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Micro-206 Mid Key (This is just a review by a student to memorize. You MUST have to consult notes)
Genera Species Characteristics Virulence Diseases Diag+Treatment
Staphylococcus Gm +ve, tetrads
S.aureus GoldYell,coag +ve
S.albus white
S.citrus lemon yell
Coa–ve S.epidermidis
S.saprophyticus
S.capitis,S.haemolytics
Micrococcus,Stomatococcus
Alloiococcus otitidis
0.5-1um
Non motile
Aerobic
/fucl anaerobe
Cata+ve
10% NaCl 18-40C
Skin&Mucous
membrane
1.Cell wall asso struc
Pept.gly,caps,pro-A,
Bound coagulase
2.E/c toxins
Haemolysin,leukocidin
Enterotoxin,TSST
Exfoliation Toxin
3.E/C enz,s.kinase
DNase,Phosphatase,lipase
Phospholipase,hyaluronidase
Serokinase,protease
Dog/Cat:Canine
pyoderma:S.interm,
Otitis externa:S.inte,
Mastitis:S.inter
Bovines:Mastitis:S.
aureus+ other spp
Pyemia f lambs:tick
Equines:Mastitis:aur
Pectoraelabcess:aure
Sper cord:S.aureus
Avian:Bumble foot
Diag:Aspirates of
unopened lesions
Milk,blood,urine
Gm stn > +Ve
Blood agar:Beta
Toxins,
Resis to:
penG,Streptomy,tetra so
fluoroquinolones
Cephalosporins used
Streptococcus Beta-haemeolytic:clear zone
S.pyogenes,S.equi,S.agalactiae
alpha-Haemolytic:green zone
S.pneumoniae,nonhaemolytic
Viridians streptococci
Y/Non-haemolytic:S.faecalis
mostly non pathogenic
fucl anaerobes,LF
cata-v,enrichment
medium:bld,serum
capsulated,N-acetyl
glucosamine,Nacetyl
muramic Acid,
6.5%NaCl,40% bile
Structural: M.Protein
Antibody pro to it:Normal E/C
products: Erythrogenic
toxins,streptolysin O&S,
Streptokinase(fibrinolysin)
DNase,Hyaluronidase
Dog/cat:S.canis 2ndry
pneumonias
Ruminants:Mastitis:
S.agal,S.dysgalactiae
Equines:Strangles:
S.equi,Swines:servical
lymphadenitis
CAMP(Christie,atkins
Munch-peterson) factor
test
Bacillus fucl an,15-40C,5%
CO2,Serum agar
HCO3, Gm+, non-
motile,rods with
squarends,spores,D-
Glutamylpolypeptide
Protein para-
crystalline (S-layer)
Capsule
Lethal toxins:pX01>
PA+LF,Edema toxin
pXO1>PA+EF
S-layer>non patho
B.cereus: Emetic,secretory
enterotoxin(HBL,NHE,T)
B.anthracis:ruminants
High fvr,agalactia
Abortion.horses:Horse
Colic & Diarrhea
Swine:lymphadenitis &
ulcerative lesion
B.cereus:bovine mastitis
Dia:blood,spleen
Resis to:Quaternary
ammonium disinfect
phenolic+alcoholic,
susceptible to:pen,
chloramphanicol,strp,
tetra
Listeria L.monocytogenes,L.ivanovii,
L.innocua,L.grayi,L.seeligeri,
L.welshimeri,
Fucl an,Gm+,
4-45C,30-37-best
Non Acid fast,Non-
spor,motile@22C,
poor at 37,O-Ag wall
Meso-
diaminopimelic
acid,tolerates pH
5.5-
9.6,10%NaCl,40%bil
e
Adhesin(ActA),internalins,
Haemolysin,listeriolysin O
(major),phospholipases C
Septicaemia,
Ruminanats:encepha-
litis,abortion,conjuncti-
vitis,CNS infection
through Oral,nasal/
ocular mucosal surfaces,
Specimens:Spinal
fluid,blood,brain
tissue,liver spleen,
Abomasal fluid
CAMP+ Crossed
streakedwithS.aureus
Antibiotics:Peni,
Ampi,erythro,tetra,
Mycobacterium M.Tb>TB man,
M.bovis,cattle
M.avium,
Rod
0.5um,Gm+,acid
Fast+,auramine-
rhodamine stained,
Resist discoloration
with 3% HCl,
Alkyl hydroperoxidase
reductase,dimycolyl
trehalose,iron acquisition
pro(mycobactins,exochelins)
Sulfolipids,PIM,surface
mycosides
Cattle:resp infection
Involving lymph nodes
& serous cavities,
Horses;rarely,gross tumor
like lesions,
Swine:alimentarycanal
Tracheobronchial &
Gastric lavages,
Lymph nodes,thoracic
abd respirates,urine
faeces,biopsy
Streptomycin,iso-
Niazid,ethambutol,
riphampin
Corynebacterium C.pseudotuberculosis
Grwth chrctr:fucl ana,blood
/serum best,48hrs 35-37ºC,off-
White,dull,faint hemolytic col,
1mm dia,hockey puck,cata+ve
Diphtheroid,0.5-0.3µ
Non-acid fast,no
cap,
Wall:gm+,DAP,arab
i-nogalactin,mycolic
acid,high lipids
Cell wall:PS+Lipids,
lipoteichoic acids,Pep-gly,
Exotoxins:PLD,serine
protease,
Iron acquisition:fagA-D
Eq:Pectoral abcesses:
pigeon fvr,brstbon fvr,
Sheep&Goat:lymphad-
enitis,onion ring,
cattle:skin+lym nod
Peni,eryth,chloram,
Resistant to
aminoglycosides,
Equin biotype:NO3+
Ovine biotype:NO3 -
C.renale Group C.renale,C.cystidis,C.pilosum
Grwth chrctr:fucl ana,non-
hemolytic,opaque,off-white col,
48hrs at 37ºC,glucose:slowly
Acidified,cata+,
Renalin: +CAMP ,
Other species:Actinobaculum&
C.auricanis(No Keypoints
mentioned here,
As Above Adhesins:fibrillar(pilli,fimbr)
C.Wall:As Above,
Urease:urea-NH3
NH3 initiates infl procs,
Urine pH=9,Cattle:
Bovine pyelonephritis
,thick bladder+uretral
walls,distended ureters
Small Rum:Pizzle rot
Necrotizinginflammaofpre
puce
&adjacent issues
Diag:RBCs+9pH of
urine,Christensens
Urea agar slant:an
Alkaline shift,
Early stage: penicillin
Ovine posthitis:
surgical care,
antiseptic,diet restrict,
testosterone adminstrd
Actinomycetes
25 spp cata-ve
(A.viscosus,A.caniscata+)Grwth
chrctr:capnoophilic+fucl ana,
Serum/blood,no on sabouraud
35-37C 48hrs,
Found on oral mucosa,tooth
surfac,urinognital tract mucosa
Gm+,diphtheroids/fila
0.5um,C.wall:surface
fibrils,adhesions,surfa
ce
Ags,
Evoke pyogranulomatous
reaction with unknown
mechanisms,mononuclear
infilterations+fibrosis
Sinus tracts,Sulfur Granules
Rum:lumpy jaw
(A.bovis,A.israelii)
Horses:Poll evil&
Fistulous withers,cervi-cal
lymphadenitis,
Dog&Cat:serositidis
Discospondylitis
Swines:Mastitis,
Pneumonia,abortion
Samples:aspirates from
unopened lesion
Sulfur Granules:yellow
Particles,rosettes
Treatment:peni+ami-
noglycosides=bovine,
Dog&cat:surgery
Nocardia 30 spp
N.asteroides,
N.abcessus,
N.farcinica,
N.nova,
N.transvalensis.
Gm+,DAP,mycolic
acid
Arabinogalatin,
Obligate
aer,sabouraud
10-50C,opaque
col,surface-waxy-
powdery-velvety,dia-
cm
NO Exotoxin but
Superoxide dismutase
sup-ox-dismutase+lysosomal
enz-Survive within phagocytic
vacuoles,lipids-initiating
granulomatous reac,
Suppurative process-
granulomatous features
Rum:Mastitis,Bovine
farcy,pneu,abor,ly.ad
Hor:nocardioform
placentitis,
Dog-cat:febrile illness,
Pleuritis,empyema,diss-
mination to
organs,Mycetoma,
Dia:Granules:sometime
Blood agar,37C
Dull opaque,waxy
velvety,hard to dislodge
Cata+,
trimethoprim-
sulfonamide therapy,
resistant to
fluoroquinolones,
Dermatophilus D.congolensis
Grwth chrctr:on blood&
infusion media,aer+capno,
Hemolytic:waxy,viscous,whitish
gray-yellow,smooth-wrinkled
Catalase,urease,protease:+
Motile coccoid
zoospore
2um dia,C.wall:DAP,
Lack glycin &
arabino-galactin
Patho:Exudativeepidermatitis,
spores germination-inflam,
scabs,
non-epidermal-
tongue,u.bladr,lymph
nod,subcutis
Equine rain scald,
Ovine strwbry foot rot,
Equine grease heel
Lumpy wool
Dia:branching
filamentsZoospore
resembling cocci,blood
agar 5-10%CO2
35-37C 48 hours-
peniG,tetra,chloram,
Clostridium C.perfringens(Type A to E)
General Growth characters of
all species:anaerobic envrmnt,
2-10%CO2,Complex media
blood/serum,1-2days incubation
Swarm across moist agar
without forming colonies,
Hemolysis shown
Gm+,spore
forming,non
Motile,encaps,anaero
be,
1.Adhesins:2fibronectin
binding pro+collagen bind,
2.Capsule:wounds(gangrene)
3.Toxins: Cpa/Plc,Cpb,Cpb2,
Etx,Itx,Col(kappa),Mu(Nag)
Theta,Nan(Sialidase),hemolys
in
Gas gangrene(Wound)
Enterotoxemias
Ent-A:gastritis,enteritis
Hemolytic yellow lamb
Disease,Ent-B:old wor-ld
dis,lamb dysentry
Passive & Active immu,
Dia:bloodagar=α-toxn
Hemolysis,clotting milk
Treat: bacterin+Toxoid
Metronidazole,
Macrolides,ampicillin
C.novyi(Type A,B,C) Non-encapsulated,Gm+,
Motile,anaerobic,rod,spore
Exotoxins
1.α-toxin,2.β-toxin,-toxin
TypeA:gas gangrene of
humans,wounds in
animals,big-head of rams,
TypeB:black disease-sheep
cattle horses
TypeC:Osteomyelitis of
water buffalo
Dia:Large Gm+-gram
variable rods,spores
Identified by
fluorescent
Nutritionally fastidious
Treat:Prophylactic
vaccination,eliminating
Flukes+other hepato-
pathogenic agents
C.haemolyticum Non-encap,motile,strict
anaerobe,spores,resembles
C.novyi typeB
Toxin: Phospholipase C(β-
toxin)-hemolytic crisis,
Liver infarcts,fever,pale
Icteric mucous
membranes,anorexia
agalactiahaemoglobin-,
uria(red water disease)
Dia:Liver lesions,Gm
stain,FAT,blood agar,
Treat:Broad spec
antibio,antitoxins,blood
Transfusion,
C.chauvoei Gm+,motile,obligate
anaerobic,rod,subterminal
or subcentral spores
Exotoxins:alpha,beta,gamma
Delta(chauveolysin),
Neuramninidase(sialidase)
α,,+Neuraminidase=
initial lesions,Black-leg
(edema,hemorrhage,
myofibrillar necrosis,
Dia:Gm+,FAT,media-
Cystein & water
soluble
Vitamins,Treat:peni I/V
Cattle:3-6 months vacc
C.difficile Gm+,motile,encapsulated,
Spore-forming anaerobic
rod,
Adhesins:pilli+fimbrae,cell
wall protein>Cwp66
Capsule:CHO Capsule
Toxins:A(enterotoxins),
B(Cytolysin),ADP-ribosyltra-
nsferase
Adhesion to mucous/epi
Cells of LI,death of
cells,prostaglandins,
Antibiotics & drugs
associated diarrhea,
Dia:PCR,serology,
Isolated CCFA medium
C.botulinum Gm+,spore-forming,
Anaerobic rod,at pH near
or above neutral>
subterminal oval spores
Toxin:Botulinum toxin(BoNT)
zinc endopeptidase,C2
Toxin,C3 exoenzyme,
BoNT absorbed-blood-
recptors-nerve cell-mucular
incoordination
-recumbancy+Tongue
extrusion+oral probs
Bird-Limberneck disease
Dia:plasma or tissue
toxin,iso frm intestine,
feedstuff,stomach
Treat:Antitoxin,toxoid
(ABCD,guanidine,
Aminopyridine->Ach
C.tetani
Swarming growth,CHO ferm,
Proteolysis,indole production
Gm+,spore-forming,
Anaerobic rod,spherical
spores(drumstick,racket)
1.Tetanustoxin(tetanosapsmin)
(TeNT)2.Tetanolysin
Mortality at least 50%
(Hor,rum,swine):descending
tetanus,bloat in rum,lock-
jaw,sawhorse,tetanic spasm
Carni:ascending
Dia:increased agar-
Inhibits swarming,37C
4days,PCR
Treat:Antitoxin,peni
Various Bacterial Genera of Veterinary Significance (1)

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  • 1. Micro-206 Mid Key (This is just a review by a student to memorize. You MUST have to consult notes) Genera Species Characteristics Virulence Diseases Diag+Treatment Staphylococcus Gm +ve, tetrads S.aureus GoldYell,coag +ve S.albus white S.citrus lemon yell Coa–ve S.epidermidis S.saprophyticus S.capitis,S.haemolytics Micrococcus,Stomatococcus Alloiococcus otitidis 0.5-1um Non motile Aerobic /fucl anaerobe Cata+ve 10% NaCl 18-40C Skin&Mucous membrane 1.Cell wall asso struc Pept.gly,caps,pro-A, Bound coagulase 2.E/c toxins Haemolysin,leukocidin Enterotoxin,TSST Exfoliation Toxin 3.E/C enz,s.kinase DNase,Phosphatase,lipase Phospholipase,hyaluronidase Serokinase,protease Dog/Cat:Canine pyoderma:S.interm, Otitis externa:S.inte, Mastitis:S.inter Bovines:Mastitis:S. aureus+ other spp Pyemia f lambs:tick Equines:Mastitis:aur Pectoraelabcess:aure Sper cord:S.aureus Avian:Bumble foot Diag:Aspirates of unopened lesions Milk,blood,urine Gm stn > +Ve Blood agar:Beta Toxins, Resis to: penG,Streptomy,tetra so fluoroquinolones Cephalosporins used Streptococcus Beta-haemeolytic:clear zone S.pyogenes,S.equi,S.agalactiae alpha-Haemolytic:green zone S.pneumoniae,nonhaemolytic Viridians streptococci Y/Non-haemolytic:S.faecalis mostly non pathogenic fucl anaerobes,LF cata-v,enrichment medium:bld,serum capsulated,N-acetyl glucosamine,Nacetyl muramic Acid, 6.5%NaCl,40% bile Structural: M.Protein Antibody pro to it:Normal E/C products: Erythrogenic toxins,streptolysin O&S, Streptokinase(fibrinolysin) DNase,Hyaluronidase Dog/cat:S.canis 2ndry pneumonias Ruminants:Mastitis: S.agal,S.dysgalactiae Equines:Strangles: S.equi,Swines:servical lymphadenitis CAMP(Christie,atkins Munch-peterson) factor test Bacillus fucl an,15-40C,5% CO2,Serum agar HCO3, Gm+, non- motile,rods with squarends,spores,D- Glutamylpolypeptide Protein para- crystalline (S-layer) Capsule Lethal toxins:pX01> PA+LF,Edema toxin pXO1>PA+EF S-layer>non patho B.cereus: Emetic,secretory enterotoxin(HBL,NHE,T) B.anthracis:ruminants High fvr,agalactia Abortion.horses:Horse Colic & Diarrhea Swine:lymphadenitis & ulcerative lesion B.cereus:bovine mastitis Dia:blood,spleen Resis to:Quaternary ammonium disinfect phenolic+alcoholic, susceptible to:pen, chloramphanicol,strp, tetra
  • 2. Listeria L.monocytogenes,L.ivanovii, L.innocua,L.grayi,L.seeligeri, L.welshimeri, Fucl an,Gm+, 4-45C,30-37-best Non Acid fast,Non- spor,motile@22C, poor at 37,O-Ag wall Meso- diaminopimelic acid,tolerates pH 5.5- 9.6,10%NaCl,40%bil e Adhesin(ActA),internalins, Haemolysin,listeriolysin O (major),phospholipases C Septicaemia, Ruminanats:encepha- litis,abortion,conjuncti- vitis,CNS infection through Oral,nasal/ ocular mucosal surfaces, Specimens:Spinal fluid,blood,brain tissue,liver spleen, Abomasal fluid CAMP+ Crossed streakedwithS.aureus Antibiotics:Peni, Ampi,erythro,tetra, Mycobacterium M.Tb>TB man, M.bovis,cattle M.avium, Rod 0.5um,Gm+,acid Fast+,auramine- rhodamine stained, Resist discoloration with 3% HCl, Alkyl hydroperoxidase reductase,dimycolyl trehalose,iron acquisition pro(mycobactins,exochelins) Sulfolipids,PIM,surface mycosides Cattle:resp infection Involving lymph nodes & serous cavities, Horses;rarely,gross tumor like lesions, Swine:alimentarycanal Tracheobronchial & Gastric lavages, Lymph nodes,thoracic abd respirates,urine faeces,biopsy Streptomycin,iso- Niazid,ethambutol, riphampin Corynebacterium C.pseudotuberculosis Grwth chrctr:fucl ana,blood /serum best,48hrs 35-37ºC,off- White,dull,faint hemolytic col, 1mm dia,hockey puck,cata+ve Diphtheroid,0.5-0.3µ Non-acid fast,no cap, Wall:gm+,DAP,arab i-nogalactin,mycolic acid,high lipids Cell wall:PS+Lipids, lipoteichoic acids,Pep-gly, Exotoxins:PLD,serine protease, Iron acquisition:fagA-D Eq:Pectoral abcesses: pigeon fvr,brstbon fvr, Sheep&Goat:lymphad- enitis,onion ring, cattle:skin+lym nod Peni,eryth,chloram, Resistant to aminoglycosides, Equin biotype:NO3+ Ovine biotype:NO3 - C.renale Group C.renale,C.cystidis,C.pilosum Grwth chrctr:fucl ana,non- hemolytic,opaque,off-white col, 48hrs at 37ºC,glucose:slowly Acidified,cata+, Renalin: +CAMP , Other species:Actinobaculum& C.auricanis(No Keypoints mentioned here, As Above Adhesins:fibrillar(pilli,fimbr) C.Wall:As Above, Urease:urea-NH3 NH3 initiates infl procs, Urine pH=9,Cattle: Bovine pyelonephritis ,thick bladder+uretral walls,distended ureters Small Rum:Pizzle rot Necrotizinginflammaofpre puce &adjacent issues Diag:RBCs+9pH of urine,Christensens Urea agar slant:an Alkaline shift, Early stage: penicillin Ovine posthitis: surgical care, antiseptic,diet restrict, testosterone adminstrd
  • 3. Actinomycetes 25 spp cata-ve (A.viscosus,A.caniscata+)Grwth chrctr:capnoophilic+fucl ana, Serum/blood,no on sabouraud 35-37C 48hrs, Found on oral mucosa,tooth surfac,urinognital tract mucosa Gm+,diphtheroids/fila 0.5um,C.wall:surface fibrils,adhesions,surfa ce Ags, Evoke pyogranulomatous reaction with unknown mechanisms,mononuclear infilterations+fibrosis Sinus tracts,Sulfur Granules Rum:lumpy jaw (A.bovis,A.israelii) Horses:Poll evil& Fistulous withers,cervi-cal lymphadenitis, Dog&Cat:serositidis Discospondylitis Swines:Mastitis, Pneumonia,abortion Samples:aspirates from unopened lesion Sulfur Granules:yellow Particles,rosettes Treatment:peni+ami- noglycosides=bovine, Dog&cat:surgery Nocardia 30 spp N.asteroides, N.abcessus, N.farcinica, N.nova, N.transvalensis. Gm+,DAP,mycolic acid Arabinogalatin, Obligate aer,sabouraud 10-50C,opaque col,surface-waxy- powdery-velvety,dia- cm NO Exotoxin but Superoxide dismutase sup-ox-dismutase+lysosomal enz-Survive within phagocytic vacuoles,lipids-initiating granulomatous reac, Suppurative process- granulomatous features Rum:Mastitis,Bovine farcy,pneu,abor,ly.ad Hor:nocardioform placentitis, Dog-cat:febrile illness, Pleuritis,empyema,diss- mination to organs,Mycetoma, Dia:Granules:sometime Blood agar,37C Dull opaque,waxy velvety,hard to dislodge Cata+, trimethoprim- sulfonamide therapy, resistant to fluoroquinolones, Dermatophilus D.congolensis Grwth chrctr:on blood& infusion media,aer+capno, Hemolytic:waxy,viscous,whitish gray-yellow,smooth-wrinkled Catalase,urease,protease:+ Motile coccoid zoospore 2um dia,C.wall:DAP, Lack glycin & arabino-galactin Patho:Exudativeepidermatitis, spores germination-inflam, scabs, non-epidermal- tongue,u.bladr,lymph nod,subcutis Equine rain scald, Ovine strwbry foot rot, Equine grease heel Lumpy wool Dia:branching filamentsZoospore resembling cocci,blood agar 5-10%CO2 35-37C 48 hours- peniG,tetra,chloram, Clostridium C.perfringens(Type A to E) General Growth characters of all species:anaerobic envrmnt, 2-10%CO2,Complex media blood/serum,1-2days incubation Swarm across moist agar without forming colonies, Hemolysis shown Gm+,spore forming,non Motile,encaps,anaero be, 1.Adhesins:2fibronectin binding pro+collagen bind, 2.Capsule:wounds(gangrene) 3.Toxins: Cpa/Plc,Cpb,Cpb2, Etx,Itx,Col(kappa),Mu(Nag) Theta,Nan(Sialidase),hemolys in Gas gangrene(Wound) Enterotoxemias Ent-A:gastritis,enteritis Hemolytic yellow lamb Disease,Ent-B:old wor-ld dis,lamb dysentry Passive & Active immu, Dia:bloodagar=α-toxn Hemolysis,clotting milk Treat: bacterin+Toxoid Metronidazole, Macrolides,ampicillin
  • 4. C.novyi(Type A,B,C) Non-encapsulated,Gm+, Motile,anaerobic,rod,spore Exotoxins 1.α-toxin,2.β-toxin,-toxin TypeA:gas gangrene of humans,wounds in animals,big-head of rams, TypeB:black disease-sheep cattle horses TypeC:Osteomyelitis of water buffalo Dia:Large Gm+-gram variable rods,spores Identified by fluorescent Nutritionally fastidious Treat:Prophylactic vaccination,eliminating Flukes+other hepato- pathogenic agents C.haemolyticum Non-encap,motile,strict anaerobe,spores,resembles C.novyi typeB Toxin: Phospholipase C(β- toxin)-hemolytic crisis, Liver infarcts,fever,pale Icteric mucous membranes,anorexia agalactiahaemoglobin-, uria(red water disease) Dia:Liver lesions,Gm stain,FAT,blood agar, Treat:Broad spec antibio,antitoxins,blood Transfusion, C.chauvoei Gm+,motile,obligate anaerobic,rod,subterminal or subcentral spores Exotoxins:alpha,beta,gamma Delta(chauveolysin), Neuramninidase(sialidase) α,,+Neuraminidase= initial lesions,Black-leg (edema,hemorrhage, myofibrillar necrosis, Dia:Gm+,FAT,media- Cystein & water soluble Vitamins,Treat:peni I/V Cattle:3-6 months vacc C.difficile Gm+,motile,encapsulated, Spore-forming anaerobic rod, Adhesins:pilli+fimbrae,cell wall protein>Cwp66 Capsule:CHO Capsule Toxins:A(enterotoxins), B(Cytolysin),ADP-ribosyltra- nsferase Adhesion to mucous/epi Cells of LI,death of cells,prostaglandins, Antibiotics & drugs associated diarrhea, Dia:PCR,serology, Isolated CCFA medium C.botulinum Gm+,spore-forming, Anaerobic rod,at pH near or above neutral> subterminal oval spores Toxin:Botulinum toxin(BoNT) zinc endopeptidase,C2 Toxin,C3 exoenzyme, BoNT absorbed-blood- recptors-nerve cell-mucular incoordination -recumbancy+Tongue extrusion+oral probs Bird-Limberneck disease Dia:plasma or tissue toxin,iso frm intestine, feedstuff,stomach Treat:Antitoxin,toxoid (ABCD,guanidine, Aminopyridine->Ach C.tetani Swarming growth,CHO ferm, Proteolysis,indole production Gm+,spore-forming, Anaerobic rod,spherical spores(drumstick,racket) 1.Tetanustoxin(tetanosapsmin) (TeNT)2.Tetanolysin Mortality at least 50% (Hor,rum,swine):descending tetanus,bloat in rum,lock- jaw,sawhorse,tetanic spasm Carni:ascending Dia:increased agar- Inhibits swarming,37C 4days,PCR Treat:Antitoxin,peni