 2. Evodia rutaecarpa
Figure 3. Structure of Evodiamine
Figure 4. IC50 of evodiamine against human Topo I
(A, C) using relaxation assay and Topo II (B, D)
using decatanation assayFigure 5. The comet assay did not detect DNA
strand breaks induced by evodiamine
Acknowledgement
X.Pan thanks the Department of Human Science at London Metropolitan University for a student bursary.

More Related Content

What's hot

Effects of hypoxia on human cancer cell line chemosensitivity
Effects of hypoxia on human cancer cell line chemosensitivityEffects of hypoxia on human cancer cell line chemosensitivity
Effects of hypoxia on human cancer cell line chemosensitivity
Enrique Moreno Gonzalez
 
Protective role of co q10 or l carnitine on the integrity of the myocardium i...
Protective role of co q10 or l carnitine on the integrity of the myocardium i...Protective role of co q10 or l carnitine on the integrity of the myocardium i...
Protective role of co q10 or l carnitine on the integrity of the myocardium i...
Prof. Hesham N. Mustafa
 
Murdannia loriformis(hassk) - Yapakking
Murdannia loriformis(hassk) - Yapakking Murdannia loriformis(hassk) - Yapakking
Murdannia loriformis(hassk) - Yapakking
Suphalada C.
 
Environmental exposure and health risks of the insecticide monocrotophos - a ...
Environmental exposure and health risks of the insecticide monocrotophos - a ...Environmental exposure and health risks of the insecticide monocrotophos - a ...
Environmental exposure and health risks of the insecticide monocrotophos - a ...
Innspub Net
 
Amiji poster
Amiji posterAmiji poster
Amiji poster
EdwardPham9
 
CHROMB17026[1]
CHROMB17026[1]CHROMB17026[1]
CHROMB17026[1]
Deqing Xiao
 
2
22
Comprehensive assessment of chamber-specific and transmural heterogeneity in ...
Comprehensive assessment of chamber-specific and transmural heterogeneity in ...Comprehensive assessment of chamber-specific and transmural heterogeneity in ...
Comprehensive assessment of chamber-specific and transmural heterogeneity in ...
Justin Doop
 
Axl inhibitor (1)
Axl inhibitor (1)Axl inhibitor (1)
Axl inhibitor (1)
VankteshKumar
 
Non biopsy diagnosis of acute rejection of Renal allograft
  Non biopsy diagnosis of acute rejection of Renal allograft  Non biopsy diagnosis of acute rejection of Renal allograft
Non biopsy diagnosis of acute rejection of Renal allograft
Bakshish Singh
 
Pharmacogenetics - Pharmacokinetics
Pharmacogenetics  - Pharmacokinetics Pharmacogenetics  - Pharmacokinetics
Pharmacogenetics - Pharmacokinetics
Areej Abu Hanieh
 
Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...
Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...
Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...
College of Pharmacy,Sri Ramakrishna Institute of Paramedical Sciences,Coimbatore
 
M. Celeste Simón-Homenaje al bioquímico español Alberto Sols
M. Celeste Simón-Homenaje al bioquímico español Alberto SolsM. Celeste Simón-Homenaje al bioquímico español Alberto Sols
M. Celeste Simón-Homenaje al bioquímico español Alberto Sols
Fundación Ramón Areces
 
Genetic polymorphism in drug transport
Genetic polymorphism in drug transportGenetic polymorphism in drug transport
Genetic polymorphism in drug transport
Vineetha Menon
 
In vitro metabolite profiling
In vitro metabolite profilingIn vitro metabolite profiling
In vitro metabolite profiling
Tejaswini Jadhav
 
2012-JCI
2012-JCI2012-JCI
2012-JCI
Wei-Chih Tsai
 
Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...
Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...
Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...
Mark Lipstein
 
ASMSPoster_2015
ASMSPoster_2015ASMSPoster_2015
ASMSPoster_2015
Jonathan Oyler
 
Pharmacology bao jiaolin
Pharmacology bao jiaolinPharmacology bao jiaolin
Pharmacology bao jiaolin
Jiaolin Bao
 

What's hot (19)

Effects of hypoxia on human cancer cell line chemosensitivity
Effects of hypoxia on human cancer cell line chemosensitivityEffects of hypoxia on human cancer cell line chemosensitivity
Effects of hypoxia on human cancer cell line chemosensitivity
 
Protective role of co q10 or l carnitine on the integrity of the myocardium i...
Protective role of co q10 or l carnitine on the integrity of the myocardium i...Protective role of co q10 or l carnitine on the integrity of the myocardium i...
Protective role of co q10 or l carnitine on the integrity of the myocardium i...
 
Murdannia loriformis(hassk) - Yapakking
Murdannia loriformis(hassk) - Yapakking Murdannia loriformis(hassk) - Yapakking
Murdannia loriformis(hassk) - Yapakking
 
Environmental exposure and health risks of the insecticide monocrotophos - a ...
Environmental exposure and health risks of the insecticide monocrotophos - a ...Environmental exposure and health risks of the insecticide monocrotophos - a ...
Environmental exposure and health risks of the insecticide monocrotophos - a ...
 
Amiji poster
Amiji posterAmiji poster
Amiji poster
 
CHROMB17026[1]
CHROMB17026[1]CHROMB17026[1]
CHROMB17026[1]
 
2
22
2
 
Comprehensive assessment of chamber-specific and transmural heterogeneity in ...
Comprehensive assessment of chamber-specific and transmural heterogeneity in ...Comprehensive assessment of chamber-specific and transmural heterogeneity in ...
Comprehensive assessment of chamber-specific and transmural heterogeneity in ...
 
Axl inhibitor (1)
Axl inhibitor (1)Axl inhibitor (1)
Axl inhibitor (1)
 
Non biopsy diagnosis of acute rejection of Renal allograft
  Non biopsy diagnosis of acute rejection of Renal allograft  Non biopsy diagnosis of acute rejection of Renal allograft
Non biopsy diagnosis of acute rejection of Renal allograft
 
Pharmacogenetics - Pharmacokinetics
Pharmacogenetics  - Pharmacokinetics Pharmacogenetics  - Pharmacokinetics
Pharmacogenetics - Pharmacokinetics
 
Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...
Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...
Synthesis, Docking Studies and Anticancer Activity of New Substituted Pyrimid...
 
M. Celeste Simón-Homenaje al bioquímico español Alberto Sols
M. Celeste Simón-Homenaje al bioquímico español Alberto SolsM. Celeste Simón-Homenaje al bioquímico español Alberto Sols
M. Celeste Simón-Homenaje al bioquímico español Alberto Sols
 
Genetic polymorphism in drug transport
Genetic polymorphism in drug transportGenetic polymorphism in drug transport
Genetic polymorphism in drug transport
 
In vitro metabolite profiling
In vitro metabolite profilingIn vitro metabolite profiling
In vitro metabolite profiling
 
2012-JCI
2012-JCI2012-JCI
2012-JCI
 
Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...
Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...
Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kd...
 
ASMSPoster_2015
ASMSPoster_2015ASMSPoster_2015
ASMSPoster_2015
 
Pharmacology bao jiaolin
Pharmacology bao jiaolinPharmacology bao jiaolin
Pharmacology bao jiaolin
 

Viewers also liked

Källkritik20101118
Källkritik20101118Källkritik20101118
Källkritik20101118
Kristina Alexanderson
 
Wordpress: Tio tips
Wordpress: Tio tipsWordpress: Tio tips
Wordpress: Tio tipsitmamman
 
Marita Isaksson JOINA
Marita Isaksson JOINAMarita Isaksson JOINA
Projekt bezpečně v kyberprostoru
Projekt bezpečně v kyberprostoru Projekt bezpečně v kyberprostoru
Projekt bezpečně v kyberprostoru
Pohanka
 
Druk 3D - where is the money? Michał Frączek – Materialination
Druk 3D - where is the money? Michał Frączek – MaterialinationDruk 3D - where is the money? Michał Frączek – Materialination
Druk 3D - where is the money? Michał Frączek – Materialination
Fundacja Rozwoju Branży Internetowej Netcamp
 
Early Magazine Article on "How-to-Choose an Internet Service Provider"
Early Magazine Article on "How-to-Choose an Internet Service Provider"Early Magazine Article on "How-to-Choose an Internet Service Provider"
Early Magazine Article on "How-to-Choose an Internet Service Provider"
cvanorden
 

Viewers also liked (7)

Källkritik20101118
Källkritik20101118Källkritik20101118
Källkritik20101118
 
Wordpress: Tio tips
Wordpress: Tio tipsWordpress: Tio tips
Wordpress: Tio tips
 
Marita Isaksson JOINA
Marita Isaksson JOINAMarita Isaksson JOINA
Marita Isaksson JOINA
 
Projekt bezpečně v kyberprostoru
Projekt bezpečně v kyberprostoru Projekt bezpečně v kyberprostoru
Projekt bezpečně v kyberprostoru
 
Session 6 Lars Westin
Session 6 Lars WestinSession 6 Lars Westin
Session 6 Lars Westin
 
Druk 3D - where is the money? Michał Frączek – Materialination
Druk 3D - where is the money? Michał Frączek – MaterialinationDruk 3D - where is the money? Michał Frączek – Materialination
Druk 3D - where is the money? Michał Frączek – Materialination
 
Early Magazine Article on "How-to-Choose an Internet Service Provider"
Early Magazine Article on "How-to-Choose an Internet Service Provider"Early Magazine Article on "How-to-Choose an Internet Service Provider"
Early Magazine Article on "How-to-Choose an Internet Service Provider"
 

Similar to Pan xiao

Cm ecs2016
Cm ecs2016Cm ecs2016
Cm ecs2016
Chrysanthi Matheou
 
Molecular Mechanisms of Radiation Damage.
Molecular Mechanisms of Radiation Damage. Molecular Mechanisms of Radiation Damage.
Molecular Mechanisms of Radiation Damage.
Dmitri Popov
 
NMT_JMedChem2
NMT_JMedChem2NMT_JMedChem2
NMT_JMedChem2
Stephen Brand
 
Medical review of medolife’s escozine tm technology in pre clinical & cli...
Medical review of medolife’s escozine tm technology in pre clinical & cli...Medical review of medolife’s escozine tm technology in pre clinical & cli...
Medical review of medolife’s escozine tm technology in pre clinical & cli...
PetLife Pharmaceuticals Inc
 
Thesis defense - QUANG ONG - FINAL
Thesis defense - QUANG ONG - FINALThesis defense - QUANG ONG - FINAL
Thesis defense - QUANG ONG - FINAL
Quang Ong
 
mitochondria poster
mitochondria postermitochondria poster
mitochondria poster
Abhinav Suri
 
ZechSG16-acs-jmedchem-5b01552
ZechSG16-acs-jmedchem-5b01552ZechSG16-acs-jmedchem-5b01552
ZechSG16-acs-jmedchem-5b01552
Stephan G. Zech
 
HepG2 cell model for genotoxicity and steatosis assessment
HepG2 cell model for genotoxicity and steatosis assessmentHepG2 cell model for genotoxicity and steatosis assessment
HepG2 cell model for genotoxicity and steatosis assessment
HCS Pharma
 
Drug induced hepatotoxicity and its regulatory implications
Drug induced hepatotoxicity and its regulatory implicationsDrug induced hepatotoxicity and its regulatory implications
Drug induced hepatotoxicity and its regulatory implications
Chander K Negi
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
daranisaha
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
semualkaira
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
semualkaira
 
Assignment on Toxicology
Assignment on ToxicologyAssignment on Toxicology
Assignment on Toxicology
Deepak Kumar
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
AnonIshanvi
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
NainaAnon
 
UROP poster
UROP posterUROP poster
NMT_Nature
NMT_NatureNMT_Nature
NMT_Nature
Stephen Brand
 
Metallothionein 2A expression and its relation to different clinical stages
Metallothionein 2A expression and its relation to different clinical stagesMetallothionein 2A expression and its relation to different clinical stages
Metallothionein 2A expression and its relation to different clinical stages
wael mansy
 
Effects Of Oxygen On Radiation Therapy
Effects Of  Oxygen On  Radiation  TherapyEffects Of  Oxygen On  Radiation  Therapy
Effects Of Oxygen On Radiation Therapy
fondas vakalis
 
Omics technology: an important tool in mechanism studies of Chinese herbal fo...
Omics technology: an important tool in mechanism studies of Chinese herbal fo...Omics technology: an important tool in mechanism studies of Chinese herbal fo...
Omics technology: an important tool in mechanism studies of Chinese herbal fo...
LucyPi1
 

Similar to Pan xiao (20)

Cm ecs2016
Cm ecs2016Cm ecs2016
Cm ecs2016
 
Molecular Mechanisms of Radiation Damage.
Molecular Mechanisms of Radiation Damage. Molecular Mechanisms of Radiation Damage.
Molecular Mechanisms of Radiation Damage.
 
NMT_JMedChem2
NMT_JMedChem2NMT_JMedChem2
NMT_JMedChem2
 
Medical review of medolife’s escozine tm technology in pre clinical & cli...
Medical review of medolife’s escozine tm technology in pre clinical & cli...Medical review of medolife’s escozine tm technology in pre clinical & cli...
Medical review of medolife’s escozine tm technology in pre clinical & cli...
 
Thesis defense - QUANG ONG - FINAL
Thesis defense - QUANG ONG - FINALThesis defense - QUANG ONG - FINAL
Thesis defense - QUANG ONG - FINAL
 
mitochondria poster
mitochondria postermitochondria poster
mitochondria poster
 
ZechSG16-acs-jmedchem-5b01552
ZechSG16-acs-jmedchem-5b01552ZechSG16-acs-jmedchem-5b01552
ZechSG16-acs-jmedchem-5b01552
 
HepG2 cell model for genotoxicity and steatosis assessment
HepG2 cell model for genotoxicity and steatosis assessmentHepG2 cell model for genotoxicity and steatosis assessment
HepG2 cell model for genotoxicity and steatosis assessment
 
Drug induced hepatotoxicity and its regulatory implications
Drug induced hepatotoxicity and its regulatory implicationsDrug induced hepatotoxicity and its regulatory implications
Drug induced hepatotoxicity and its regulatory implications
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
 
Assignment on Toxicology
Assignment on ToxicologyAssignment on Toxicology
Assignment on Toxicology
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
 
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
A 43-Year-Old Male with PCM1-JAK2 Gene Fusion Experienced T-Lymphoblastic Lym...
 
UROP poster
UROP posterUROP poster
UROP poster
 
NMT_Nature
NMT_NatureNMT_Nature
NMT_Nature
 
Metallothionein 2A expression and its relation to different clinical stages
Metallothionein 2A expression and its relation to different clinical stagesMetallothionein 2A expression and its relation to different clinical stages
Metallothionein 2A expression and its relation to different clinical stages
 
Effects Of Oxygen On Radiation Therapy
Effects Of  Oxygen On  Radiation  TherapyEffects Of  Oxygen On  Radiation  Therapy
Effects Of Oxygen On Radiation Therapy
 
Omics technology: an important tool in mechanism studies of Chinese herbal fo...
Omics technology: an important tool in mechanism studies of Chinese herbal fo...Omics technology: an important tool in mechanism studies of Chinese herbal fo...
Omics technology: an important tool in mechanism studies of Chinese herbal fo...
 

More from LondonMet PGR Students

Inflation targeting misfiring on development of housing market bubble
Inflation targeting misfiring on development of housing market bubbleInflation targeting misfiring on development of housing market bubble
Inflation targeting misfiring on development of housing market bubble
LondonMet PGR Students
 
Md kanu
Md kanuMd kanu
Jabeen shah
Jabeen shahJabeen shah
Glyn robbins
Glyn robbinsGlyn robbins
Robert gonouya
Robert gonouyaRobert gonouya
Robert gonouya
LondonMet PGR Students
 
Janet bowstead poster
Janet bowstead posterJanet bowstead poster
Janet bowstead poster
LondonMet PGR Students
 
Veronica azolukwam
Veronica azolukwamVeronica azolukwam
Veronica azolukwam
LondonMet PGR Students
 
Deepthi ratnayake
Deepthi ratnayakeDeepthi ratnayake
Deepthi ratnayake
LondonMet PGR Students
 
Veronica azolukwam
Veronica azolukwamVeronica azolukwam
Veronica azolukwam
LondonMet PGR Students
 
Marta kaleta
Marta kaletaMarta kaleta
Shazaib butt
Shazaib buttShazaib butt
Ozlem edizel
Ozlem edizelOzlem edizel
Thao nguyen
Thao nguyenThao nguyen
Qicheng yu
Qicheng yuQicheng yu
Mohammad khaleq newaz
Mohammad khaleq newazMohammad khaleq newaz
Mohammad khaleq newaz
LondonMet PGR Students
 
Joycelyn adinkrah
Joycelyn adinkrahJoycelyn adinkrah
Joycelyn adinkrah
LondonMet PGR Students
 
Janet bowstead
Janet bowsteadJanet bowstead
Janet bowstead
LondonMet PGR Students
 
Syahnaz mohdmokhter
Syahnaz mohdmokhterSyahnaz mohdmokhter
Syahnaz mohdmokhter
LondonMet PGR Students
 

More from LondonMet PGR Students (20)

Sara cannizzaro
Sara cannizzaroSara cannizzaro
Sara cannizzaro
 
Inflation targeting misfiring on development of housing market bubble
Inflation targeting misfiring on development of housing market bubbleInflation targeting misfiring on development of housing market bubble
Inflation targeting misfiring on development of housing market bubble
 
Md kanu
Md kanuMd kanu
Md kanu
 
Jabeen shah
Jabeen shahJabeen shah
Jabeen shah
 
Glyn robbins
Glyn robbinsGlyn robbins
Glyn robbins
 
Robert gonouya
Robert gonouyaRobert gonouya
Robert gonouya
 
Janet bowstead poster
Janet bowstead posterJanet bowstead poster
Janet bowstead poster
 
Veronica azolukwam
Veronica azolukwamVeronica azolukwam
Veronica azolukwam
 
Deepthi ratnayake
Deepthi ratnayakeDeepthi ratnayake
Deepthi ratnayake
 
Veronica azolukwam
Veronica azolukwamVeronica azolukwam
Veronica azolukwam
 
Marta kaleta
Marta kaletaMarta kaleta
Marta kaleta
 
Tracy part
Tracy partTracy part
Tracy part
 
Shazaib butt
Shazaib buttShazaib butt
Shazaib butt
 
Ozlem edizel
Ozlem edizelOzlem edizel
Ozlem edizel
 
Thao nguyen
Thao nguyenThao nguyen
Thao nguyen
 
Qicheng yu
Qicheng yuQicheng yu
Qicheng yu
 
Mohammad khaleq newaz
Mohammad khaleq newazMohammad khaleq newaz
Mohammad khaleq newaz
 
Joycelyn adinkrah
Joycelyn adinkrahJoycelyn adinkrah
Joycelyn adinkrah
 
Janet bowstead
Janet bowsteadJanet bowstead
Janet bowstead
 
Syahnaz mohdmokhter
Syahnaz mohdmokhterSyahnaz mohdmokhter
Syahnaz mohdmokhter
 

Recently uploaded

GraphRAG for Life Science to increase LLM accuracy
GraphRAG for Life Science to increase LLM accuracyGraphRAG for Life Science to increase LLM accuracy
GraphRAG for Life Science to increase LLM accuracy
Tomaz Bratanic
 
Pushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 daysPushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 days
Adtran
 
Serial Arm Control in Real Time Presentation
Serial Arm Control in Real Time PresentationSerial Arm Control in Real Time Presentation
Serial Arm Control in Real Time Presentation
tolgahangng
 
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
SOFTTECHHUB
 
Communications Mining Series - Zero to Hero - Session 1
Communications Mining Series - Zero to Hero - Session 1Communications Mining Series - Zero to Hero - Session 1
Communications Mining Series - Zero to Hero - Session 1
DianaGray10
 
Microsoft - Power Platform_G.Aspiotis.pdf
Microsoft - Power Platform_G.Aspiotis.pdfMicrosoft - Power Platform_G.Aspiotis.pdf
Microsoft - Power Platform_G.Aspiotis.pdf
Uni Systems S.M.S.A.
 
Artificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopmentArtificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopment
Octavian Nadolu
 
UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5
DianaGray10
 
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
AI 101: An Introduction to the Basics and Impact of Artificial IntelligenceAI 101: An Introduction to the Basics and Impact of Artificial Intelligence
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
IndexBug
 
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERPBest 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
Pixlogix Infotech
 
RESUME BUILDER APPLICATION Project for students
RESUME BUILDER APPLICATION Project for studentsRESUME BUILDER APPLICATION Project for students
RESUME BUILDER APPLICATION Project for students
KAMESHS29
 
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAUHCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
panagenda
 
How to use Firebase Data Connect For Flutter
How to use Firebase Data Connect For FlutterHow to use Firebase Data Connect For Flutter
How to use Firebase Data Connect For Flutter
Daiki Mogmet Ito
 
“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”
Claudio Di Ciccio
 
How to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptxHow to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptx
danishmna97
 
Introduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - CybersecurityIntroduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - Cybersecurity
mikeeftimakis1
 
UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6
DianaGray10
 
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc
 
20240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 202420240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 2024
Matthew Sinclair
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
shyamraj55
 

Recently uploaded (20)

GraphRAG for Life Science to increase LLM accuracy
GraphRAG for Life Science to increase LLM accuracyGraphRAG for Life Science to increase LLM accuracy
GraphRAG for Life Science to increase LLM accuracy
 
Pushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 daysPushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 days
 
Serial Arm Control in Real Time Presentation
Serial Arm Control in Real Time PresentationSerial Arm Control in Real Time Presentation
Serial Arm Control in Real Time Presentation
 
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
 
Communications Mining Series - Zero to Hero - Session 1
Communications Mining Series - Zero to Hero - Session 1Communications Mining Series - Zero to Hero - Session 1
Communications Mining Series - Zero to Hero - Session 1
 
Microsoft - Power Platform_G.Aspiotis.pdf
Microsoft - Power Platform_G.Aspiotis.pdfMicrosoft - Power Platform_G.Aspiotis.pdf
Microsoft - Power Platform_G.Aspiotis.pdf
 
Artificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopmentArtificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopment
 
UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5
 
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
AI 101: An Introduction to the Basics and Impact of Artificial IntelligenceAI 101: An Introduction to the Basics and Impact of Artificial Intelligence
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
 
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERPBest 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
 
RESUME BUILDER APPLICATION Project for students
RESUME BUILDER APPLICATION Project for studentsRESUME BUILDER APPLICATION Project for students
RESUME BUILDER APPLICATION Project for students
 
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAUHCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
 
How to use Firebase Data Connect For Flutter
How to use Firebase Data Connect For FlutterHow to use Firebase Data Connect For Flutter
How to use Firebase Data Connect For Flutter
 
“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”
 
How to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptxHow to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptx
 
Introduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - CybersecurityIntroduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - Cybersecurity
 
UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6
 
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy Survey
 
20240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 202420240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 2024
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
 

Pan xiao

  • 1. Evodiamine isolated from Evodia rutaecarpa as human topoisomerase catalytic inhibitor Xiaobei Pan1 , Janet M. Hartley2 , John A. Hartley2 , Kenneth N White1 , Zhengtao Wang1,3 , S. W. Annie Bligh1 1 Institute for Health Research and Policy, London Metropolitan University, 166-220 Holloway Road, London N7 8DB, UK 2 Cancer Research UK Drug-DNA Interactions Research Group, UCL Cancer Institute, University College London, Paul O’Gorman Building, London, UK 3 The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Zhangjiang Hi-tech Park, Shanghai 201210, China Introduction  Topoisomerase (Fig. 1) is an enzyme, which can relieve torsional stress in DNA.  Topoisomerase I and II cleave the single and double stranded DNA, respectively.  Since camptothecin was found as a topoisomerase inhibitor, a number of semi- synthetic compounds were successfully developed and used to treat cancer.  Evodia rutaecarpa (Fig. 2) is a Chinese herbal medicinal plant that has been used to treat gastrointestinal disorders, bacterial infection & inflammation-related disorders.  Evodiamine isolated from Evodia rutaecarpa is cytotoxic to several cancer cell lines. Aims and Objectives  To isolate quinoline alkaloids from the Evodia and structure elucidation  To study their potential cytotoxicity, inhibitory activity of topoisomerase I and II, and catalytic mechanisms. Methods  Alkaloids were isolated using reverse-phase HPLC and the structures were elucidated by 1-D, 2-D NMR spectroscopy and mass spectrometry.  Their cytotoxicities were determined using MTT-based assay.  Topoisomerase (Topo) inhibitory activities were studied using Topo I relaxation and Topo II decatanation assay.  Comet assay and cell cycle distribution study were carried out by flow cytometry. Results and Discussion  Eight alkaloids were isolated from Evodia, of which evodiamine (Fig. 3) showed the most potent activity against human leukemia K562 and THP-1 cell lines (IC50 34.35 µM and 90.87 µM respectively.)  Evodiamine is an inhibitor for both human Topo I and Topo II (Fig. 4).  It did not induce DNA strand breaks at the cellular level (Fig. 5) and arrested cell growth at the G2/M phase in the cell cycle. N H N N O2 3 5 6 78 9 10 11 12 13 15 16 17 18 19 20 21 H H3C Conclusion Evodiamine was found to be a dual inhibitor for both topoisomerase I and II with potent cytotoxicity against human leukemia cell lines. Figure 1. Structure of topoisomerase Figure 2. Evodia rutaecarpa Figure 3. Structure of Evodiamine Figure 4. IC50 of evodiamine against human Topo I (A, C) using relaxation assay and Topo II (B, D) using decatanation assayFigure 5. The comet assay did not detect DNA strand breaks induced by evodiamine Acknowledgement X.Pan thanks the Department of Human Science at London Metropolitan University for a student bursary.