The laboratory aimed to separate proteins expressed in E. coli after transformation using SDS-PAGE. Samples from bacteria colonies containing the pGLO plasmid were prepared with and without heat treatment. The samples were run on a polyacrylamide gel, which was then divided and stained with Coomassie blue or left unstained. The unstained gel showed fluorescence at 37kD in lane 8, while lanes 9-10 showed complete protein denaturation. The stained gel demonstrated a variety of E. coli proteins from 10-160kD and showed GFP denaturation at 27kD and partial denaturation at 37kD in lanes 3 and 5.