This document describes using the NUCAS (Nanjing University Carbon Assimilation System) adjoint-based data assimilation system to optimize ecosystem carbon and water fluxes by assimilating carbonyl sulfide (COS) and solar-induced fluorescence (SIF) observations. The NUCAS uses a two-leaf BEPS (Biosphere Energy-water-carbon with SIF) model and assimilates COS flux, SIF, and soil moisture observations to update model parameters. Preliminary twin experiments and single/multiple variable assimilation experiments at a Finnish forest site show NUCAS can improve simulations of COS fluxes, GPP, LH, SH by constraining plant carbon and soil hydraulic parameters. This represents the first attempt to