Thermal cyclers have become much more affordable over time. The author's first lab paid over $10,000 for a basic thermal cycler, whereas now quality machines can be found for under $5,000. An interlaboratory study evaluated the performance of 33 thermal cyclers from different manufacturers in controlled PCR and RAPD analyses. It found that temperature-calibrated cyclers consistently produced more repeatable results than non-calibrated ones, irrespective of make and model. Proper temperature calibration and monitoring are important for thermal cycler performance. Extended warranty and service are also important factors to consider when selecting a reliable unit.