Discover the innovations and more that led to amazing discoveries through the use of thermal cyclers. What were scientists able to accomplish? What things are important to them when selecting a thermal cycler? What do you need to advance your science?
Learn more about thermal cyclers: http://bit.ly/2Q2oPhF
See all thermal cycler offerings: http://bit.ly/2Paf1wH
This ppt is about biosensors. A brief Introduction to biosensors, history of Biosensors, working of biosensors, usage of biosensors application of biosensors in medical and other fields
Fabrication methods - Nanoscience and nanotechnologiesNANOYOU
An introduction to fabrication methods.
This chapter is part of the NANOYOU training kit for teachers.
For more resources on nanotechnologies visit: www.nanoyou.eu
Scale-up and scale-down of chemical processesSeppo Karrila
Explains the path from for example synthesizing a useful appearing material in the lab to actual production of the same. Also explains what pilot machines are, how they are used, and why sometimes down-scaling of a unit operation is done to experiment in bench-scale.
Protein based nanostructures for biomedical applications karoline Enoch
Proteins are kind of natural molecules that show unique
functionalities and properties in biological materials and
manufacturing feld. Tere are numerous nanomaterials
which are derived from protein, albumin, and gelatin. Tese
nanoparticles have promising properties like biodegradability, nonantigenicity, metabolizable, surface modifer, greater
stability during in vivo during storage, and being relatively
easy to prepare and monitor the size of the particles.
These particles have the ability to attach covalently with
drug and ligand
Biosensors in food industry’ presentation by Sonika Singh, NIFTEM, M.tech Fi...Sonika Singh
A presentation on 'Biosensors in Food Industry'. This presentation is the my work of status paper report on the same topic. Bio sensors usage in food industry and its prospects. The presentation is mostly pictorial but give a good idea about present scenario of usage of bio sensors in food industry in India with special focus on dairy and agriculture.
Mutagenesis; A conventional tool for strain improvement in industry Zohaib HUSSAIN
The strain improvement is the process of improvement and manipulation of microbial strains for the icreasment of metabolic level for industrial applications. The yield of microbial enzymes can be increased by using microbe specific medium for fermentation, improving the fermentation process and strain improvement for higher yield of product.
All these things lead to decrease in cost production. Microbe produce product according to its need therefore there is great need for overproduction. There is tremendous contribution of conventional Mutagenesis for strain improvement. Mutagenesis is important tool for the production of mutants which are capable to produce large product i.e. hyperactive.
Esco offers a choice of Conventional Thermal Cycler and Real Time Thermal Cycler models designed to meet critical requirements for all kinds of PCR processes, such as, Gradient PCR, Touch down PCR, High throughput PCR, in situ PCR and so on, using a variety of PCR tubes, strips, plates and slides. The Swift thermal cyclers use state of the art Peltier temperature control technology to achieve and maintain precise control and fast ramp rates with minimal over or under-shoot for process speed and accuracy.
This ppt is about biosensors. A brief Introduction to biosensors, history of Biosensors, working of biosensors, usage of biosensors application of biosensors in medical and other fields
Fabrication methods - Nanoscience and nanotechnologiesNANOYOU
An introduction to fabrication methods.
This chapter is part of the NANOYOU training kit for teachers.
For more resources on nanotechnologies visit: www.nanoyou.eu
Scale-up and scale-down of chemical processesSeppo Karrila
Explains the path from for example synthesizing a useful appearing material in the lab to actual production of the same. Also explains what pilot machines are, how they are used, and why sometimes down-scaling of a unit operation is done to experiment in bench-scale.
Protein based nanostructures for biomedical applications karoline Enoch
Proteins are kind of natural molecules that show unique
functionalities and properties in biological materials and
manufacturing feld. Tere are numerous nanomaterials
which are derived from protein, albumin, and gelatin. Tese
nanoparticles have promising properties like biodegradability, nonantigenicity, metabolizable, surface modifer, greater
stability during in vivo during storage, and being relatively
easy to prepare and monitor the size of the particles.
These particles have the ability to attach covalently with
drug and ligand
Biosensors in food industry’ presentation by Sonika Singh, NIFTEM, M.tech Fi...Sonika Singh
A presentation on 'Biosensors in Food Industry'. This presentation is the my work of status paper report on the same topic. Bio sensors usage in food industry and its prospects. The presentation is mostly pictorial but give a good idea about present scenario of usage of bio sensors in food industry in India with special focus on dairy and agriculture.
Mutagenesis; A conventional tool for strain improvement in industry Zohaib HUSSAIN
The strain improvement is the process of improvement and manipulation of microbial strains for the icreasment of metabolic level for industrial applications. The yield of microbial enzymes can be increased by using microbe specific medium for fermentation, improving the fermentation process and strain improvement for higher yield of product.
All these things lead to decrease in cost production. Microbe produce product according to its need therefore there is great need for overproduction. There is tremendous contribution of conventional Mutagenesis for strain improvement. Mutagenesis is important tool for the production of mutants which are capable to produce large product i.e. hyperactive.
Esco offers a choice of Conventional Thermal Cycler and Real Time Thermal Cycler models designed to meet critical requirements for all kinds of PCR processes, such as, Gradient PCR, Touch down PCR, High throughput PCR, in situ PCR and so on, using a variety of PCR tubes, strips, plates and slides. The Swift thermal cyclers use state of the art Peltier temperature control technology to achieve and maintain precise control and fast ramp rates with minimal over or under-shoot for process speed and accuracy.
We are manufacturers of environmental chambers in India
since last two decades. Our environmental test
chambers/environmental control chambers are
conceptualized and designed to create test chambers to
study and contemplate the effects of different environmental
parameters such as humidity, temperature and light in
various applications tests involving micro-organisms,
plants, tissues, electronic components etc. apart from
various customized industrial and research applications.
Our environmental chambers have a variety of usage in R &
D laboratories, research studies and product testing
facilities across the globe.
We are manufacturers of Hot air oven or laboratory oven in India since 1990. Our laboratory oven are widely used
for day to day heating and drying applications performed in various laboratories.
The OrbiCult™ Shaker have cutting edge technologies and robust mechanism suited for a wide range of options to meet an accurate and precise output. Provided with flexible accessories, intuitive interface and environmental friendly design - making sure that Esco laboratory shakers deliver outstanding feature and excellent performance.
CATEX™ 3 gas detection device is GAZOMAT™'s latest generation explosimeter-catharometer. This high precision detection technology ensures optimal safety when operating in hazardous atmospheres.
We are manufacturers of Incubator shaker or orbital shakers in India since 1990. Our incubator shakers are widely
used for day to day bacteriological and varied laboratory applications requiring incubation as well as shaking tests
and procedures in variety of research and testing laboratories.
PCR
PCR is a method widely used in Molecular biology to make many copies of a specific DNA segment.
Using PCR it is possible to generate thousand millions of copies of a particular section of DNA from very small amount of DNA.
PCR was originally developed in 1983 by the American Biochemists Kary Mullis. He was awarded the Nobel Prize in Chemistry in 1993 for his pioneering work.
CONSTANT TEMPERATURE HUMIDITY TEST CHAMBERacmasindia
WEIBER constant temperature humidity test creates
physiologically ideal surroundings for the perfect
environment stimulation in building physics and has wide
range of applications in material research, electronics,
physics, zoology and botany.
Positioned as micro centrifuge, MCV and refrigerated MCR stand out with excellent feature and versatility. MCV and MCR have a capacity of up to 44 x 1.5/2.0ml and can accommodate 12 x 5ml reaction tube or 4 x 8-pcr strips per run. With hematocrit rotor, it can accommodate 24 x capillaries per run for the determination of the hematocrit value.
It is designed with compact size to save space in the lab and with low noise performance that is preferred in any working area. The powerful refrigeration system of MCR keeps samples at temperature for as low as 4OC during a continuous run at 21380 x g.
Hot-start DNA polymerases are commonly used in PCR for genotyping, sequencing, molecular diagnostics, and high-throughput applications. In this presentation, PCR performance of Invitrogen™ Platinum II Taq Hot-Start DNA Polymerase and Invitrogen™ AccuPrime Taq DNA Polymerase is compared in the following areas:
• PCR run time for targets of different lengths
• Amplification of AT-rich and GC-rich sequences
• Tolerance to PCR inhibitors
• Sensitivity in target detection
• Universal protocol for PCR targets of different lengths
• Multiplex PCR of 15 targets
• Product format for direct gel loading
Request a sample of Platinum II Taq enzyme at http://bit.ly/2M4U9cw
Find other PCR enzymes at http://bit.ly/2JIPrzj
Learn more about PCR at http://bit.ly/2y2aSVo
#PCR #PCREducation #Invitrogen #InvitrogenSchoolofMolBio
Human cytomegalovirus (CMV) is a common immune-evasive herpes family virus leading to lifelong asymptomatic infection in 50 to 80% of humans. Current research evaluating the use of
TCR sequencing to predict response to immunotherapy has focused on measurements of T cell clonal expansion and TCR convergence (2,3,4) as potential predictive biomarkers for
response. Given that CMV infection has been reported to elicit large clonal proliferations of CMV reactive T cells (1), and is a source of chronic antigen stimulation, we hypothesized that CMV
infection might alter T cell repertoire features in a manner relevant to the potential biomarker use of TCR sequencing. Here we sought to identify features of CMV infection using TCRB profiling of
peripheral blood (PBL) total RNA. We identify reduced T cell evenness and elevated TCR convergence as features of chronic CMV infection.
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNAThermo Fisher Scientific
Tumor mutational burden (TMB) is a positive predictive factor for response to immune-checkpoint inhibitors in certain types of cancer. The Oncomine™ Tumor Mutation Load Assay, a targeted next generation sequencing (NGS) assay, measures TMB (from 1.2Mb of coding region) and detects mutations in 409 cancer genes. The TMB values obtained using targeted sequencing are highly correlated with TMB measured by whole exome sequencing. FFPE preservation methods can lead to significant cytosine deamination of the isolated DNA, resulting in decreased sequencing quality. In these samples, uracils are propagated as thymines and result in false C>T substitutions. Analysis of the Oncomine™ TML Assay using Torrent Suite and Ion Reporter ™ software uniquely estimates the degree of deamination in fixed tissues by measuring C:G>T:A variants. This deamination score is used to assess quality of DNA extracted from FFPE tumor tissue. To minimize the influence
that excess deamination has on TMB results, we have incorporated a repair treatment to eliminate damaged targets and improve usable TMB values of DNA from damaged FFPE tumor tissue using Uracil-DNA glycosylase (UDG). The
Oncomine™ TML Assay for TMB on the Ion Gene Studio™ S5 systems in conjunction with a deamination score is informative and potentially predictive for the use of checkpoint inhibitors in multiple cancer types.
What can we learn from oncologists? A survey of molecular testing patternsThermo Fisher Scientific
Oncologists are increasingly incorporating NGS testing to guide targeted and immuno-oncology therapies1. Most clinical NGS testing is confined to large academic institutions and reference labs, despite the fact that most cancer patients are treated in the community settings. We therefore sought to examine molecular testing selection patterns directly from oncologists in order to better identify perceived gaps in testing and treatment paradigms
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...Thermo Fisher Scientific
The use of cell-free circulating tumor DNA (ctDNA) for non-invasive cancer testing has the potential to revolutionize the field. However, emergence of an increasing number of extraction methods and detection assays is rendering laboratory workflow development much more complex and cumbersome. The use of standardized, well characterized ctDNA control materials in human plasma could facilitate the evaluation of extraction efficiency and assay performance across platforms. In this study, we use a full process ctDNA quality control material in true human plasma to demonstrate the variability of extraction yield between different ctDNA extraction kits. We also examine the correlation between the amplifiable
copy number and DNA concentration post-extraction.
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...Thermo Fisher Scientific
Presented here is an analytical validation of OCAv3 at the Life Technologies Clinical Services Laboratory (LTCSL), a CAP-accredited and CLIA-certified clinical laboratory. Analytical validations provide evidence of consistently accurate and relevant sequencing results.
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...Thermo Fisher Scientific
Accurate identification of uropathogens in a timely manner is important to correctly understand urinary tract infections(UTI’s), which affects nearly 150 million people each year. The
current standard approach for detecting the UTI pathogens is culture based. This method is time consuming, has low throughput, and can lack sensitivity and/or specificity. In addition, not all uropathogens grow equally well under standard culture conditions which can result in a failure to detect the species. To address these gaps, we have developed a unique workflow from sample preparation to target identification using the nanofluidic OpenArray™ platform for spatial multiplexing of target specific assays. In this study, we tested pre-determined blinded research samples and confirmed the subset of results with orthogonal Sanger sequences.
Liquid biopsy quality control – the importance of plasma quality, sample prep...Thermo Fisher Scientific
Liquid biopsy is emerging as a non-invasive companion to traditional solid tumor biopsies. As next generation sequencing (NGS) of circulating cell-free nucleic acids (cfNA = cfDNA and cfRNA) becomes common, it’s important to understand the impact of sample preparation on quality, specificity, and sensitivity of liquid biopsy tests. Plasma samples are often limited, and may have undesirable characteristics such as lipemia or hemolysis that contribute unwanted genomic DNA (gDNA) to the sample. Low cfDNA concentration can also limit the amount available for NGS library prep. In this study, we explore the effects of suboptimal plasma and low library input on liquid biopsy NGS, and discuss various techniques for in-process quality control of cfNA samples isolated from plasma
Streamlined next generation sequencing assay development using a highly multi...Thermo Fisher Scientific
Next generation sequencing (NGS) assay development for solid tumor sequencing requires characterization of variant calling directly from formalin-fixed paraffin embedded (FFPE) tissue samples. However, cell line based FFPE and human FFPE samples only contain 2 to 20 variants, which require laboratories to invest significant resources in sample sourcing and preparation when developing assays to detect 100+ variants
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...Thermo Fisher Scientific
T-cell receptor beta (TCRβ) immune repertoire analysis by next-generation sequencing is a valuable tool for studies of the tumor microenvironment and potential immune responses to cancer immunotherapy. Here we describe a TCRβ sequencing assay that leverages the low sample input requirements of AmpliSeq library preparation technology to extend the capability of targeted immune repertoire sequencing to include FFPE samples which can often be degraded and in short supply
Development of Quality Control Materials for Characterization of Comprehensiv...Thermo Fisher Scientific
Targeted next-generation sequencing (NGS) panels can detect hundreds of mutations in key genes using amplification based and hybrid-capture based NGS technologies. Although NGS technology is a powerful tool, optimizing and characterizing test performance on hundreds of variants is extremely challenging, time consuming, and expensive. Samples must be sourced, variants identified and orthogonally confirmed, then quantified and diluted. This effort is then multiplied across dozens of samples, and then samples must be run over many runs and days to assess assay reproducibility, precision, sensitivity, etc. In this study, we developed a novel reference material, experimental design, and analysis pipeline that allows for highly streamlined NGS assay characterization, enabling thorough test characterization across 500+ variants within only 6 runs.
As one of the leading causes of death globally, respiratory
infections could be caused by single or multiple types of viral,
bacterial or fungal pathogens that present in the upper and
lower respiratory tract. Panel-based testing using molecular
methods to identify multiple pathogens simultaneously can
contribute to better understanding of respiratory infections.
A high-throughput approach for multi-omic testing for prostate cancer researchThermo Fisher Scientific
The proliferation of genetic testing technologies and genome-scale studies has increased our understanding of the genetic basis of complex diseases. However, this information alone tells an incomplete story of the underlying biology. Integrative approaches that combine data from multiple sources, such as the genome, transcriptome and/or proteome, can provide a more comprehensive and multi-dimensional model of complex diseases. Similarly, the integration of multiple data types in disease screening can improve our understanding of disease in populations. In a series of groundbreaking multi-omic, population-based studies of prostate cancer, researchers at the Karolinska Institutet in Stockholm, Sweden identified sets of genetic and protein biomarkers that when evaluated together with other clinical research data performed significantly better in predicting cancer risk (1,2) than the most-widely used single protein biomarker, the prostate-specific antigen (PSA).
A rapid library preparation method with custom assay designs for detection of...Thermo Fisher Scientific
Herein, we describe a new research method for library
preparation using the Ion AmpliSeq™ HD Library Kit with
custom assay designs from Ion AmpliSeq HD Panels for
detection of low level variants from liquid biopsy samples. This
method includes incorporation of molecular tags that enable
0.1% Limit of Detection (LOD) in cell free DNA (cfDNA) and
dual barcodes for sample identification. This method is also
applicable to formalin-fixed paraffin embedded (FFPE)
samples. The libraries can be prepared in as little as 3 hours
and are compatible for analysis with the Ion GeneStudio™ S5
system
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...Thermo Fisher Scientific
Reprogramming permits the derivation of hiPSCs from diseased patients, and allows us to model diseases in vitro. Furthermore, with the advent of CRISPR mediated genome editing, we can now mimic disease mutations in control hiPSC lines to study the biological effect of just those mutations. hiPSCs can then be differentiated into specified cell types such as neurons which can be used to develop assays for drug safety screening or can be used to model disease phenotypes in a dish to discover new drugs.
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...Thermo Fisher Scientific
MicroRNAs (miRNA) are a class of small non-coding RNAs (approximately 21 nt long) that bind complementary sequences in target mRNAs to specifically regulate gene expression. Aberrant regulation of miRNAs and their targets has been associated with several diseases including cancer. The relationship between miRNA and mRNA has been found to be important in cancer development and progression. Simultaneous expression studies of miRNA and mRNA and detection of mutations in mRNA transcripts can be valuable in understanding molecular mechanisms that
have an underlying role in various diseases. We demonstrate the technical verification of a novel method to reverse-transcribe and pre-amplify miRNA and mRNA from sample-limiting serum research samples using the TaqMan® Advanced miRNA cDNA Synthesis Kit. Based on results from previous studies, a signature of 49 mRNA and 37 miRNA targets has been identified that may help distinguish between benign and malignant pancreatic tissues. In this study, these targets and an additional set of transcript mutations were analyzed in serum from normal and test samples. TaqMan assays for miRNA and mRNA targets and custom TaqMan Mutation Detection Assays (TMDAs) were placed on TaqMan Array Cards to facilitate investigation of several samples in a single experiment. Results demonstrate that transcript mutations can be detected and miRNA and mRNA targets can be reliably quantified from a single reverse transcription reaction. For research use only. Not for use in diagnostic purposes.
Identifying novel and druggable targets in a triple negative breast cancer ce...Thermo Fisher Scientific
In this study, we developed a CRISPR/Cas9-based high throughput loss-of-function screen for identifying target genes responsible for the tumor proliferation and growth in TNBC. Our initial focus was to identify essential kinases in MDA-MB-231 cell line using the Invitrogen™ LentiArray™ Human Kinase CRISPR Library, which targets 840 kinases with up to 4 different gRNAs per protein kinase for complete gene knockout. This functional screen identified over 90 protein kinases that are essential for cell viability and cell proliferation. Ten of these hits (CDK1, CDK2, CDK8, CDK10, CDK11A, CDK19, CDK19, CDC7, EPHA2 and WEE1) are well-known targets validated in the literature. Currently, we are in the process validating the novel hits through target gene sequencing, western blotting and target specific small molecule kinase inhibitors.
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...Thermo Fisher Scientific
T cell convergence refers to the phenomenon whereby antigen-driven selection enriches for T cell receptors (TCRs) having a shared antigen specificity but different amino acid or
nucleotide sequence. T cell recruitment and expansion within the tumor microenvironment (TME) may be directed by responses to tumor neoantigen, suggesting that elevated T
cell convergence could be a general feature of the tumor infiltrating T cell repertoire. Here we use the Ion AmpliSeq™ Immune Repertoire Assay Plus – TCRβ to evaluate evidence
for T cell convergence within melanoma tumor biopsy research samples from a set of 63 subjects plus peripheral blood leukocytes (PBL) from four healthy subjects. We find that the melanoma TME is highly enriched for convergent TCRs compared to healthy donor peripheral blood. We discuss the potential use of TCR convergence as a liquid biopsy compatible predictive biomarker for immunotherapy response.
Analytical performance of a novel next generation sequencing assay for Myeloi...Thermo Fisher Scientific
To support clinical and translational research into precision oncology strategies for myeloid cancers, a next-generation sequencing (NGS) assay was developed to detect common and relevant somatic alterations. To define gene targets that were recurrently altered in myeloid cancers and relevant for clinical and translational research, an extensive survey of investigators at hematology oncology research labs was performed.
Estimating Mutation Load from Tumor Research Samples using a Targeted Next-Ge...Thermo Fisher Scientific
Tumor mutation load predicts durable benefit from immune checkpoint inhibitors in several cancer types. Existing methods to estimate tumor mutation load have large input DNA and extensive infrastructure requirements and are associated with delays due to shipping biopsy samples to central laboratories. We demonstrate the ability of a targeted panel with fast
turn-around time and low input requirement for estimating mutation load from tumor samples to advance research in immuno-oncology.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Why is selecting the right thermal cycler important?
1. The world leader in serving science
Applied Biosystems Thermal Cyclers
2. 2
Applied Biosystems™ Thermal Cyclers—Comprehensive Portfolio
* Also available as an FDA Class 1/CE-IVD labeled device.
ProFlex™ PCR
System
SimpliAmp™
Thermal Cycler
Veriti™ Thermal
Cycler*
MiniAmp™ Plus
Thermal Cycler
MiniAmp™
Thermal Cycler
Automated
Thermal Cycler
thermofisher.com/thermalcyclers
An instrument for every challenge, application, and budget
= cloud-enabled instrument
3. 3
Thermal cycler innovations
have enabled our customers
to make amazing discoveries
and advance science
Designing and manufacturing thermal cyclers since the first
commercial thermal cycler, the TC1 system, in 1987.
View complete timeline at
thermofisher.com/30yearsofinnovation
Applied Biosystems Thermal Cyclers—Innovating For Over 30 Years
Taq evolution
Use of a thermostable
DNA polymerase from
Thermus aquaticus (Taq) is
described using the TC1
Thermal Cycler, revolutionizing
molecular biology and the
analysis of DNA.
Turning point in the
fight against HIV
The chemokine receptor
CCR5 is identified as the
co-receptor for HIV infection
using the GeneAmp™ PCR
System 2400, leading to the
discovery of how HIV-infected
human cells function, and
why some individuals
are immune.
Understanding
autoimmune diseases
A common genetic variant that
confers susceptibility to
multiple autoimmune diseases
is identified using the
SimpliAmp Thermal Cycler,
implicating oxidative stress as
a potential driver of
autoimmune diseases.
4. 4
Learn more at
thermofisher.com/thermalcycleruniformity
Over the years, Applied Biosystems thermal cyclers have built a reputation for
temperature accuracy and uniformity.
For best results, we recommend using a thermal cycler with both high reaction yield and good well to-well uniformity (as indicated by low %CV).
Uniformity of PCR yield measured by %CV*
%CV
C1000™ T100™ Mastercycler™
Nexus Gradient
Mastercycler™
Pro S
SimpliAmpProFlex MiniAmp Plus MiniAmp
Bio-Rad Eppendorf Applied Biosystems / Thermo Fisher Scientific
10 µL plate 10.62 28.18 7.66 6.69 7.59 5.50 7.59 6.13
100 µL plate 7.54 9.87 7.16 14.74 7.30 5.93 7.30 6.79
Applied Biosystems Thermal Cyclers—Reputation for Performance
*PCR yields measured using Applied Biosystems™ Power SYBR™ Green PCR Master Mix (Cat. No. 4368577). %CV calculated as the average yield over three runs/standard deviation of the yield over three runs X100%.
0
5
10
15
20
25
30
Avg %CV 8.66
5. 5
Learn more at
thermofisher.com/thermalcyclerreliability
Test performed Test method Requirement Result
Component
reliability
Temperature cycles
1 cycle = 95°C (15 sec), 60°C
(60 sec)
>350,000 cycles Pass
Heated cover opening and
closing
Lid actuation robot:
1 cycle = close, open, close
>29,000 cycles Pass
Touchscreen touches
Touchscreen actuation robot:
1 cycle = touch, release
>2,900,000
touches
Pass
Docking mechanism
(ProFlex PCR System only)
Docking actuation robot:
1 cycle = dock, release, dock
>5,500 cycles Pass
Environmental
testing
Temperature
Chemistry performance in
environmental chamber
15–30°C Pass
Humidity
Chemistry performance in
environmental chamber
15–80% Pass
Elevation
Chemistry performance in
environmental chamber
6,000 ft (812 mbar) Pass
Shipping testing
ISTA-recommended shock and
vibration testing
Pass Pass
Durability and environmental testing results for Applied Biosystems thermal cyclers
Applied Biosystems Thermal Cyclers—Reputation for Reliability
Applied Biosystems
thermal cyclers adhere to
stringent reliability criteria
6. 6
Applied Biosystems Thermal Cyclers—Unique Features
Temperature zones
VeriFlex™ Block temperature control technology
More precise control over PCR optimization—each
block can be set with up to six specific temperatures
Precise incubation—use each temperature zone
of the VeriFlex Block for enzyme studies, restriction
digests, or any other process that requires precise
temperature control
Learn more about the technology at
thermofisher.com/veriflextechnology VeriFlex Blocks are constructed of
segmented metal blocks each with a
separate heating/cooling element
7. 7
Applied Biosystems Thermal Cyclers—Unique Features
Transition from other thermal cyclers quickly and
efficiently using ramp rate simulation modes
A library of modes that mimic the ramp rates of other
instruments is available on each Applied Biosystems
thermal cycler
Simulation mode
8. 8
Applied Biosystems Thermal Cyclers—Unique Features
Anywhere, anytime, with any mobile
device or desktop computer, you can:
Design and share protocols
Schedule an instrument*
Start or stop a run
Check run status*
Secure remote access with cloud-enabled instruments
* These features are available through the Instrument Connect app.
9. 9
Applied Biosystems Thermal Cyclers—Unique Features
thermofisher.com/fleetcontrol
Stay in control with Fleet Control Software
View and control thermal cyclers in your facility securely
behind your firewall with a central “server” computer
Create custom permissions and rules for
users, methods, and instruments
Quickly view your thermal cycler inventory and
availability right from the dashboard
10. 10
Applied Biosystems Thermal Cyclers—Which Instrument Is Right for You?
Our instruments enable precise, consistent results
for every challenge, application, and budget
11. 11
Compatible with Fleet Control Software
Applied Biosystems ProFlex PCR System
Ultimate flexibility and throughput
• Multi-user access—run three experiments at once with the
3 x 32 block option
• Flexible block configuration—accepts five different block formats for
optimization and throughput
• VeriFlex Blocks—six independent temperature zones for PCR optimization on
the 96-well block option
• Cloud-enabled—conveniently access your instrument anytime,
anywhere with Thermo Fisher Connect
3 x 32-well 96-well Dual 96-well Dual 384-well Dual flat
thermofisher.com/proflex
Block configuration options:
12. 12
Compatible with Fleet Control Software
Applied Biosystems SimpliAmp Thermal Cycler
Elegantly simple and precise
• Intuitive interface
Large 21 cm (8 in), easy-to-use color touchscreen for easy
programming and quick status checks
• VeriFlex Blocks
Three independent temperature zones for PCR optimization
• Cloud-enabled
Conveniently access your instrument anytime, anywhere
with Thermo Fisher Connect
thermofisher.com/simpliamp
13. 13
Tired of water baths?
Incubate samples at up to six different temperatures
simultaneously for enzyme activation studies, restriction digests,
or sequencing library preps with the Veriti Thermal Cycler.
Applied Biosystems Veriti Thermal Cycler
Proven reliability, precise PCR optimization
• VeriFlex Blocks
Six independent temperature zones for PCR optimization
• Easy-to-operate interface
Fast protocol setup and convenient protocol transfer with a
USB memory stick
thermofisher.com/veriti
14. 14
Applied Biosystems MiniAmp Plus and MiniAmp Thermal Cyclers
Routine PCR, elevated
thermofisher.com/miniamp
Compact design—fits in everyone’s lab space
at just 7.5 in. (19 cm) wide
Cloud-enabled—conveniently access your instrument
anytime, anywhere with Thermo Fisher Connect
MiniAmp
MiniAmp
Plus
15. 15
Compatible with Fleet Control Software
Applied Biosystems Automated Thermal Cycler
Designed for easy robotic integration
• Flexible modular design and small footprint
Helps save space on deck
• Automated lid
Easy, hands-free operation with a liquid handler or plate stacker
• Free desktop software
For PCR optimization prior to robotic integration
• Plug-and-play drivers and SiLA compatibility
For easy integration on your liquid handler of choice
thermofisher.com/atc
16. 16
Request a Thermal Cycler Demo Today
Request a demo today at
thermofisher.com/thermalcyclers
Download the comprehensive PCR and
Real-Time PCR Solutions brochure
17. 17
Applied Biosystems Thermal Cyclers—Service
Service Rapid Exchange
AB Repair
Center Support
Repair time
Replacement
shipped out in
1 business day
Your instrument
repaired and returned
in ~3 weeks
Off-site repair service
including shipping,
parts, and labor
● ●
Priority access to
technical support
● ●
Learn more at thermofisher.com/thermalcyclerservice
Flexible service plans provide the support
you need to keep your instrument running,
and may help you save up to 3x more.*
Most Applied Biosystems thermal cyclers
come with a two-year standard warranty.
Extended warranty packages are available,
as well as temperature verification and
qualification services.
* Savings realized with the Rapid Exchange plan on Veriti Thermal Cyclers, as compared to time and material (T&M) repairs. One-time T&M repair costs were calculated by parts and labor for major thermal cycler repair performed at global repair center. Cost
savings may vary by instrument and frequency of repair.
18. 18
Applied Biosystems Engineer-Approved PCR Plastics
Find the right plastic for your instrument at thermofisher.com/pcrplasticsselection
Applied Biosystems™ PCR plastics have been designed and validated
to work with Applied Biosystems thermal cyclers for more than 25 years.
Validated on Applied Biosystems thermal cyclers for optimal fit
and performance
Designed for optimal heat transfer with thin-walled
polypropylene wells
Designed to reduce cross-contamination with raised-well rims
for effective sealing
19. 19
Everything You Need For First-Class PCR Results
Reverse
transcription
PCR Nucleic acid
electrophoresis
Cloning Education
Learn more about the Applied Biosystems™ and Invitrogen™ collection of high-quality
instruments and reagents for the PCR workflow at thermofisher.com/amplifly