The document outlines the features and innovations of Applied Biosystems thermal cyclers, which have been serving the scientific community for over 30 years. It highlights capabilities such as high reaction yield, temperature accuracy, cloud-enabled access, and support services including repair and warranty options. Additionally, it emphasizes the instruments' reliability and their application in significant scientific discoveries, including advancements in molecular biology and autoimmune disease research.

1. The world leader in serving science
Applied Biosystems Thermal Cyclers

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Applied Biosystems™ Thermal Cyclers—Comprehensive Portfolio
* Also available as an FDA Class 1/CE-IVD labeled device.
ProFlex™ PCR
System
SimpliAmp™
Thermal Cycler
Veriti™ Thermal
Cycler*
MiniAmp™ Plus
Thermal Cycler
MiniAmp™
Thermal Cycler
Automated
Thermal Cycler
thermofisher.com/thermalcyclers
An instrument for every challenge, application, and budget
= cloud-enabled instrument

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Thermal cycler innovations
have enabled our customers
to make amazing discoveries
and advance science
Designing and manufacturing thermal cyclers since the first
commercial thermal cycler, the TC1 system, in 1987.
View complete timeline at
thermofisher.com/30yearsofinnovation
Applied Biosystems Thermal Cyclers—Innovating For Over 30 Years
Taq evolution
Use of a thermostable
DNA polymerase from
Thermus aquaticus (Taq) is
described using the TC1
Thermal Cycler, revolutionizing
molecular biology and the
analysis of DNA.
Turning point in the
fight against HIV
The chemokine receptor
CCR5 is identified as the
co-receptor for HIV infection
using the GeneAmp™ PCR
System 2400, leading to the
discovery of how HIV-infected
human cells function, and
why some individuals
are immune.
Understanding
autoimmune diseases
A common genetic variant that
confers susceptibility to
multiple autoimmune diseases
is identified using the
SimpliAmp Thermal Cycler,
implicating oxidative stress as
a potential driver of
autoimmune diseases.

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Learn more at
thermofisher.com/thermalcycleruniformity
Over the years, Applied Biosystems thermal cyclers have built a reputation for
temperature accuracy and uniformity.
For best results, we recommend using a thermal cycler with both high reaction yield and good well to-well uniformity (as indicated by low %CV).
Uniformity of PCR yield measured by %CV*
%CV
C1000™ T100™ Mastercycler™
Nexus Gradient
Mastercycler™
Pro S
SimpliAmpProFlex MiniAmp Plus MiniAmp
Bio-Rad Eppendorf Applied Biosystems / Thermo Fisher Scientific
10 µL plate 10.62 28.18 7.66 6.69 7.59 5.50 7.59 6.13
100 µL plate 7.54 9.87 7.16 14.74 7.30 5.93 7.30 6.79
Applied Biosystems Thermal Cyclers—Reputation for Performance
*PCR yields measured using Applied Biosystems™ Power SYBR™ Green PCR Master Mix (Cat. No. 4368577). %CV calculated as the average yield over three runs/standard deviation of the yield over three runs X100%.
0
5
10
15
20
25
30
Avg %CV 8.66

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Learn more at
thermofisher.com/thermalcyclerreliability
Test performed Test method Requirement Result
Component
reliability
Temperature cycles
1 cycle = 95°C (15 sec), 60°C
(60 sec)
>350,000 cycles Pass
Heated cover opening and
closing
Lid actuation robot:
1 cycle = close, open, close
>29,000 cycles Pass
Touchscreen touches
Touchscreen actuation robot:
1 cycle = touch, release
>2,900,000
touches
Pass
Docking mechanism
(ProFlex PCR System only)
Docking actuation robot:
1 cycle = dock, release, dock
>5,500 cycles Pass
Environmental
testing
Temperature
Chemistry performance in
environmental chamber
15–30°C Pass
Humidity
Chemistry performance in
environmental chamber
15–80% Pass
Elevation
Chemistry performance in
environmental chamber
6,000 ft (812 mbar) Pass
Shipping testing
ISTA-recommended shock and
vibration testing
Pass Pass
Durability and environmental testing results for Applied Biosystems thermal cyclers
Applied Biosystems Thermal Cyclers—Reputation for Reliability
Applied Biosystems
thermal cyclers adhere to
stringent reliability criteria

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Applied Biosystems Thermal Cyclers—Unique Features
Temperature zones
VeriFlex™ Block temperature control technology
More precise control over PCR optimization—each
block can be set with up to six specific temperatures
Precise incubation—use each temperature zone
of the VeriFlex Block for enzyme studies, restriction
digests, or any other process that requires precise
temperature control
Learn more about the technology at
thermofisher.com/veriflextechnology VeriFlex Blocks are constructed of
segmented metal blocks each with a
separate heating/cooling element

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Applied Biosystems Thermal Cyclers—Unique Features
Transition from other thermal cyclers quickly and
efficiently using ramp rate simulation modes
A library of modes that mimic the ramp rates of other
instruments is available on each Applied Biosystems
thermal cycler
Simulation mode

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Applied Biosystems Thermal Cyclers—Unique Features
Anywhere, anytime, with any mobile
device or desktop computer, you can:
Design and share protocols
Schedule an instrument*
Start or stop a run
Check run status*
Secure remote access with cloud-enabled instruments
* These features are available through the Instrument Connect app.

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Applied Biosystems Thermal Cyclers—Unique Features
thermofisher.com/fleetcontrol
Stay in control with Fleet Control Software
View and control thermal cyclers in your facility securely
behind your firewall with a central “server” computer
Create custom permissions and rules for
users, methods, and instruments
Quickly view your thermal cycler inventory and
availability right from the dashboard

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Applied Biosystems Thermal Cyclers—Which Instrument Is Right for You?
Our instruments enable precise, consistent results
for every challenge, application, and budget

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Compatible with Fleet Control Software
Applied Biosystems ProFlex PCR System
Ultimate flexibility and throughput
• Multi-user access—run three experiments at once with the
3 x 32 block option
• Flexible block configuration—accepts five different block formats for
optimization and throughput
• VeriFlex Blocks—six independent temperature zones for PCR optimization on
the 96-well block option
• Cloud-enabled—conveniently access your instrument anytime,
anywhere with Thermo Fisher Connect
3 x 32-well 96-well Dual 96-well Dual 384-well Dual flat
thermofisher.com/proflex
Block configuration options:

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Compatible with Fleet Control Software
Applied Biosystems SimpliAmp Thermal Cycler
Elegantly simple and precise
• Intuitive interface
Large 21 cm (8 in), easy-to-use color touchscreen for easy
programming and quick status checks
• VeriFlex Blocks
Three independent temperature zones for PCR optimization
• Cloud-enabled
Conveniently access your instrument anytime, anywhere
with Thermo Fisher Connect
thermofisher.com/simpliamp

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Tired of water baths?
Incubate samples at up to six different temperatures
simultaneously for enzyme activation studies, restriction digests,
or sequencing library preps with the Veriti Thermal Cycler.
Applied Biosystems Veriti Thermal Cycler
Proven reliability, precise PCR optimization
• VeriFlex Blocks
Six independent temperature zones for PCR optimization
• Easy-to-operate interface
Fast protocol setup and convenient protocol transfer with a
USB memory stick
thermofisher.com/veriti

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Applied Biosystems MiniAmp Plus and MiniAmp Thermal Cyclers
Routine PCR, elevated
thermofisher.com/miniamp
Compact design—fits in everyone’s lab space
at just 7.5 in. (19 cm) wide
Cloud-enabled—conveniently access your instrument
anytime, anywhere with Thermo Fisher Connect
MiniAmp
MiniAmp
Plus

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Compatible with Fleet Control Software
Applied Biosystems Automated Thermal Cycler
Designed for easy robotic integration
• Flexible modular design and small footprint
Helps save space on deck
• Automated lid
Easy, hands-free operation with a liquid handler or plate stacker
• Free desktop software
For PCR optimization prior to robotic integration
• Plug-and-play drivers and SiLA compatibility
For easy integration on your liquid handler of choice
thermofisher.com/atc

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Request a Thermal Cycler Demo Today
Request a demo today at
thermofisher.com/thermalcyclers
Download the comprehensive PCR and
Real-Time PCR Solutions brochure

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Applied Biosystems Thermal Cyclers—Service
Service Rapid Exchange
AB Repair
Center Support
Repair time
Replacement
shipped out in
1 business day
Your instrument
repaired and returned
in ~3 weeks
Off-site repair service
including shipping,
parts, and labor
● ●
Priority access to
technical support
● ●
Learn more at thermofisher.com/thermalcyclerservice
Flexible service plans provide the support
you need to keep your instrument running,
and may help you save up to 3x more.*
Most Applied Biosystems thermal cyclers
come with a two-year standard warranty.
Extended warranty packages are available,
as well as temperature verification and
qualification services.
* Savings realized with the Rapid Exchange plan on Veriti Thermal Cyclers, as compared to time and material (T&M) repairs. One-time T&M repair costs were calculated by parts and labor for major thermal cycler repair performed at global repair center. Cost
savings may vary by instrument and frequency of repair.

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Applied Biosystems Engineer-Approved PCR Plastics
Find the right plastic for your instrument at thermofisher.com/pcrplasticsselection
Applied Biosystems™ PCR plastics have been designed and validated
to work with Applied Biosystems thermal cyclers for more than 25 years.
Validated on Applied Biosystems thermal cyclers for optimal fit
and performance
Designed for optimal heat transfer with thin-walled
polypropylene wells
Designed to reduce cross-contamination with raised-well rims
for effective sealing

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Everything You Need For First-Class PCR Results
Reverse
transcription
PCR Nucleic acid
electrophoresis
Cloning Education
Learn more about the Applied Biosystems™ and Invitrogen™ collection of high-quality
instruments and reagents for the PCR workflow at thermofisher.com/amplifly

20. The world leader in serving science
Thank you
For Research Use Only. Not for use in diagnostic procedures.© 2018 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific
and its subsidiaries unless otherwise specified. C1000 and T100 are trademarks of Bio-Rad Laboratories. Mastercycler is a trademark of Eppendorf. COL16997 0818